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3H]uridine (Amersham; 25 to 30 Ci/mmol) for 5 min at the same time points used in the DNA synthesis experiments. Ten percent TCA was added to stop the reaction, which was then held on ice for 30 min. Precipitates were collected on 0.45-μm Millipore GF/A filters and rinsed with 5% TCA. The filters were dried and counted. Growth-inhibited cultures [LB-novobiocin (50 μM)] were compared with rifampicin-treated cultures [LB-rifampicin (150 μg/mL)) and growing cultures (LB alone).
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0345297931
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+ (ES943) was obtained from E. Siegal.
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43
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0345729496
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note
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We thank G. Shadel and Y. W. Kow for critically reading this manuscript. This work was supported by NIH grants CA73041 and CA78622. A.V. was supported by the Medical Scientist Training Program at Emory University. H.J.Y. was supported by Chosun University (Republic of Korea) and the KOSEF postdoctoral fellowship program.
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