Ionic interactions in the Drosophila serotonin transporter identify it as a serotonin channel

Nature Neuroscience

Volumn 2, Issue 7, 1999, Pages 605-610

  Petersen, Christina I ^a   DeFelice, Louis J ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ION CHANNEL; SEROTONIN; SEROTONIN TRANSPORTER;

ANIMAL CELL; ARTICLE; CLEARANCE; DROSOPHILA; MEMBRANE CHANNEL; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; SEROTONIN UPTAKE; VOLTAGE CLAMP; XENOPUS;

ANIMALS; CARRIER PROTEINS; DROSOPHILA; DROSOPHILA PROTEINS; FEMALE; LITHIUM; MEMBRANE GLYCOPROTEINS; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; NERVE TISSUE PROTEINS; OOCYTES; PAROXETINE; PATCH-CLAMP TECHNIQUES; RATS; SEROTONIN; SEROTONIN PLASMA MEMBRANE TRANSPORT PROTEINS; SODIUM; XENOPUS LAEVIS;

EID: 0033363222     PISSN: 10976256     EISSN: None     Source Type: Journal    
DOI: 10.1038/10158     Document Type: Article

References (37)

1. Guan, X. M. & McBride, W. J. Fluoxetine increases the extracellular levels of serotonin in the nucleus accumbens. Brain Res. Bull. 21, 43-46 (1998).
2. Fuller, R. W., Hemrick-Luecke, S. K. & Snoddy, H. D. Effects of duloxetine, an antidepressant drug candidate, on concentrations of monoamines and their metabolites in rats and mice. J. Pharmacol. Exp. Ther. 269, 132-136 (1994).
3. Barker, E. L. & Blakely, R. D. in Psychopharmacology: The Fourth Generation of Progress (eds. Bloom, F. E. & Kupfer, D. J.) 321-333 (Raven, New York, 1995).
4. Eccles, J. C. & Jaeger, J. C. The relationship between the mode of operation and the dimensions of the junctional regions at synapses and motor end-organs. Proc. R. Soc. Biol. Sci. 148, 38-56 (1958).
5. Clements, J. D., Lester, R. A., Tong, G., Jahr, C. E. & Westbrook, G. L. The time course of glutamate in the synaptic cleft. Science 258, 1498-1501 (1992).
6. Duncan, G. E. et al. Autoradiographic characterization of imipramine and citalopram binding in rat and human brain: species differences and relationships to serotonin innervation patterns. Brain Res. 591 181-197 (1992).
7. Bunin, M. A. & Wightman, R. M. Quantitative evaluation of 5-hydroxytryptamine (serotonin) neuronal release and uptake: an investigation of extrasynaptic transmission. J. Neurosci. 18, 4854-4860 (1998).
8. Mager, S. et al. Conducting states of a mammalian serotonin transporter. Neuron 12, 845-859 (1994).
9. Corey, J. L., Quick, M. W., Davidson, N., Lester, H. A. & Guastella, J. A cocaine-sensitive Drosophila serotonin transporter: Cloning, expression, and electrophysiological characterization. Proc. Natl. Acad. Sci. USA 91, 1188-1192 (1994).
10. Ramamoorthy, S. et al. Antidepressant- and cocaine-sensitive human serotonin transporter: molecular cloning, expression, and chromosomal localization. Proc. Natl. Acad. Sci. USA 90, 2542-2546 (1993).
11. Blakely, R. D. et al. Cloning and expression of a functional serotonin transporter from rat brain. Nature 354, 66-69 (1991).
12. Bruns, D., Engert, F. & Lux, H.-D. A fast activating presynaptic reuptake current during serotonergic transmission in identified neurons of Hirudo. Neuron 10, 559-572 (1993).
13. Galli, A., Petersen, C. I., deBlaquiere, M., Blakely, R. D. & DeFelice, L. J. Drosophila serotonin transporters have voltage-dependent uptake coupled to a serotonin-gated ion channel. J. Neurosci. 17, 3401-3411 (1997).
14. Humphreys, C. J., Levin, J. & Rudnick, G. Antidepressant binding to the porcine and human platelet serotonin transporters. Mol. Pharmacol. 33, 657-663 (1988).
15. Lin, F., Lester, H. A. & Mager, S. Single-channel currents produced by the serotonin transporter, and analysis of a mutation affecting ion permeation. Biophys. J. 71, 3126-3135 (1996).
16. Sonders, M. & Amara, S. G. Channels in transporters. Curr. Opin. Neurobiol. 6, 294-302 (1996).
17. DeFelice, L. J. & Blakely, R. D. Pore models for transporters? Biophys. J. 70, 579-580 (1996).
18. Sonders, M. S., Zhu, S.-J., Zahniser, N. R., Kavanaugh, M. P. & Amara, S. G. Multiple ionic conductances of the human dopamine transporter: the actions of dopamine and psychostimulants. J. Neurosci. 17, 960-974 (1997).
19. Cammack, J. N. & Schwartz, E. A. Channel behavior in a γ-aminobutyrate transporter. Proc. Natl. Acad. Sci. USA 93, 723-727 (1996).
20. Galli, A., Blakely, R. D. & DeFelice, L. J. Norepinephrine transporters have channel modes of conduction. Proc. Natl. Acad. Sci. USA 93, 8671-8676 (1996).
21. Hagiwara, S. & Takahashi, K. The anomalous rectification and cation selectivity of the membrane of a starfish egg cell. J. Membr. Biol. 18, 61-80 (1974).
22. Neher, E., Sandblom, J. & Eisenman, G. Ion selectivity, saturation, and block in gramicidin A channels. J. Membr. Biol. 40, 97-116 (1978).
23. Hess, P. & Tsien, R. W. Mechanism of ion permeation through calcium channels. Nature 309, 453-456 (1984).
24. Almers, W., McCleskey, E. W. & Palade, P. T. A non-selective cation conductance in frog muscle membrane blocked by micromolar external calcium ions. J. Physiol. (Lond.) 353, 565-583 (1984).
25. Almers, W. & McCleskey, E. W. Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore. J. Physiol. (Lond.) 353, 585-608 (1984).
26. Hille, B. Ionic Channels of Excitable Membranes 2nd edn. 362-389 (Sinauer, Sunderland, Massachusetts, 1992).
27. Aidley, D. J. & Stanfield, P. R. Ion channels 121-160 (Cambridge Univ. Press, Cambridge, UK, 1996).
28. Demchyshyn, L. L. et al. Cloning, expression, and localization of a chloride-facilitated, cocaine-sensitive serotonin transporter from Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 91, 5158-5162 (1994).
29. Trimmer, J. S. et al. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 3, 33-49 (1989).
30. + permeation and pH regulation at a mammalian serotonin transporter. J. Neurosci. 17, 2257-2266 (1997).
31. Keyes, S. R. & Rudnick, G. Coupling of transmembrane proton gradients to platelet serotonin transport. J. Biol. Chem. 257, 1172-1176 (1982).
32. Cool, D. R., Leibach, F. H. & Ganapathy, V. Modulation of serotonin uptake kinetics by ions and ion gradients in human placental brush-border membrane vesicles. Biochemistry 29 1818-1822 (1990).
33. Nelson, P. J. & Rudnick, G. Coupling between platelet 5-hydroxytryptamine and potassium transport. J. Biol. Chem. 254, 10084-10089 (1979).
34. Su, A., Mager, S., Mayo, S. L. & Lester, H. A. A multi-substrate single-file model for ion-coupled transporters. Biophys. J. 70, 762-777 (1996).
35. Gu, H., Wall, S. C. & Rudnick, G. Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence. J. Biol. Chem. 269, 7124-7130 (1994).
36. Hodgkin, A. L. & Keynes, R. D. The potassium permeability of a giant nerve fibre. J. Physiol. (Lond.) 128, 61-88 (1955).
37. Galli, A., Blakely, R. D. & DeFelice, L. J. Patch-clamp and amperometric recordings from norepinephrine transporters: channel activity and voltage-dependent uptake. (1999).