Effect of cysteine mutations on direct electron transfer of horseradish peroxidase on gold

Biosensors and Bioelectronics

Volumn 17, Issue 11-12, 2002, Pages 953-963

  Ferapontova, Elena ^a   Schmengler, Kai ^b,c   Börchers, Torsten ^b   Ruzgas, Tautgirdas ^a   Gorton, Lo ^a  

^a LUND UNIVERSITY   (Sweden)

^b ICB Institut fur Chemo und Biosensorik GmbH   (Germany)

^c UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Bioelectrocatalysis; Gold electrodes; Heterogeneous direct electron transfer; P chip; Recombinant horseradish peroxidase

Indexed keywords

AMPEROMETRIC SENSORS; CATALYSIS; ENZYMES; ESCHERICHIA COLI; GOLD; MASS TRANSFER; PH EFFECTS; POLYPEPTIDES; REDUCTION;

DIRECT ELECTRON TRANSFER (ET); MEDIATORLESS DETECTION;

BIOELECTRIC PHENOMENA;

CATECHOL; CYSTEINE DERIVATIVE; GOLD; HISTIDINE; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; POLYPEPTIDE; PROTEIN; RECOMBINANT ENZYME; SILVER; SILVER CHLORIDE; STREPTAVIDIN; CYSTEINE; IMMOBILIZED ENZYME; RECOMBINANT PROTEIN;

ACCURACY; ADSORPTION; AMPEROMETRIC BIOSENSOR; AMPEROMETRY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL ANALYSIS; CHEMICAL MODIFICATION; COMPARATIVE STUDY; DEVICE; ELECTRIC CURRENT; ELECTROCHEMICAL ANALYSIS; ELECTROCHEMISTRY; ELECTRODE; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME IMMOBILIZATION; ENZYME STRUCTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE MUTATION; GENETIC ENGINEERING; OXIDATION; PH; PROTON TRANSPORT; REDUCTION; CHEMISTRY; CLASSIFICATION; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; EVALUATION; GENETIC PROCEDURES; GENETICS; INSTRUMENTATION; METHODOLOGY; PROTEIN ENGINEERING; SENSITIVITY AND SPECIFICITY; SITE DIRECTED MUTAGENESIS;

ARMORACIA RUSTICANA;

BIOSENSING TECHNIQUES; CYSTEINE; ELECTROCHEMISTRY; ELECTRON TRANSPORT; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; GOLD; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; HYDROGEN-ION CONCENTRATION; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 0036883691     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(02)00087-8     Document Type: Article

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