The functional role of threonine-205 in the mechanism-based inactivation of P450 2B1 by two ethynyl substrates: The importance of the F helix in catalysis

Journal of Pharmacology and Experimental Therapeutics

Volumn 311, Issue 3, 2004, Pages 855-863

  Lin, Hsia Lien ^a   Kent, Ute M ^a   Zhang, Haoming ^a,b   Waskell, Lucy ^a,b   Hollenberg, Paul F ^a,c  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b VA ANN ARBOR HEALTHCARE SYSTEM   (United States)

^c 2301 MSRB III ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 ETHYNYLNAPHTHALENE; 2 NAPHTHYLACETIC ACID; 7 ETHOXY (TRIFLUOROMETHYL)COUMARIN; ALANINE; APOPROTEIN; COUMARIN DERIVATIVE; CYTOCHROME P450 2B1; ETHINYLESTRADIOL; FLUORINE DERIVATIVE; NAPHTHALENE DERIVATIVE; SERINE; TESTOSTERONE; THREONINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL REACTION; COMPARATIVE STUDY; COVALENT BOND; DEETHYLATION; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; F HELIX; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; ISOTOPE LABELING; METABOLISM; METABOLITE; MUTANT; MUTATION; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; APOPROTEINS; AUTORADIOGRAPHY; BINDING SITES; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP2B1; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ETHINYL ESTRADIOL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; KINETICS; MODELS, MOLECULAR; NAPHTHALENES; PROTEIN CONFORMATION; THREONINE; VALINE;

EID: 10044219534     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: 10.1124/jpet.104.071670     Document Type: Article

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