Non-thermal effects of power-line magnetic fields (50 Hz) on gene expression levels of pluripotent embryonic stem cells - The role of tumour suppressor p53

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 557, Issue 1, 2004, Pages 63-74

  Czyz, Jaroslaw ^a,c   Nikolova, Teodora ^a   Schuderer, Jürgen ^b   Kuster, Niels ^b   Wobus, Anna M ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b ETH ZURICH   (Switzerland)

^c JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Differentiation; Electromagnetic fields; Mouse embryonic stem cells; Non thermal effect; p53

Indexed keywords

BAG 1 PROTEIN; EARLY GROWTH RESPONSE FACTOR 1; HEAT SHOCK PROTEIN 70; MYC PROTEIN; PROTEIN C JUN; PROTEIN P21; PROTEIN P53; TUMOR SUPPRESSOR PROTEIN;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION; GENETIC TRANSCRIPTION; IN VITRO STUDY; MAGNETIC FIELD; MOUSE; NONHUMAN; ONCOGENE C MYC; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; REGULATOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; WILD TYPE;

MAMMALIA;

EID: 0346422368     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2003.09.011     Document Type: Article

References (71)

1. Wertheimer N., Leeper E. Electrical wiring configurations and childhood cancer. Am. J. Epidemiol. 109:1979;273-284.
2. Savitz D.A., Wachtel H., Barnes F.A., John E.M., Tvrdik J.G. Case-control study of childhood cancer and exposure to 60. Hz magnetic fields Am. J. Epidemiol. 128:1988;21-38.
3. Feychting M., Forssen U., Floderus B. Occupational and residential magnetic field exposure and leukemia and central nervous system tumors. Epidemiology. 8:1997;384-389.
4. Tenforde T.S. Biological interactions and potential health effects of extremely-low-frequency magnetic fields from power lines and other common sources. Annu. Rev. Public Health. 13:1992;173-196.
5. Moulder J.E., Foster K.R. Biological effects of power-frequency fields as they relate to carcinogenesis. Proc. Soc. Exp. Biol. Med. 209:1995;309-324.
6. Li C.Y., Theriault G., Lin R.S. Residential exposure to 60. Hz magnetic fields and adult cancers in Taiwan Epidemiology. 8:1997;25-30.
7. Hakansson N., Floderus B., Gustavsson P., Johansen C., Olsen J.H. Cancer incidence and magnetic field exposure in industries using resistance welding in Sweden. Occup. Environ. Med. 59:2002;481-486.
8. Bethwaite P., Cook A., Kennedy J., Pearce N. Acute leukemia in electrical workers: a New Zealand case-control study. Cancer Causes Control. 12:2001;683-689.
9. Michaelis J., Schuz J., Meinert R., Menger M., Grigat J.P., Kaatsch P., Kaletsch U., Miesner A., Stamm A., Brinkmann K., Karner H. Childhood leukemia and electromagnetic fields: results of a population-based case-control study in Germany. Cancer Causes Control. 8:1997;167-174.
10. Harris A.W., Basten A., Gebski V., Noonan D., Finnie J., Bath M.L., Bangay M.J., Repacholi M.H. A test of lymphoma induction by long-term exposure of E mu-Pim1 transgenic mice to 50. Hz magnetic fields Radiat. Res. 149:1998;300-307.
11. Verkasalo P.K., Pukkala E., Hongisto M.Y., Valjus J.E., Jarvinen P.J., Heikkila K.V., Koskenvuo M. Risk of cancer in Finnish children living close to power lines. BMJ. 307:1993;895-899.
12. Tomenius L. 50. Hz electromagnetic environment and the incidence of childhood tumors in Stockholm County Bioelectromagnetics. 7:1986;191-207.
13. Schreiber G.H., Swaen G.M., Meijers J.M., Slangen J.J., Sturmans F. Cancer mortality and residence near electricity transmission equipment: a retrospective cohort study. Int. J. Epidemiol. 22:1993;9-15.
14. Liburdy R.P. Calcium signaling in lymphocytes and ELF fields. Evidence for an electric field metric and a site of interaction involving the calcium ion channel. FEBS Lett. 301:1992;53-59.
15. Liburdy R.P. Biological interactions of cellular systems with time-varying magnetic fields. Ann. N. Y. Acad. Sci. 649:1992;74-95.
16. Pipkin J.L., Hinson W.G., Young J.F., Rowland K.L., Shaddock J.G., Tolleson W.H., Duffy P.H., Casciano D.A. Induction of stress proteins by electromagnetic fields in cultured HL-60 cells. Bioelectromagnetics. 20:1999;347-357.
17. Goodman R., Blank M. Insights into electromagnetic interaction mechanisms. J. Cell Physiol. 192:2002;16-22.
18. Woods M., Bobanovic F., Brown D., Alexander D.R. Lyn and syk tyrosine kinases are not activated in B-lineage lymphoid cells exposed to low-energy electromagnetic fields. FASEB J. 14:2000;2284-2290.
19. Dibirdik I., Kristupaitis D., Kurosaki T., Tuel-Ahlgren L., Chu A., Pond D., Tuong D., Luben R., Uckun F.M. Stimulation of Src family protein-tyrosine kinases as a proximal and mandatory step for SYK kinase-dependent phospholipase Cgamma2 activation in lymphoma B cells exposed to low energy electromagnetic fields. J. Biol. Chem. 273:1998;4035-4039.
20. Jin M., Blank M., Goodman R. ERK1/2 phosphorylation, induced by electromagnetic fields, diminishes during neoplastic transformation. J. Cell Biochem. 78:2000;371-379.
21. Wetzel B.J., Nindl G., Vesper D.N., Swez J.A., Jasti A.C., Johnson M.T. Electromagnetic field effects: changes in protein phosphorylation in the Jurkat E6.1 cell line. Biomed. Sci. Instrum. 37:2001;203-208.
22. Sisken B.F., Jacob J.M., Walker J.L. Acute treatment with pulsed electromagnetic fields and its effect on fast axonal transport in normal and regenerating nerve. J. Neurosci. Res. 42:1995;692-699.
23. Lin H., Goodman R., Shirley-Henderson A. Specific region of the c-myc promoter is responsive to electric and magnetic fields. J. Cell Biochem. 54:1994;281-288.
24. Phillips J.L., Haggren W., Thomas W.J., Ishida-Jones T., Adey W.R. Magnetic field-induced changes in specific gene transcription. Biochim. Biophys. Acta. 1132:1992;140-144.
25. Rao S., Henderson A.S. Regulation of c-fos is affected by electromagnetic fields. J. Cell Biochem. 63:1996;358-365.
26. Narita K., Hanakawa K., Kasahara T., Hisamitsu T., Asano K. Induction of apoptotic cell death in human leukemic cell line, HL-60, by extremely low frequency electric magnetic fields: analysis of the possible mechanisms in vitro. In Vivo. 11:1997;329-335.
27. Simko M., Kriehuber R., Weiss D.G., Luben R.A. Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines. Bioelectromagnetics. 19:1998;85-91.
28. Ismael S.J., Callera F., Garcia A.B., Baffa O., Falcao R.P. Increased dexamethasone-induced apoptosis of thymocytes from mice exposed to long-term extremely low frequency magnetic fields. Bioelectromagnetics. 19:1998;131-135.
29. Schimmelpfeng J., Dertinger H. Action of a 50 Hz magnetic field on proliferation of cells in culture. Bioelectromagnetics. 18:1997;177-183.
30. Ruiz Gomez M.J., Pastor Vega J.M., de la P.L., Gil C.L., Martinez M.M. Growth modification of human colon adenocarcinoma cells exposed to a low-frequency electromagnetic field. J. Physiol. Biochem. 55:1999;79-83.
31. Ivancsits S., Diem E., Pilger A., Rudiger H.W., Jahn O. Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts. Mutat. Res. 519:2002;1-13.
32. Ubeda A., Trillo M.A., Chacon L., Blanco M.J., Leal J. Chick embryo development can be irreversibly altered by early exposure to weak extremely-low-frequency magnetic fields. Bioelectromagnetics. 15:1994;385-398.
33. Di Carlo A.L., Litovitz T.A. Is genetics the unrecognized confounding factor in bioelectromagnetics? Flock-dependence of field-induced anoxia protection in chick embryos. Bioelectrochem. Bioenerg. 48:1999;209-215.
34. Wobus A.M., Guan K., Yang H.T., Boheler K.R. Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation. Methods Mol. Biol. 185:2002;127-156.
35. Spielmann H., Pohl I., Doring B., Liebsch M., Moldenhauer F. The embryonic stem cell test, an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. In Vitro Toxicol. 10:1997;119-127.
36. Brugarolas J., Moberg K., Boyd S.D., Taya Y., Jacks T., Lees J.A. Inhibition of cyclin-dependent kinase 2 by p21 is necessary for retinoblastoma protein-mediated G1 arrest after gamma-irradiation. Proc. Natl. Acad. Sci. U.S.A. 96:1999;1002-1007.
37. Sigal A., Rotter V. Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome. Cancer Res. 60:2000;6788-6793.
38. Bernstein C., Bernstein H., Payne C.M., Garewal H. DNA repair/pro-apoptotic dual-role proteins in five major DNA repair pathways: fail-safe protection against carcinogenesis. Mutat. Res. 511:2002;145-178.
39. Oren M. Decision making by p53: life, death and cancer. Cell Death. Differ. 10:2003;431-442.
40. J. Czyz, K. Guan, Q. Zeng, T. Nikolova, A. Meister, F. Schönborn, J. Schuderer, N. Kuster, A.M. Wobus, High frequency electromagnetic fields affect gene expression levels in tumor suppressor-deficient embryonic stem cells, Bioelectromagnetics (in press).
41. Doetschman T.C., Eistetter H., Katz M., Schmidt W., Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87:1985;27-45.
42. Jacks T., Remington L., Williams B.O., Schmitt E.M., Halachmi S., Bronson R.T., Weinberg R.A. Tumor spectrum analysis in p53-mutant mice. Curr. Biol. 4:1994;1-7.
43. International Electrotechnical Commission (IEC), Limitations of emission of harmonic currents for equipment connected to medium and high power supply systems', IEC 1000 Electromagnetic Compatibility. Part 3. Limits, Section 6, Technical Report, 1995.
44. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
45. Lamph W.W., Wamsley P., Sassone-Corsi P., Verma I.M. Induction of proto-oncogene JUN/AP-1 by serum and TPA. Nature. 334:1988;629-631.
46. Pal S.K., Crowell R., Kiessling A.A., Cooper G.M. Expression of proto-oncogenes in mouse eggs and preimplantation embryos. Mol. Reprod. Dev. 35:1993;8-15.
47. Miyazaki T. Two distinct steps during thymocyte maturation from CD4-CD8- to CD4+CD8+ distinguished in the early growth response (Egr)-1 transgenic mice with a recombinase-activating gene-deficient background. J. Exp. Med. 186:1997;877-885.
48. Loimas S., Wahlfors J., Janne J. Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos. Hum. Gene Ther. 8:1998;965-977.
49. Kuwano K., Hagimoto N., Nomoto Y., Kawasaki M., Kunitake R., Fujita M., Miyazaki H., Hara N. P53 and p21 (Waf1/Cip1) mRNA expression associated with DNA damage and repair in acute immune complex alveolitis in mice. Lab. Invest. 76:1997;161-169.
50. Hole N., Graham G.J., Menzel U., Ansell J.D. A limited temporal window for the derivation of multilineage repopulating hematopoietic progenitors during embryonal stem cell differentiation in vitro. Blood. 88:1996;1266-1276.
51. Rohwedel J., Guan K., Zuschratter W., Jin S., Ahnert-Hilger G., Furst D., Fassler R., Wobus A.M. Loss of beta1 integrin function results in a retardation of myogenic, but an acceleration of neuronal, differentiation of embryonic stem cells in vitro. Dev. Biol. 201:1998;167-184.
52. Murphy J.C., Kaden D.A., Warren J., Sivak A. Power frequency electric and magnetic fields: a review of genetic toxicology. Mutat. Res. 296:1993;221-240.
53. McCann J., Kavet R., Rafferty C.N. Assessing the potential carcinogenic activity of magnetic fields using animal models. Environ. Health Perspect. 108(Suppl. 1):2000;79-100.
54. McCann J., Kavet R., Rafferty C.N. Testing electromagnetic fields for potential carcinogenic activity: a critical review of animal models. Environ. Health Perspect. 105(Suppl. 1):1997;81-103.
55. Loscher W. Do cocarcinogenic effects of ELF electromagnetic fields require repeated long-term interaction with carcinogens? Characteristics of positive studies using the DMBA breast cancer model in rats. Bioelectromagnetics. 22:2001;603-614.
56. Juutilainen J., Lang S., Rytomaa T. Possible cocarcinogenic effects of ELF electromagnetic fields may require repeated long-term interaction with known carcinogenic factors. Bioelectromagnetics. 21:2000;122-128.
57. Zhang W., Chen S. EGR-1, a UV-inducible gene in p53(-/-) mouse cells. Exp. Cell Res. 266:2001;21-30.
58. Quinones A., Dobberstein K.U., Rainov N.G. The egr-1 gene is induced by DNA-damaging agents and non-genotoxic drugs in both normal and neoplastic human cells. Life Sci. 72:2003;2975-2992.
59. Hallahan D.E., Dunphy E., Virudachalam S., Sukhatme V.P., Kufe D.W., Weichselbaum R.R. c-jun and Egr-1 participate in DNA synthesis and cell survival in response to ionizing radiation exposure. J. Biol. Chem. 270:1995;30303-30309.
60. el Deiry W.S., Tokino T., Velculescu V.E., Levy D.B., Parsons R., Trent J.M., Lin D., Mercer W.E., Kinzler K.W., Vogelstein B. WAF1, a potential mediator of p53 tumor suppression. Cell. 75:1993;817-825.
61. de Belle I., Huang R.P., Fan Y., Liu C., Mercola D., Adamson E.D. p53 and Egr-1 additively suppress transformed growth in HT1080 cells but Egr-1 counteracts p53-dependent apoptosis. Oncogene. 18:1999;3633-3642.
62. Aladjem M.I., Spike B.T., Rodewald L.W., Hope T.J., Klemm M., Jaenisch R., Wahl G.M. ES cells do not activate p53-dependent stress responses and undergo p53-independent apoptosis in response to DNA damage. Curr. Biol. 8:1998;145-155.
63. Sabapathy K., Klemm M., Jaenisch R., Wagner E.F. Regulation of ES cell differentiation by functional and conformational modulation of p53. EMBO J. 16:1997;6217-6229.
64. Murphy L.O., Smith S., Chen R.H., Fingar D.C., Blenis J. Molecular interpretation of ERK signal duration by immediate early gene products. Nat. Cell Biol. 4:2002;556-564.
65. Valberg P.A., Kavet R., Rafferty C.N. Can low-level 50/60 Hz electric and magnetic fields cause biological effects? Radiat. Res. 148:1997;2-21.
66. Brocklehurst B., McLauchlan K.A. Free radical mechanism for the effects of environmental electromagnetic fields on biological systems. Int. J. Radiat. Biol. 69:1996;3-24.
67. Eveson R.W., Timmel C.R., Brocklehurst B., Hore P.J., McLauchlan K.A. The effects of weak magnetic fields on radical recombination reactions in micelles. Int. J. Radiat. Biol. 76:2000;1509-1522.
68. Blank M., Goodman R. Do electromagnetic fields interact directly with DNA? Bioelectromagnetics. 18:1997;111-115.
69. Panagopoulos D.J., Messini N., Karabarbounis A., Philippetis A.L., Margaritis L.H. A mechanism for action of oscillating electric fields on cells. Biochem. Biophys. Res. Commun. 272:2000;634-640.
70. Panagopoulos D.J., Karabarbounis A., Margaritis L.H. Mechanism for action of electromagnetic fields on cells. Biochem. Biophys. Res. Commun. 298:2002;95-102.
71. Binhi V.N., Goldman R.J. Ion-protein dissociation predicts 'windows' in electric field-induced wound-cell proliferation. Biochim. Biophys. Acta. 1474:2000;147-156.