Prima Facie Evidence That a Phytocystatin for Transgenic Plant Resistance to Nematodes Is Not a Toxic Risk in the Human Diet

Journal of Nutrition

Volumn 134, Issue 2, 2004, Pages 431-434

  Atkinson, Howard J ^a   Johnston, Katherine A ^a   Robbins, Mark ^b  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b BIBRA INTERNATIONAL   (United Kingdom)

Author keywords

Anti nematode; Biopesticide; Cystatin; OcI D86; Toxicity

Indexed keywords

CYSTATIN; PAPAIN; PHYTOCYSTATIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CECUM; CONTROLLED STUDY; CROP; DEGRADATION; DIET; GENE EXPRESSION REGULATION; HISTOCHEMISTRY; LIVER WEIGHT; MALE; NEMATODE; NONHUMAN; ORGAN WEIGHT; PEST RESISTANCE; PLANT PEST; POTATO; PROMOTER REGION; PROTEIN ENGINEERING; RAT; RICE; RISK ASSESSMENT; STOMACH JUICE; TOXICITY TESTING; TRANSGENIC PLANT;

ANIMALIA; NEMATODA; SOLANUM TUBEROSUM;

EID: 0942279592     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/134.2.431     Document Type: Article

References (25)

1. Environmental Protection Agency (2000) Bt Plant-Pesticides Registration Action Document http://www.epa.gov/scipoly/sap/2000/october/brad5_benefits_com.pdf (accessed 10/8/03).
2. Environmental Protection Agency (2000) Biopesticides Registration Action Document Preliminary Risks and Benefits Sections. Bacillus thuringiensis Plant-Pesticides: U. S. Environmental Protection Agency http://www.epa.gov/scipoly/sap/2000/october/brad1_execsum_overview.pdf (accessed 10/8/03).
3. Betz, F. S., Hammond, B. G. & Fuchs, R. L. (2000) Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regul. Toxicol. Pharmacol. 32: 156-173.
4. Gustafson, D. I. (1993) Pesticides in Drinking Water. Van Nostrand Reinhold, New York, NY.
5. Urwin, P. E., Atkinson, H. J., Waller, D. A. & McPherson, M. J. (1995) Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant J. 8: 121-131.
6. Urwin, P. E., Troth, K. M., Zubko, E. I. & Atkinson, H. J. (2001) Effective transgenic resistance to Globodera pallida in potato field trials. Mol. Breed. 8: 95-101.
7. Urwin, P. E., Jayne Green, J. & Atkinson, H. J. (2003) Resistance to Globodera spp conferred by a plant cystatin alone and enhancement by a cystatin pyramided with natural resistance. Mol. Breed. 12: 263-269.
8. Cowgill, S. E., Wright, C. & Atkinson, H. J. (2002a) Transgenic potatoes with enhanced levels of nematode resistance do not have altered susceptibility to nontarget aphids. Mol. Ecol. 11: 821-827.
9. Cowgill, S. E., Bardgett, R. D., Kiezebrink, D. T. & Atkinson, H. J. (2002b) The effect of transgenic nematode resistance on non-target organisms in the potato rhizosphere. J. Appl. Ecol. 39: 915-923.
10. Lilley, C. J., Urwin, P. E., Johnston, K. A. & Atkinson, H. J. (2003) Preferential expression of a plant cystatin at nematode feeding sites confers resistance to Meloidogyne and Globodera spp. Plant Biotechnol. J. (in press).
11. Richardson, M. (1991) Seed storage proteins: the enzyme inhibitors. Methods Plant Biochem. 5: 259-305.
12. Rodis, P. & Hoff, J. E. (1984) Naturally occurring protein crystals in the potato. Plant Physiol. 74: 907-911.
13. Awade A. C. (1996) On hen egg fractionation: applications of liquid chromatography to the isolation and the purification of hen egg white and egg yolk proteins. Z. Lebensm.-Unters-Forsch. 202: 1-14.
14. Veerman E.C.I., vandenKeybus, P.A.M., Vissink, A. & Amerongen A.V.N. (1996) Human glandular salivas: their separate collection and analysis. Eur. J. Oral Sci. 104: 346-352.
15. Pusztal, A., Grant, G., Brown, D. J., Stewart, C. & Bardocz, S. (1992) Nutritional evaluation of the trypsin (EC 3.4.21.4) inhibitor from cowpea (Vigna unguiculata Walp.). Br. J. Nutr. 68: 783-791.
16. Kuiper, H. A., Kleter, G. A., Noteborn, H.P.J.M. & Kok, E. J. (2001) Assessment of the food safety issues related to genetically modified foods. Plant J. 27: 503-528.
17. Harlow, E. & Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Astwood J. D., Leach J. N., Fuchs R. L. (1996) Stability of food allergens to digestion in vitro. Nat. Biotechnol. 14: 1269-1273.
19. Snedecor G. W. & Cochran W. G. (1989) Statistical Methods, 8th ed. Iowa State University Press, Ames, Iowa.
20. Abe K., Kondo, H. & Arai, S. (1987) Purification and characterization of a rice cysteine proteinase inhibitor. Agric. Biol. Chem. 51: 2763-2768.
21. Lawrence, J. C. & Nielsen S. S. (2001) Partial isolation and characterization of a cysteine proteinase inhibitor from lima bean (Phaseolus lunatus). J. Agric. Food Chem. 49: 1020-1025.
22. Boucoiran C. F. S., Kijne, J. W. & Recourt, K. (2000) Isolation and partial characterization of thermostable isoperoxidases from potato (Solanum tuberosum L.) tuber sprouts. Agric. Biol. Chem. 48: 701-707.
23. Fu, T.-J. (2002) Digestion stability as a criterion for protein allergenicity assessment. Ann. N.Y. Acad. Sci. 964: 99-110.
24. Madonado, L. (1998) CIP Potato Facts, Potato Indicators http://www.cipotato.org/market/potatofacts/potind.htm (accessed 10/8/03).
25. Environmental Protection Agency (1998) Dietary Exposure Analysis for Methidathion in Support of the Reregistration Eligibility Decision; http://www. epa.gov/pesticides/op/methidathion/methidathion_14.pdf (accessed 10/8/03).