High glucose concentration in isotonic media alters Caco-2 cell permeability

AAPS PharmSci

Volumn 5, Issue 3, 2003, Pages

  D'Souza, Vanessa M ^a   Shertzer, Howard G ^b   Menon, Anil G ^c   Pauletti, Giovanni M ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

^b UNIVERSITY OF CINCINNATI MEDICAL CENTER   (United States)

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Caco 2; Glucose; Membrane fluidity; Solute flux; TEER; Tight junctions

Indexed keywords

DIGOXIN; F ACTIN; GLUCOSE; GLYCOPROTEIN P; GLYCYLSARCOSINE; HYDROCORTISONE; ISOTONIC SOLUTION; MANNITOL; MEMBRANE LIPID; PEPTIDE TRANSPORTER 1; PROTEIN ZO1;

ACTIN FILAMENT; ACTIVE TRANSPORT; ARTICLE; CELL MEMBRANE FLUIDITY; CELL MEMBRANE PERMEABILITY; CELL STRAIN CACO 2; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; DIFFUSION; DRUG TRANSPORT; ELECTRIC RESISTANCE; HUMAN; HUMAN CELL; INTESTINE MUCOSA; MEMBRANE STRUCTURE; MEMBRANE TRANSPORT; MONOLAYER CULTURE; PROTEIN TRANSPORT; TRANSPORT KINETICS;

EID: 0346670288     PISSN: 15221060     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Lee CP, de Vrueh, RLA, Smith PL. Selection of development candidates based on in vitro permeability measurements. Adv Drug Deliv Rev. 1997;23:47-62.
2. Nielsen CU, Andersen R, Brodin B, Frokjaer S, Taub ME, Steffansen B. Dipeptide model prodrugs for the intestinal oligopeptide transporter: affinity for and transport via hPepT1 in the human intestinal Caco-2 cell line. J Control Release. 2001;76:129-138.
3. Friedrichsen GM, Jakobsen P, Taub M, Begtrup M. Application of enzymatically stable dipeptides for enhancement of intestinal permeability: synthesis and in vitro evaluation of dipeptide-coupled compounds. Bioorg Med Chem. 2001;9:2625-2632.
4. Kulkarni A, Han Y, Hopfinger AJ. Predicting Caco-2 cell permeation coefficients of organic molecules using membrane-interaction QSAR analysis. J Chem Inf Comput Sci. 2002;42:331-342.
5. Bohets H, Annaert P, Mannens G, et al. Strategies for absorption screening in drug discovery and development. Curr Top Med Chem. 2001;1:367-383.
6. Markowska M, Oberle R, Juzwin S, Hsu CP, Gryszkiewicz M, Streeter AJ. Optimizing Caco-2 cell monolayers to increase throughput in drug intestinal absorption analysis. J Pharmacol Toxicol Methods. 2001;46:51-55.
7. Liang E, Chessic K, Yazdanian M. Evaluation of an accelerated Caco-2 cell permeability model. J Pharm Sci. 2000;89:336-345.
8. Parrott N, Lave T. Prediction of intestinal absorption: comparative assessment of GASTROPLUS and IDEA. Eur J Pharm Sci. 2002;17:51-61.
9. Yamashita S, Konishi K, Yamazaki Y, et al. New and better protocols for a short-term Caco-2 cell culture system. J Pharm Sci. 2002;91:669-679.
10. Briske-Anderson MJ, Finley JW, Newman SM. The influence of culture time and passage number on the morphological and physiological development of Caco-2 cells. Proc Soc Exp Biol Med. 1997;214:248-257.
11. Bestwick CS, Milne L. Alteration of culture regime modifies antioxidant defenses independent of intracellular reactive oxygen levels and resistance to severe oxidative stress within confluent Caco-2 "intestinal cells." Dig Dis Sci. 2001;46:417-423.
12. Jumarie C, Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J Cell Physiol. 1991;149:24-33.
13. Hidalgo IJ, Raub TJ, Borchardt RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology. 1989;96:736-749.
14. Ha H, Lee HB. Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose. Kidney Int Suppl. 2000;77:S19-S25.
15. Clarkson MR, Murphy M, Gupta S, et al. High glucose-altered gene expression in mesangial cells: actin-regulatory protein gene expression is triggered by oxidative stress and cytoskeletal disassembly. J Biol Chem. 2002;277:9707-9712.
16. D'Souza VM, Buckley DJ, Buckley AR, Shertzer HG, Pauletti GM. Glucose-mediated regulation of the intestinal oligopeptide transporter (PepT-1) in Caco-2 cells. AAPS PharmSci. 2002;4. Abstract R6177.
17. D'Souza VM, Buckley DJ, Buckley AR, Pauletti GM. Extracellular glucose concentration alters functional activity of the intestinal oligopeptide transporter (PepT-1) in Caco-2 cells. J Pharm Sci. 2003;92:594-603.
18. Rao R, Baker RD, Baker SS. Inhibition of oxidant-induced barrier disruption and protein tyrosine phosphorylation in Caco-2 cell monolayers by epidermal growth factor. Biochem Pharmacol. 1999;57:685-695.
19. Banan A, Choudhary S, Zhang Y, Fields JZ, Keshavarzian A. Oxidant-induced intestinal barrier disruption and its prevention by growth factors in a human colonic cell line: role of the microtubule cytoskeleton. Free Radic Biol Med. 2000;28:727-738.
20. Jourd'heuil D, Meddings JB. Oxidative and drug-induced alterations in brush border membrane hemileaflet fluidity, functional consequences for glucose transport. Biochim Biophys Acta. 2001;1510:342-353.
21. Banan A, Fields JZ, Zhang Y, Keshavarzian A. iNOS upregulation mediates oxidant-induced disruption of F-actin and barrier of intestinal monolayers. Am J Physiol Gastrointest Liver Physiol. 2001;280:G1234-1246.
22. Wu SJ, Robinson JR. Transcellular and lipophilic complex-enhanced intestinal absorption of human growth hormone. Pharm Res. 1999;16:1266-1272.
23. Pauletti GM, Okumu FW, Borchardt RT. Effect of size and charge on the passive diffusion of peptides across Caco-2 cell monolayers via the paracellular pathway. Pharm Res. 1997;14:164-168.
24. Barthe L, Woodley J, Houin G. Gastrointestinal absorption of drugs: methods and studies. Fundam Clin Pharmacol. 1999;13:154-168.
25. Adson A, Raub TJ, Burton PS, et al. Quantitative approaches to delineate paracellular diffusion in cultured epithelial cell monolayers. J Pharm Sci. 1994;83:1529-1536.
26. Pade V, Stavchansky S. Estimation of the relative contribution of the transcellular and paracellular pathway to the transport of passively absorbed drugs in the Caco-2 cell culture model. Pharm Res. 1997;14:1210-1215.
27. Artursson P, Palm K, Luthman K. Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv Drug Deliv Rev. 2001;46:27-43.
28. Ren S, Lien EJ. Caco-2 cell permeability vs human gastrointestinal absorption: QSPR analysis. Prog Drug Res. 2000;54:1-23.
29. Madara JL, Moore R, Carlson S. Alteration of intestinal tight junction structure and permeability by cytoskeletal contraction. Am J Physiol. 1987;253:C854-861.
30. Tanaka Y, Taki Y, Sakane T, Nadai T, Sezaki H, Yamashita S. Characterization of drug transport through tight-junctional pathway in Caco-2 monolayer: comparison with isolated rat jejunum and colon. Pharm Res. 1995;12:523-528.
31. Pappenheimer JR. Physiological regulation of epithelial junctions in intestinal epithelia. Acta Physiol Scand Suppl. 1988;571:43-51.
32. Podolin DA, Sutherland E, Iwahashi M, Simon FR, Pagliassotti MJ. A high-sucrose diet alters the lipid composition and fluidity of liver sinusoidal membranes. Horm Metab Res. 1998;30:195-199.
33. Stephens RH, O'Neill CA, Warhurst A, Carlson GL, Rowland M, Warhurst G. Kinetic profiling of P-glycoprotein-mediated drug efflux in rat and human intestinal epithelia. J Pharmacol Exp Ther. 2001;296:584-591.