Expression of a plant cystatin confers partial resistance to Globodera, full resistance is achieved by pyramiding a cystatin with natural resistance

Molecular Breeding

Volumn 12, Issue 3, 2003, Pages 263-269

  Urwin, Peter E ^a   Green, Jayne ^a   Atkinson, Howard J ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Globodera pallida; Natural; Nematode; Potato; Proteinase inhibitor; Resistance; Transgenic

Indexed keywords

PLANTS (BOTANY);

CHICKEN EGG WHITE; CYSTATINS; GLOBODERUM PALLIDA; NATURAL; NEMATODE; PALLIDA; POTATO; PROTEINASE INHIBITORS; RESISTANCE; TRANSGENICS;

AMINO ACIDS;

EUKARYOTA; GALLUS GALLUS; GLOBODERA; GLOBODERA PALLIDA; GLOBODERA ROSTOCHIENSIS; HELIANTHUS; SOLANUM TUBEROSUM;

EID: 0345015413     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026352620308     Document Type: Article

References (29)

1. Atkinson H.J. 2002. Molecular Approaches to Novel Crop Resistance Against Nematodes. In: Lee D.L. (ed.), The Biology of Nematodes, Taylor and Francis, London, UK. pp. 569-598.
2. Atkinson H.J., Green J., Cowgill S. and Levesley A. 2001. The case for genetically modified crops with a poverty focus. Trends Biotechnol. 19: 91-96.
3. Atkinson H.J., Urwin P.E., Lilley C.J. and McPherson M.J. 1998. Engineered resistance to plant nematodes. In: Perry R.N. and Wright D.J. (eds), The Physiology and Biochemistry of free-living and plant parasitic nematodes. CABI St Albans, UK pp. 381-405.
4. Dale M.F.B. and De Scurrah M.M. 1998. Breeding for resistance to the potato cyst nematodes Globodera rostochiensis and G. pallida: stratergies, mechanisms and genetic resources. In: Marks and Brodie (eds), Potatao Cyst Nematodes, Biology, Distrubution and Control. Wallingford CAB publishing, Oxon, UK. pp167-195.
5. Dellaporta S.L., Wood J. and Hicks J.B. 1983. A plant DNA minipreparation: version II. Plant Mol. Biol. Reporter 4: 19-21.
6. Dietze J., Blau A. and Willmitzer L. 1995. Agrobacterium-mediated transformation of potato (Solanum tuberosum). In: Potrykus I., Spangenberg G. (eds), Gene transfer to plants. Springer Lab manual. pp. 24-30.
7. Douches D.S., Kisha T.J., Coombs J.J., Li W., Pett W.L. and Grafius E.J. 2001. Effectiveness of natural and engineered host plant resistance in potato to the Colorado potato beetle. Hortsci. 36: 967-970.
8. Gubba A., Gonsalves C., Stevens M.R., Tricoli D.M., Gonsalves D. 2002. Combining transgenic and natural resistance to obtain broad resistance to tospovirus infection in tomato (Lycopersicon esculentum mill). Mol. Breeding 9: 13-23.
9. Haydock P.P.J. and Evans K. 1998. Management of potato cyst nematodes in the UK: an integrated approach? Outlook on Agriculture 27: 253-260.
10. Ho S.N., Hunt H.D., Horton R.M., Pullen J.K. and Pease L.R. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59.
11. Horton R.M., Hunt H.D., Ho S.N., Pullen J.K. and Pease L.R. 1989. Engineering hybrid genes without the use of restriction enzymes - gene splicing by overlap extension. Gene 77: 61-68.
12. Jach G., Gornhardt B., Mundy J., Logemann J., Pinsdorf E., Leah R., Schell J. and Maas C. 1995. Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J. 8: 97-109.
13. Koes R.E., Spelt C.E., Mol J.N.M. and Gerats A.G.M. 1987. The chalcone synthase multigene family of Petunia hybrida (V30): sequence homology, chromosomal localisation and evolutionary aspects. Plant Mol. Biol. 10: 159-169.
14. Liddell J.E., Cryer E. 1991. A practical guide to monoclonal antibodies. John Wiley and Sons, New York, USA pp 188.
15. Logemann J., Schell J. and Willmitzer L. 1987. Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163: 16-20.
16. Maqbool S.B., Riazuddin S., Loc N.T., Gatehouse A.M.R., Gatehouse J.A. and Christou P. 2001. Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol. Breeding 7: 85-93.
17. Minnis S.T., Haydock P.P.J., Ibrahim S.K., Grove I.G., Evans K. and Russell M.D. 2002. Potato cyst nematodes in England and Wales - occurrence and distribution. Annals App. Biol. 140: 187-195.
18. Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-497.
19. Powers T.O., Todd T.C., Burnell A.M., Murray P.C.B., Fleming C.C., Szalanski A.L., Adams B.A. and Harris T.S. 1997. The rDNA internal transcribed spacer region as a taxonomic marker for nematodes. J. Nematol. 29: 441-450.
20. Roberts P.A. 1992. Current Status of the availability, development and use of host plant resistance to nematodes. J. Nematology 24: 213-227.
21. Southey J.F. 1986. Laboratory methods for work with plant and soil nematodes. pp. 32-33. HMSO, London, UK.
22. Stancombe P. 1997. Molecular Diagnosis of potato cyst nematodes Thesis (Ph.D), University of Leeds
23. Tzortzakakis E.A. and Gowen S.R. 1996. Occurrence of a resistance breaking pathotype of Meloidogyne javanica on tomatoes in Crete, Greece. Fund. App. Nematol. 19: 283-288.
24. Urwin P.E. Atkinson H.J. Waller D.A. and McPherson M.J. 1995. Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera-pallida. Plant J. 8: 121-131.
25. Urwin P.E., Levesley A., McPherson M.J., Atkinson H.J. 2000. Transgenic resistance to the nematode Rotylenchulus reniformis conferred by Arabidopsis thaliana plants expressing proteinase inhibitors. Mol. Breeding 6: 257-264.
26. Urwin P.E., Lilley C.J., McPherson M.J. and Atkinson H.J. 1997a. Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin. Plant J. 12: 455-461.
27. Urwin P.E., Troth K.M., Zubko E.I. and Atkinson H.J. 2001. Effective transgenic resistance to Globodera pallida in potato field trials. Mol. Breeding 8: 95-101.
28. Urwin, P.E., McPherson M.J. and Atkinson H.J. 1998. Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs. Planta 204: 472-479.
29. Veremis J.C. and Roberts P.A. 1996. Relationships between Meloidogyne incognita resistance genes in Lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence. Theor. App. Genetics 93: 950-956.