An extra large insertion in the polyhydroxyalkanoate synthase from Delftia acidovorans DS-17: Its deletion effects and relation to cellular proteolysis

FEMS Microbiology Letters

Volumn 231, Issue 1, 2004, Pages 77-83

  Tsuge, Takeharu ^a   Imazu, Shin Ichi ^a   Takase, Kazuma ^b   Taguchi, Seiichi ^b,c   Doi, Yoshiharu ^a,b  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b RIKEN   (Japan)

^c MEIJI UNIVERSITY   (Japan)

Author keywords

Granule associated protein; Polyhydroxyalkanoate; Polyhydroxyalkanoate synthase; Proteolysis; hydrolase fold

Indexed keywords

BACTERIAL ENZYME; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DELFTIA ACIDOVORANS; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE DELETION; GENE INSERTION SEQUENCE; GENE MUTATION; GENETIC CODE; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL;

COMAMONAS; COMAMONAS ACIDOVORANS; DELFTIA ACIDOVORANS; ESCHERICHIA COLI;

EID: 0842330739     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00930-3     Document Type: Article

References (21)

1. Steinbüchel A. Perspectives for biotechnological production and utilization of biopolymers: Metabolic engineering of polyhydroxyalkanoate biosynthesis pathways as a successful example. Macromol. Biosci. 1:2001;1-24.
2. Sudesh K., Abe H., Doi Y. Synthesis, structure and properties of polyhydroxyalkanoate: Biological polyesters. Prog. Polym. Sci. 25:2000;1503-1555.
3. Tsuge T. Metabolic improvements and use of inexpensive carbon sources in microbial production of polyhydroxyalkanoate. J. Biosci. Bioeng. 94:2002;579-584.
4. Akiyama M., Tsuge T., Doi Y. Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermantation. Polym. Degrad. Stab. 80:2003;183-194.
5. Saito Y., Doi Y. Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans. Int. J. Biol. Macromol. 16:1994;99-104.
6. Sudesh K., Fukui T., Doi Y. Genetic analysis of Comamonas acidovorans polyhydroxyalkanoate synthase and factors affecting the incorporation of 4-hydroxybutyrate monomer. Appl. Environ. Microbiol. 64:1998;3437-3443.
7. Takase K., Taguchi S., Doi Y. Enhanced synthesis of poly(3- hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis and in vitro recombination of type II polyhydroxyalkanoate synthase gene. J. Biochem. (Tokyo). 133:2003;139-145.
8. Imai Y., Matsushima Y., Sugimura T., Terada M. A simple and rapid method for generating a deletion by PCR. Nucleic Acids Res. 19:1991;2785.
9. Karr D.B., Waters J.K., Emerich D.W. Analysis of poly-β- hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Appl. Environ. Microbiol. 46:1983;1339-1344.
10. Taguchi S., Maehara A., Takase K., Nakahara M., Nakamura H., Doi Y. Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system. FEMS Microbiol. Lett. 198:2001;65-71.
11. Kato M., Bao H.J., Kang C.K., Fukui T., Doi Y. Production of a novel copolymer of 3-hydroxybutyric acids and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugars. Appl. Microbiol. Biotechnol. 45:1996;363-370.
12. Abe H., Doi Y., Fukushima T., Eya H. Biosynthesis from gluconate of a random copolyester consisting of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. 61-3. Int. J. Biol. Macromol. 16:1994;115-119.
13. Gerngross T.U., Snell K.D., Peoples O.P., Sinskey A.J., Csuhai E., Masamune S., Stubbe J. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry. 33:1994;9311-9320.
14. Matsumoto K., Matsusaki H., Taguchi K., Seki M., Doi Y. Isolation and characterization of polyhydroxyalkanoates inclusions and their associated proteins in Pseudomonas sp. 61-3. Biomacromolecules. 3:2002;787-792.
15. Ollis D.L., Cheah E., Cygler M., Dijkstra B., Frolow F., Franken S.M., Harel M., Remington S.J., Silman I., Schrag J. The α/β hydrolase fold. Protein Eng. 5:1992;197-211.
16. Wieczorek R., Pries A., Steinbüchel A., Mayer F. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J. Bacteriol. 177:1995;2425-2435.
17. Rehm, B.H. and Steinbüchel, A. (2002) PHA synthase: the key enzymes of PHA synthesis. In: Biopolymers 3a (Doi, Y. and Steinbüchel, A., Eds.), pp. 173-215. Wiley-VCH, Weinheim.
18. Rehm B.H., Antonio R.V., Spiekermann P., Amara A.A., Steinbüchel A. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: In vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. Biochim. Biophys. Acta. 1594:2002;178-190.
19. Sudesh K., Fukui T., Taguchi K., Iwata T., Doi Y. Improved production of poly(4-hydroxybutyrate) by Comamonas acidovorans and its freeze-fracture morphology. Int. J. Biol. Macromol. 25:1999;79-85.
20. Muh U., Sinskey A.J., Kirby D.P., Lane W.S., Stubbe J. PHA synthase from Chromatium vinosum: Cysteine 149 is involved in covalent catalysis. Biochemistry. 38:1999;826-837.
21. Hezayen F.F., Steinbüchel A., Rehm B.H. Biochemical and enzymological properties of the polyhydroxybutyrate synthase from the extremely halophilic archaeon strain 56. Arch. Biochem. Biophys. 403:2002;284-291.