In vivo biotinylated recombinant antibodies: High efficiency of labelling and application to the cloning of active anti-human IgG1 Fab fragments

Journal of Immunological Methods

Volumn 224, Issue 1-2, 1999, Pages 129-140

  Sibler, Annie Paule ^a   Kempf, Emmanuelle ^a   Glacet, Arnaud ^b   Orfanoudakis, Georges ^a   Bourel, Dominique ^b   Weiss, Etienne ^a  

^a Lab d'Immunotechnologie M   (France)

^b Lab Francais Fractionnement B   (France)

Author keywords

Colony assay; Fab cloning; Human IgG1 detection; In vivo biotinylation; Recombinant conjugate

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANTIGEN SPECIFICITY; ARTICLE; COLONY FORMATION; HYBRIDOMA; IMMUNOGLOBULIN PRODUCTION; LABELING INDEX; MOLECULAR CLONING; MOUSE; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ACETYL-COA CARBOXYLASE; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BIOTIN; CARBON-NITROGEN LIGASES; CARRIER PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE;

EID: 0032983924     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00016-2     Document Type: Article

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