Aspartate 338 contributes to the cationic specificity and to driver-amino acid coupling in the insect cotransporter KAAT1

Cellular and Molecular Life Sciences

Volumn 61, Issue 2, 2004, Pages 243-256

  Mari, S A ^a   Soragna, A ^b   Castagna, M ^a   Bossi, E ^b   Peres, A ^b   Sacchi, V F ^a  

^a Institute of General Physiology and Biological Chemistry G Esposito   (Italy)

^b UNIVERSITY OF INSUBRIA   (Italy)

Author keywords

Amino acid transport; Ion selectivity; KAAT1; Manduca sexta; Structure function

Indexed keywords

4 AMINOBUTYRIC ACID; AMINO ACID DERIVATIVE; ASPARTIC ACID; CATION; ISOPROTEIN; SODIUM POTASSIUM CHLORIDE COTRANSPORTER;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; FEMALE; INSECT; LEPIDOPTERA; MAMMAL; MUTATION; NONHUMAN; PREDICTION; PROTEIN ANALYSIS; PROTEIN FAMILY; PROTEIN FUNCTION; STEREOCHEMISTRY;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; ANIMALS; ASPARTIC ACID; CARRIER PROTEINS; CELL MEMBRANE; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; INSECT PROTEINS; LEUCINE; MANDUCA; MEMBRANE GLYCOPROTEINS; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; ORGANIC ANION TRANSPORTERS; PATCH-CLAMP TECHNIQUES;

ANIMALIA; INSECTA; LEPIDOPTERA; MAMMALIA; MANDUCA SEXTA;

EID: 0842309133     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00018-003-3367-2     Document Type: Article

References (43)

1. Castagna M., Shayakul C., Trotti D., Sacchi V. F., Harvey W. R. and Hediger M. A. (1998) Cloning and characterization of a potassium-coupled amino acid transporter. Proc. Natl. Acad. Sci. USA 95: 5395-5400
2. Sacchi V. F. and Wolfersberger M. G. (1996) Amino acid absorption. In: Biology of the Insect Midgut, pp. 236-265, Lehane M. J. and Billingsley P. F. (eds), London. Chapman & Hall
3. - neurotransmitter transporters. J. Neurochem. 71: 1785-1803
4. --independent. J. Biol. Chem. 275: 24518-24526
5. + amino acid transporter KAAT1 mutant Y147F has increased transport activity and altered substrate selectivity. J. Exp. Biol. 206: 245-254
6. +/glucose transporter. I. Voltage-clamp studies. J. Membr. Biol. 125: 49-62
7. Wadiche J. I. and Kavanaugr M. P. (1998) Macroscopic and microscopic properties of a cloned glutamate transporter/chloride channel. J. Neurosci. 18: 7650-7661
8. i-2). J. Gen Physiol. 112: 1-18
9. Bossi E., Centinaio E., Castagna M., Giovannardi S., Vincenti S., Sacchi VF et al. (1999) Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes. J. Physiol. 515: 729-742
10. +-coupled oligopeptide cotransporter PepT1. J. Biol. Chem. 272: 7777-7785
11. Mager S., Kleinberger-Doron N., Keshet G. I., Davidson N., Kanner B. I. and Lester H. A. (1996) Ion binding and permeation at the GABA transporter GAT1. J. Neurosci. 16: 5405-5414
12. Forlani G., Bossi E., Perego C., Giovannardi S. and Peres A. (2001) Three kinds of currents in the canine betaine/GABA transporter BGT-1 expressed in Xenopus laevis oocytes. Biochim. Biophys. Acta Mol. Cell Res. 1538: 172-180
13. Guastella J., Nelson N., Nelson H., Czyzyc L., Keynan S., Miedel M. C. et al. (1990) Cloning and expression of a rat brain GABA transporter. Science 249: 1303-1306
14. Hofman B. J., Mezey E. and Brownstein M. J. (1991) Cloning of a serotonin transporter affected by antidepressants. Science 254: 579-580
15. Giros B., Mestikawy S., Godinot N., Zheng K., Han H., Yang-Feng T. et al. (1992) Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter. Mol. Pharmacol. 42: 383-390
16. Bossi E., Sacchi V. F. and Peres A. (1999) Ionic selectivity of the coupled and uncoupled currents carried by the amino acid transporter KAAT1. Pflugers Arch. 438: 788-796
17. Bossi E., Vincenti S., Sacchi V. F. and Peres A. (2000) Simultaneous measurements of ionic currents and leucine uptake at the amino acid cotransporter KAAT1 expressed in Xenopus laevis oocytes. Biochim.Biophys. Acta Mol. Cell Res. 1495: 34-39
18. Sacchi V. F., Castagna M., Mari S. A., Perego C., Bossi E. and Peres A. (2003) Glutamate 59 is critical for transport function of the amino acid cotransporter KAAT1. Am. J. Physiol. Cell Physiol. 285: C623-C632
19. Kanner B. I. (2003) Transmembrane domain I of the γ-aminobutyric acid transporter GAT-1 plays a crucial role in the transition between cation leak and transport modes. J. Biol. Chem. 278: 3705-3712
20. Harvey W. R., Wood J. L., Quatrale R. P. and Jungreis A. M. (1975) Cation distributions across the larval and pupal midgut of the Lepidopteran, Hyalophora cecropia, in vivo. J. Exp. Biol. 63: 321-330
21. Giordana B. and Sacchi V. F. (1978) Cellular ionic concentrations in the midgut of two larvae of Lepidoptera in vivo and in vitro. Comp. Biochem. Physiol. 59A: 17-20
22. + an alternate substrate? J. Exp. Biol. 162: 281-294
23. Petersen C. I. and DeFelice L. J. (1999) Ionic interactions in the Drosophila serotonin transporter identify it as a serotonin channel. Nat. Neurosci. 2: 635-610
24. Ni Y. G., Chen J.-G., Androustellis-Theotokis A., Huang C.-J., Moczydlowski E. and Rudnick G. (2001) A lithium-induced conformational change in serotonin transporter alters cocaine binding, ion conductance and reactivity of cys-109. J. Biol. Chem. 276: 30942-30947
25. Kavanaugh M. P., Bendahan A., Zerangue N., Zhang Y. and Kanner B. I. (1997) Mutation of an amino acid residue influencing potassium coupling in the glutamate transporter GLT-1 induces obligate exchange. J. Biol. Chem. 272: 1703-1708
26. -)-coupled γ-aminobutyric acid transporter from rat brain, impairs net flux but not exchange. J. Biol. Chem. 275: 34106-34113
27. Seal R. P., Shigeri Y., Eliasof S., Leighton B. H. and Amara S. G. (2001) Sulfhydryl modification of V449C in the glutamate transporter EAAT1 abolishes substrate transport but not the substrate-gated anion conductance. Proc. Natl. Acad. Sci. USA 98: 15324-15329
28. DeFelice L. J. and Blakeley R. D. (1996) Pore models for transporters? Biophys. J. 70: 579-580
29. Galzi J.-L. and Changeux J.-P. (1994) Neurotransmitter-gated ion channels as unconventional allosteric proteins. Curr. Opin. Struct. Biol. 4: 554-565
30. Mager S., Naeve J., Quick M., Labarca C., Davidson N. and Lester H. A. (1993) Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron 10: 177-188
31. Penado K. M. Y., Rudnick G. and Stephan M. M. (1998) Critical amino acid residues in transmembrane span 7 of the serotonin transporter identified by random mutagenesis. J. Biol. Chem. 273: 28098-28106
32. Kamdar G., Penado K. M. Y., Rudnick G. and Stephan M. M. (2001) Functional role of critical stripe residues in transmembrane span 7 of the serotonin transporter. J. Biol. Chem. 276: 4038-4045
33. Supplisson S. and Roux M. J. (2002) Why glycine transporters have different stoichiometries. FEBS Lett. 529: 93-101
34. Wadiche J. I., Arriza J. L., Amara S. G. and Kavanaugh M. P. (1995) Kinetics of a human glutamate transporter. Neuron 14: 1019-1027
35. Hille B. (2001) Ionic Channels of Excitable Membranes, 3. p. 722 Sinauer, Ass. Inc. (ed.) Sunderland, Mass.
36. Tusnady G. E. and Simon I. (2001) The HMMTOP transmembrane topology prediction server. Bioinformatics 9: 849-850
37. Krogh A., Larsson B., Heijne G. von and Sonnhammer E. L. L. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305: 567-580
38. Persson B. and Argos P. (1994) Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J. Mol. Biol. 237: 182-192
39. Persson B. and Argos P. (1996) Topology prediction of membrane proteins. Protein Sci. 5: 363-371
40. Hirokawa T., Boon-Chieng S. and Mitaku S. (1998) SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 4: 378-379
41. Jones D. T., Taylor W. R. and Thornton J. M. (1994) A model recognition approach to the prediction of all-helical membrane protein structure and topology. Biochemistry 33: 3038-3049
42. Pasquier C. and Hamodrakas S. J. (1999) An hierarchical artificial neural network system for the classification of transmembrane proteins. Protein Eng. 12: 631-634
43. Cserzo M., Wallin E., Simon I., Heijne G. von and Elofsson A. (1997) Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method. Protein Eng. 10: 673-676