In Vitro and In Vivo Activities of a Triterpenoid Saponin Extract (PX-6518) from the Plant Maesa balansae against Visceral Leishmania Species

Antimicrobial Agents and Chemotherapy

Volumn 48, Issue 1, 2004, Pages 130-136

  Maes, Louis ^a   Vanden Berghe, Dirk ^a   Germonprez, Nils ^d   Quirijnen, Ludo ^b   Cos, Paul ^a   De Kimpe, Norbert ^c   Van Puyvelde, Luc ^d  

^a UNIVERSITY OF ANTWERP   (Belgium)

^b TIBOTEC BVBA   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

^d GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Viet Nam)

Author keywords

[No Author keywords available]

Indexed keywords

ALLOPURINOL; AMPHOTERICIN B; KETOCONAZOLE; METHANOL; PENTAMIDINE ISETHIONATE; PLANT EXTRACT; PX 6518; SAPONIN DERIVATIVE; STIBOGLUCONATE SODIUM; TRIFLURALIN; TRITERPENOID;

AMASTIGOTE; ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ANTIMICROBIAL ACTIVITY; ARTICLE; BODY WEIGHT; BONE MARROW; CELL DEATH; CELL LINE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DRUG EFFICACY; DRUG SCREENING; DRUG SENSITIVITY; FIBROBLAST; HEPATITIS; HOST CELL; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; IN VIVO STUDY; INTRACELLULAR SPACE; LEISHMANIA; LEISHMANIA DONOVANI; LEISHMANIASIS; LIVER; MACROPHAGE; MAESA BALANSEA; MALE; MEDICINAL PLANT; MOUSE; MOUSE STRAIN; NONHUMAN; PLANT LEAF; PRIORITY JOURNAL; SPECIES; SPLEEN; VIET NAM; VISCERA;

ANIMALS; ANTINEOPLASTIC AGENTS, PHYTOGENIC; ANTIPROTOZOAL AGENTS; CELL LINE, TUMOR; CELL SURVIVAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG SCREENING ASSAYS, ANTITUMOR; HUMANS; LEISHMANIA INFANTUM; LEISHMANIASIS, VISCERAL; MAGNETIC RESONANCE SPECTROSCOPY; MALE; METHANOL; MICE; MICE, INBRED BALB C; PEPTIDES; PLANT EXTRACTS; PLANT LEAVES; PLANTS, MEDICINAL; SAPONINS; SOLVENTS; VIETNAM;

EID: 0347992035     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.48.1.130-136.2004     Document Type: Article

References (41)

1. Akendengue, B., E. Ngou-Milama, A. Laurens, and R. Hocquemiller. 1999. Recent advances in the fight against leishmaniasis with natural products. Parasite 6:3-8.
2. Banduwardene, R., A. B. Mullen, and K. C. Carter. 1997. Immune responses of Leishmania donovani infected BALB/c mice following treatment with free and vesicular sodium stibogluconate formulations. Int. J. Immunopharmacol. 19:195-203.
3. Bantia, S., C. D. Parker, S. L. Ananth, L. L. Horn, K. Andries, P. Chand, P. L. Kotian, A. Dehghani, Y. El-Kattan, T. Lin, T. L. Hutchison, J. A. Montgomery, D. L. Kellog, and Y. S. Babu. 2001. Comparison of the anti-influenza virus activity of RWJ-270201 with those of oseltamivir and zanamivir. Antimicrob. Agents Chemother. 45:1162-1167.
4. Bradley, D. J. 1977. Regulation of Leishmania populations within the host. II. Genetic control of acute susceptibility of mice to Leishmania donovani infection. Clin. Exp. Immunol. 30:130-140.
5. Camacho, M. R., S. L. Croft, and J. D. Phillipson. 2000. Natural products as sources of antiprotozoal drugs. Curr. Opin. Anti-Infect. Investig. Drugs 2:47-62.
6. Chan, M. M., and D. Fong. 1990. Inhibition of leishmanias but not host macrophages by the antitubulin herbicide trifluralin. Science 249:924-926.
7. Collins, M., K. C. Carter, and A. J. Baillie. 1992. Visceral leishmaniasis in the BALB/c mouse: antimony tissue disposition and parasite suppression after the administration of free stibogluconate. Ann. Trop. Med. Parasitol. 86:35-40.
8. Croft, S. L., and V. Yardley. 2002. Chemotherapy of leishmaniasis. Curr. Pharm. Des. 8:319-342.
9. Delmas, F., C. Di Giorgio, R. Elias, M. Gasquet, N. Azas, V. Mshvildadze, G. Dekanosidze, E. Kemertelidze, and P. Timon-David. 2000. Antileishmanial activity of three saponins isolated from ivy, alpha-hederin, beta-hederin and hederacolchiside A1, as compared to their action on mammalian cells cultured in vitro. Planta Med. 66:343-347.
10. Desjeux, P. 2001. The increase in risk factors for leishmaniasis worldwide. Trans. R. Soc. Trop. Med. Hyg. 95:239-243.
11. Fournet, A., and V. Munoz. 2002. Natural products as trypanocidal, antileishmanial and antimalarial drugs. Curr. Top. Med. Chem. 2:1215-1237.
12. Germonprez, N., L. Van Puyvelde, L. Maes, M. Van Tri, and N. De Kimpe. New pentacyclic triterpene saponins with strong anti-leishmanial activity from the leaves of Maesa balansae. Tetrahedron, in press.
13. Guerin, P., J. P. Olliaro, S. Sundar, M. Boelaert, S. L. Croft, P. Desjeux, M. K. Wasunna, and A. D. Bryceson. 2002. Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda. Lancet Infect. Dis. 2:494-501.
14. Handman, E. 2001. Leishmaniasis: current status of vaccine development. Clin. Microbiol. Rev. 14:229-243.
15. Havens, C. G., N. Bryant, L. Asher, L. Lamoreaux, S. Perfetto, J. J. Brendle, and K. A. Werbovetz. 2000. Cellular effects of leishmanial tubulin inhibitors on L. donovani. Mol. Biochem. Parasitol. 110:223-236.
16. Hostettmann, K., and A. Marston. 1995. Chemistry and pharmacology of natural products. Saponins, p. 239-284. Cambridge University Press, Cambridge, United Kingdom.
17. Jiang, Z., J. F. Gallard, M. T. Adeline, V. Dumontet, M. V. Tri, T. Sevenet, and M. Pais. 1999. Six triterpenoid saponins from Maesa laxiflora. J. Nat. Prod. 62:873-876.
18. Jonckers, T. H., S. van Miert, K. Cimanga, C. Bailly, P. Colson, M. C. De Pauw-Gillet, H. van den Heuvel, M. Claeys, F. Lemiere, E. L. Esmans, J. Rozenski, L. Quirijnen, L. Maes, R. Dommisse, G. L. Lemiere, A. Vlietinck, and L. Pieters. 2002. Synthesis, cytotoxicity, and antiplasmodial and anti-trypanosomal activity of new neocryptolepine derivatives. J. Med. Chem. 45:3497-3508.
19. Koike, K., M. Kudo, Z. Jia, T. Nikaido, Y. Ide, and T. Sakura. 1999. New triterpenoid saponins from Maesa japonica. J. Nat. Prod. 62:228-232.
20. Koike, K., Z. Jia, and T. Nikaido. 2001. New triterpenoid saponins from Maesa tenera. Chem. Pharm. Bull. 49:758-761.
21. Legrand, P., A. Vertut-Doi, and J. Bolard. 1996. Comparative internalization and recycling of different amphotericin B formulations by a macrophage-like cell line. J. Antimicrob. Chemother. 37:519-533.
22. Maes, L. 2003. Lead identification and development of a new anti-leishmanial saponin PX-6518, isolated from the Vietnamese plant Maesa balansae. Ph.D. thesis. Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Antwerp University, Antwerp, Belgium.
23. Maes, L., N. Germonprez, L. Van Puyvelde, M. Van Tri, T. Ngoc Ninh, and N. De Kimpe. July 2000. Antiprotozoal saponins. International patent WO 00/38700.
24. Neal, R. A., and S. L. Croft. 1984. An in vitro system for determining the activity of compounds against the intracellular form of Leishmania donovani. J. Antimicrob. Chemother. 14:463-475.
25. O'Brien, J., I. Wilson, T. Orton, and F. Pognan. 2000. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267:5421-5426.
26. Okunji, C. O., M. M. Iwu, J. E. Jackson, and J. D. Tally. 1996. Biological activity of saponins from two Dracaena species. Adv. Exp. Med. Biol. 404: 415-428.
27. Pauwels, R., K. Andries, Z. Debyser, P. Van Daele, D. Schols, P. Stoffels, K. De Vreese, R. Woestenborghs, A. M. Vandamme, C. G. Janssen et al. 1993. Potent and highly selective human immunodeficiency virus type 1 (HIV-1) inhibition by a series of alpha-anilinophenyl acetamide derivatives targeted at HIV-1 reverse transcriptase. Proc. Natl. Acad. Sci. USA 90:1711-1715.
28. Pecoul, B., P. Chirac, P. Trouiller, and J. Pinel. 1999. Access to essential drugs in poor countries: a lost battle? JAMA 281:361-367.
29. Plock, A., W. Sokolowska-Kohler, and W. Presber. 2001. Application of flow cytometry and microscopical methods to characterize the effect of herbal drugs on Leishmania spp. Exp. Parasitol. 97:141-153.
30. Rex, J. H., M. A. Pfaller, T. J. Walsh, V. Chaturvedi, A. Espinel-Ingroff, M. A. Ghannoum, L. L. Gosey, F. C. Odds, M. G. Rinaldi, D. J. Sheehan, and D. W. Warnock. 2001. Antifungal susceptibility testing: practical aspects and current challenges. Clin. Microbiol. Rev. 14:643-658.
31. Ridoux, O., C. Di Giorgio, F. Delmas, R. Elias, V. Mshvildadze, G. Dekanosidze, E. Kemertelidze, G. Balansard, and P. Timon-David. 2001. In vitro antileishmanial activity of three saponins isolated from ivy, alpha-hederin, beta-hederin and hederacolchiside A(1), in association with pentamidine and amphotericin B. Phytother. Res. 15:298-301.
32. Roberts, W. L., J. D. Berman, and P. M. Rainey. 1995. In vitro antileishmanial properties of tri- and pentavalent antimonial preparations. Antimicrob. Agents Chemother. 39:1234-1239.
33. Romand, S., C. Della Bruna, R. Farinotti, and F. Derouin. 1996. In vitro and in vivo effects of rifabutin alone or combined with atovaquone against Toxoplasma gondii. Antimicrob. Agents Chemother. 40:2015-2020.
34. Sindambiwe, J. B., A. M. Balde, T. De Bruyne, L. Pieters, H. Van den Heuvel, M. Claeys, D. A. Vanden Berghe, and A. J. Vlietinck. 1996. Triterpenoid saponins from Maesa lanceolata. Phytochemistry 41:269-277.
35. Stauber, L. A. 1966. Characterisation of strains of Leishmania donovani. Exp. Parasitol. 18:1-11.
36. Sundar, S. 2001. Drug resistance in Indian visceral leishmaniasis. Trop. Med. Int. Health 6:849-854.
37. Sundar, S., T. K. Jha, C. P. Thakur, J. Engel, H. Sindermann, C. Fischer, K. Junge, A. Bryceson, and J. Berman. 2002. Oral miltefosine for Indian visceral leishmaniasis. N. Engl. J. Med. 347:1739-1746.
38. Taylor, M. A. 1990. A larval development test for the detection of anthelminthic resistance in nematodes of sheep. Res. Vet. Sci. 49:198-202.
39. Tuntiwachwuttikul, P., O. Pancharoen, W. Mahabusarakam, P. Wiriyachitra, W. C. Taylor, W. A. Bubb, and G. H. Towers. 1997. A triterpenoid saponin from Maesa ramentacea. Phytochemistry 44:491-495.
40. Vanden Berghe, D. A., and A. J. Vlietinck. 1991. Screening methods for antibacterial and antiviral agents from higher plants. Methods Plant Biochem. 6:47-69.
41. Yardley, V., and S. L. Croft. 2000. A comparison of the activities of three amphotericin B lipid formulations against experimental visceral and cutaneous leishmaniasis. Int. J. Antimicrob. Agents 13:243-248.