Restoration of the activity of active-site mutants of the hyperthermophilic β-glycosidase from Sulfolobus solfataricus: Dependence of the mechanism on the action of external nucleophiles

Biochemistry

Volumn 37, Issue 49, 1998, Pages 17262-17270

  Moracci, Marco ^a   Trincone, Antonio ^a   Perugino, Giuseppe ^a   Ciaramella, Maria ^a   Rossi, Mosé ^a,b  

^a CNR   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOSIDASE;

ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; SULFOLOBUS SOLFATARICUS;

BINDING SITES; CATALYTIC DOMAIN; ENZYME ACTIVATION; FORMIC ACIDS; GLUCOSIDASES; GLUCOSIDES; GLUTAMIC ACID; GLUTAMINE; GLYCINE; HEAT; KINETICS; MUTAGENESIS, SITE-DIRECTED; SODIUM AZIDE; SULFOLOBUS;

ARCHAEA; BACTERIA (MICROORGANISMS); SULFOLOBUS SOLFATARICUS;

EID: 0032497939     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981855f     Document Type: Article

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