Array analysis of gene expression in connexin-43 null astrocytes

Physiological Genomics

Volumn 15, Issue , 2004, Pages 177-190

  Iacobas, Dumitru A ^a   Urban Maldonado, Marcia ^a   Iacobas, Sanda ^a   Scemes, Eliana ^a   Spray, David C ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

DNA array; Gap junction; Glia; Intercellular communication; Knockout

Indexed keywords

CARRIER PROTEIN; CELL ADHESION MOLECULE; CELL CYCLE PROTEIN; CONNEXIN 43; GAP JUNCTION PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL COMMUNICATION; CELL GROWTH; CELL STRUCTURE; DNA HYBRIDIZATION; DNA MICROARRAY; DNA SEQUENCE; GAP JUNCTION; GENE DELETION; GENE EXPRESSION; GENETIC VARIABILITY; KNOCKOUT MOUSE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA EXTRACTION; SIGNAL TRANSDUCTION; UNINDEXED SEQUENCE; WILD TYPE;

ANIMALIA;

EID: 0346041563     PISSN: 15312267     EISSN: None     Source Type: Journal    
DOI: 10.1152/physiolgenomics.00062.2003     Document Type: Article

References (59)

1. Ai Z, Fischer A, Spray DC, Brown AMC, and Fishman GI. Wnt-1 regulation of Cx43 in cardiac myocytes. J Clin Invest 105: 161-171, 2000.
2. Alberts B, Bray D, Lewis J, Raff M, Roberts K, and Watson JD. Molecular Biology of the Cell (3rd ed.). New York: Garland, 1994.
3. Beyer EC, Paul DL, and Goodenough DA. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105: 2621-2629, 1987.
4. Britz-Cunningham SH, Shah MM, Zuppan CW, and Fletcher WH. Mutations of the connexin43 gap-junction gene in patients with heart malformations and defects of laterality. N Engl J Med 332: 1323-1329, 1995.
5. Butte A. The use and analysis of microarray data. Nat Rev Drug Discov 1: 951-960, 2002.
6. Carmody RJ, Hilliard B, Maguschak K, Chodosh LA, and Chen YH. Genomic scale profiling of autoimmune inflammation in the central nervous system: the nervous system response to inflammation. J Neuroimmunol 133: 95-107, 2002.
7. Dasgupta C, Martinex AM, Zuppan CW, Shah MM, Bailey LL, and Fletcher WH. Identification of connexin43 (alpha1) gap junction gene mutations in patients with hypoplastic left heart syndrome by denaturing gradient gel electrophoresis (DGGE). Mutat Res 479: 173-186, 2001.
8. Dermietzel R, Gao Y, Scemes E, Vieira D, Urban M, Kremer M, Bennett MVL, and Spray DC. Connexin43 null mice reveal that astrocytes express multiple connexins. Brain Res Rev 32: 45-56, 2000.
9. Dermietzel R, Hertzberg EL, Kessler JA, and Spray DC. Gap junctions between cultured astrocytes: immunocytochemical, molecular, and electrophysiological analysis. J Neurosci 11: 1421-1432, 1991.
10. Dermietzel R and Spray DC. Gap junctions in the brain: where, what type, how many and why? Trends Neurosci 16: 186-192, 1993.
11. Dermietzel R, Traub O, Hwang TK, Beyer E, Bennett MV, Spray DC, and Willecke K. Differential expression of three gap junction proteins in developing and mature brain tissues. Proc Natl Acad Sci USA 86: 10148-10152, 1989.
12. Dubina MV, Iatckii NA, Popov DE, Vasil'ev SV, and Krutovskikh V. Connexin43, but not connexin32, is mutated atadvanced stages of human sporadic colon cancer. Oncogene 21: 4992-4996, 2002.
13. Duffy HS, John GR, Lee SC, Brosnan CF, and Spray DC. Reciprocal regulation of the junctional proteins claudin-1 and connexin43 by interleukin-1 primary human fetal astrocytes. J Neurosci 20: RC114, 2000.
14. Duval N, Gomes D, Calaora V, Calabrese A, Meda P, and Bruzzone R. Cell coupling and Cx43 expression in embryonic mouse neural progenitor cells. J Cell Sci 115: 3241-3251, 2002.
15. Fushiki S, Perez Velazquez JL, Zhang L, Bechberger JF, Carlen PL, and Naus CC. Changes in neuronal migration in neocortex of connexin43 null mutant mice. J Neuropathol Exp Neurol 62: 304-314, 2003.
16. Goldberg GS, Bechberger JR, Tajima Y, Merritt M, Omori Y, Gawinowicz MA, Marayanan R, Tan Y, Sanai Y, Yamasaki H, Naus CC, Tsuda H, and Nicholson BJ. Connexin43 suppresses MFG-E8 while inducing contact growth inhibition of glioma cells. Cancer Res 60: 6018-6026, 2000.
17. Gutmann DH, Hedrick NM, Li J, Nagarajan R, Perry A, and Watson MA. Comparative gene expression profile analysis of neurofibromatosis 1-associated, and sporadic pilocytic astrocytomas. Cancer Res 62: 2085-2091, 2002.
18. Huang RP, Hossain MZ, Huang R, Gano J, Fan Y, and Boynton AL. Connexin43 (Cx43) enhances chemotherapy-induced apoptosis in human glioblastoma cells. Int J Cancer 92: 1301-1338, 2001.
19. Huang R, Lin Y, Wang CC, Gano J, Lin B, Shi Q, Boynton A, Burke J, and Huang RP. Connexin 43 suppresses human glioblastoma cell growth by down-regulation of monocyte chemotactic protein 1, as discovered using protein array technology. Cancer Res 62: 2806-2812, 2002.
20. Huang R, Liu YG, Lin Y, Fan Y, Boynton A, Yang D, and Huang RP. Enhanced apoptosis under low serum conditions in human glioblastoma cells by connexin 43 (Cx43). Mol Carcinog 32: 128-138, 2001.
21. Iacobas DA. Medical Biostatistics (3rd English ed.). Constanta, Romania: Tilia, 1997, p. 180-182.
22. Iacobas DA, Urban M, Iacobas S, and Spray DC. The "patholog" of the gene expression profile in evaluating the ecotoxin effects. Adv Ecol Sci 10: 733-742, 2001.
23. Iacobas DA, Iacobas S, and Spray DC. The "patholog" of the genes expression profile, a new tool in defining, evaluating, and classifying the genetic diseases. Rom J Physiol 37: 59-67, 2000.
24. Iacobas DA, Urban M, Massimi A, and Spray DC. Improved procedure to mine the spotted cDNA arrays. J Biomol Tech 13: 5-19, 2002.
25. Iacobas DA, Urban M, Massimi A, Iacobas S, and Spray DC. Hits and misses from gene expression ratio measurements in cDNA microarray studies. J Biomol Tech 13: 143-157, 2002.
26. Ideker T, Thorsson V, Siegel AF, and Hood LE. Testing for differentially-expressed genes by maximum-likelihood analysis of microarray data. J Comput Biol 7: 805-817, 2000.
27. Kostin S, Rieger M, Dammer S, Hein S, Richter M, Klovekorn WP, Bauer EP, and Schaper J. Gap junction remodeling and altered connexin43 expression in the failing human heart. Mol Cell Biochem 242: 135-144, 2003.
28. Li WE, Waldo K, Linask KL, Chen T, Wessels A, Parmacek MS, Kirby ML, and Lo CW. An essential role for connexin43 gap junctions in mouse coronary artery development. Development 129: 2031-2042, 2002.
29. Lin JH, Yang J, Liu S, Takano T, Wang X, Gao Q, Willecke K, and Nedergaard M. Connexin mediates gap junction-independent resistance to cellular injury. J Neurosci 23: 430-441, 2003.
30. Lin JH, Takano T, Cotrina ML, Arcuino G, Kang J, Liu S, Gao Q, Jiang L, Li F, Lichtenberg-Frate H, Haubrich S, Willecke K, Goldman SA, and Nedergaard M. Connexin 43 enhances the adhesivity and mediates the invasion of malignant glioma cells. J Neurosci 22: 4302-4311, 2002.
31. Liu XZ, Xia XJ, Adams J, Chen ZY, Welch KO, Tekin M, Ouyang XM, Kristiansen A, Pandya A, Balkany T, Arnos KS, and Nance WE. Mutations in GJA1 (connexin43) are associated with non-syndromic autosomal recessive deafness. Hum Mol Genet 10: 2945-2951, 2001.
32. Lock C, Hermans G, Pedotti R, Brendolan A, Schadt E, Garren H, Langer-Gould A, Strober S, Cannella B, Allard J, Klonowski P, Austin A, Lad N, Kaminski N, Galli SJ, Oksenberg JR, Raine CS, Heller R, and Steinman L. Gene-microarray analysis of multiple sclerosis lesions yields new targets validated in autoimmune encephalomyelitis. Nat Med 8: 500-508, 2002.
33. Loring JF, Wen X, Lee JM, Seilhamer J, and Somogyi R. A gene expression profile of Alzheimer's disease. DNA Cell Biol 20: 683-695, 2001.
34. Moorby C and Patel M. Dual functions for connexins: Cx43 regulates growth independently of gap junction formation. Exp Cell Res 271: 238-248, 2001.
35. Morley GE, Vaidya D, and Jalife J. Characterization of conductance in the ventricles of normal and heterozygous Cx43 knockout mice using optical imaging. J Cardiovasc Electrophysiol 11: 375-377, 2000.
36. Naus CC, Bechberger JF, Zhang Y, Venance L, Yamasaki H, Juneja SC, Kidder GM, and Giaume C. Altered gap junctional communication, intercellular signaling, and growth in cultured astrocytes deficient in connexin43. J Neurosci Res 49: 528-540, 1997.
37. Naus CC, Bond SL, Bechberger JF, and Rushlow W. Identification of genes differentially expressed in C6 glioma cells transfected with connexin 43. Brain Res Rev 32: 259-266, 2000.
38. Paznekas WA, Boyadjiev SA, Shapiro RE, Daniels O, Wollnik B, Keegan CE, Innis JW, Dinulos MB, Christian C, Hannibal MC, and Jabs EW. Connexin43 (GJA1) mutations cause the pleiotropic phenotype of oculodentodigital dysplasia. Am J Hum Genet 72: 408-418, 2003.
39. Perez-Velazquez JL, Frantseva M, Nauss CC, Bechberger JF, Juneja SC, Velumian A, Carlen PL, Kidder GM, and Mills LR. Development of astrocytes and neurons in cultured brain slices from mice lacking connexin43. Brain Res Dev Brain Res 97: 293-296, 1996.
40. Peters NS and Wit AL. Myocardial architecture and ventricular arrhythmogensis. Circulation 97: 1746-1754, 1998.
41. Plotkin LI, Manolagas C, and Bellido T. Transduction of cell survival signals by connexin43 hemichannels. J Biol Chem 277: 8648-8657, 2002.
42. Qin H, Sharo Q, Curtis H, Galipeau J, Belliveau DJ, Want T, Alaoui-Jamali MA, and Laird DW. Retroviral delivery of connexin genes to human breast tumor cells inhibits in vivo tumor growth by a mechanism that is independent of significant gap junctional intercellular communication. J Biol Chem 277: 29132-29138, 2002.
43. Rash JE, Yasumura T, Dudek FE, and Nagy JI. Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons. J Neurosci 21: 1983-2000, 2001.
44. Reaume AG, de Sousa PA, Kulkarni S, Langille BL, Zhu D, Davies TC, Juneja SC, Kidder GM, and Rossant J. Cardiac malformation in neonatal mice lacking connexin43. Science 267: 1831-1834, 1995.
45. Rozental R, Morales M, Mehler MF, Urban M, Kremer M, Dermietzel R, Kessler JA, and Spray DC. Changes in the properties of gap junctions during neuronal differentiation of hippocampal progenitor cells. J Neurosci 18: 1753-1762, 1998.
46. Rozental R, Srinivas M, Gokhan S, Urban M, Dermitzel R, Kessler JA, Spray DC, and Meltzer MF. Temporal expression of neuronal connexins during hippocampal ontogeny. Brain Res Rev 32: 57-71, 2000.
47. Scemes E, Suadicani SO, and Spray DC. Intercellular communication in spinal cord astrocytes: fine tuning between gap junctions and P2 nucleotide receptors in calcium wave propagation. J Neurosci 20: 1435-1445, 2000.
48. Splitt MP, Burn J, and Goodship J. Connexin43 mutations in sporadic and familial defects of laterality. N Engl J Med 333: 941, 1995.
49. Spray DC, Duffy HS, and Scemes E. Gap junctions in glia. Types, roles, and plasticity. Adv Exp Med Biol 468: 339-359, 1999.
50. Spray DC, Suadicani S, Srinivas M, Gutstein DE, and Fishman GI. Gap junctions in the cardiovascular system. Handbook of Physiology. The Cardiovascular System. The Heart. Bethesda, MD: Am Physiol Soc, 2001, sect. 2, vol. I, chapt. 4, p. 169-212.
51. Stains JP, Lecanda F, Screen J, Towler DA, and Civitelli R. Gap junctional communication modulates gene transcription by altering recruitment of Sp1 and SP3 to connexin-response elements in osteoblast promoters. J Biol Chem 278: 24377-24387, 2003.
52. Suadicani SO, DePina-Benabou M, Urban-Maldonado M, Spray DC, and Scemes E. Acute down-regulation of Cx43 alters P2Y receptor expression levels in mouse spinal cord astrocytes. Glia 42: 160-171, 2003.
53. Valdimarsson G and Kidder GM. Temporal control of gap junction assembly in preimplantation mouse embryos. J Cell Sci 108: 1715-1722, 1995.
54. Vis JC, Nicholson LF, Faull RL, Evans WH, Severs NJ, and Green CR. Connexin expression in Huntington's diseased human brain. Cell Bio Int 22: 837-847, 1998.
55. White TW and Paul DL. Genetic diseases and gene knockouts reveal diverse connexin functions. Annu Rev Physiol 61: 283-310, 1999.
56. Willecke K, Eiberger J, Degen J, Eckhardt D, Romualdi A, Guldenagel M, Duetsch U, and Sohl G. Structural and functional diversity of connexin genes in the mouse and human genome. Biol Chem 383: 725-737, 2002.
57. Xu X, Li WE, Huang GY, Meyer R, Chen T, Luo Y, Thomas MP, Radice GL, and Lo CW. Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions. J Cell Biol 154: 217-230, 2001.
58. Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, and Speed TP. Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res 30: e15, 2002.
59. Zhang YW, Morita I, Ikeda M, Ma KW, and Murota S. Connexin43 suppresses proliferation of osteosarcoma U2OS cells through post-transcriptional regulation of p27. Oncogene 20: 4138-4139, 2001.