The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains

Yeast

Volumn 20, Issue 15, 2003, Pages 1263-1272

  Jeppsson, Marie ^a   Johansson, Björn ^a,c   Jensen, Peter Ruhdal ^b   Hahn Hägerdal, Bärbel ^a   Gorwa Grauslund, Marie F ^a  

^a LUND UNIVERSITY   (Sweden)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c UNIVERSITY OF MINHO   (Portugal)

Author keywords

Glucose 6 phosphate dehydrogenase; Inhibitors; NADPH; Redox balance; Saccharomyces cerevisiae; Synthetic promoter library; Xylitol; Xylose fermentation

Indexed keywords

5 HYDROXYMETHYLFURFURAL; ALCOHOL; COPPER; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLYCEROL; HYDROGENASE; HYDROXIDE; LIGNOCELLULOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PLASMID VECTOR; PROTEIN INHIBITOR; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; XYLITOL; XYLOSE;

ALCOHOL PRODUCTION; ARTICLE; AZOTOBACTER VINELANDII; CELLULAR DISTRIBUTION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATION ANALYSIS; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME SYNTHESIS; FERMENTATION; FERMENTATION MEDIUM; FUNGUS GROWTH; GENE LIBRARY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SENSITIVITY ANALYSIS; WILD TYPE;

ACETATES; BASE SEQUENCE; CARRIER PROTEINS; CELLULOSE; DNA, FUNGAL; ETHANOL; FERMENTATION; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSEPHOSPHATE DEHYDROGENASE; GLYCEROL; LIGNIN; METALLOTHIONEIN; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; XYLITOL; XYLOSE;

AZOTOBACTER; AZOTOBACTER VINELANDII; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0345329541     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1043     Document Type: Article

References (38)

1. Ausubel FM, Brent R, Kingston E, et al. 1995. Current Protocols in Molecular Biology; John Wiley & Sons, Inc.: New York.
2. Baker HV. 1986. Glycolytic gene expression in Saccharomyces cerevisiae: nucleotide sequence of GCR1, null mutants, and evidence for expression. Mol Cell Biol 6: 3774-3784.
3. Baker HV. 1991. GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif. Proc Natl Acad Sci USA 88: 9443-9447.
4. Bergmeyer H. 1974. Methods in Enzymatic Analysis. Academic Press: New York.
5. Bruinenberg P, de Bot P, van Dijken J, Scheffers A. 1983a. The role of redox balances in the anaerobic fermentation of xylose by yeasts. Eur J Appl Microbiol Biotechnol 18: 287-292.
6. Bruinenberg PM, van Dijken JP, Scheffers WA. 1983b. A theoretical analysis of NADPH production and consumption in yeasts. J Gen Microbiol 129: 953-964.
7. Drazinic CM, Smerage JB, Lopez MC, Baker HV. 1996. Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p). Mol Cell Biol 16: 3187-3196.
8. Eliasson A, Christensson C, Wahlbom CF, Hahn-Hägerdal B. 2000. Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures. Appl Environ Microbiol 66: 3381-3386.
9. Entian KD, Kötter P. 1998. Yeast mutant and plasmid collections. Yeast Gene Analysis, vol 26, Tuite AJP, Brown MF (eds). Academic Press: London; 431-449.
10. Gietz RD, Sugino A. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
11. Gietz D, St Jean A, Woods RA, Schiestl RH. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20: 1425.
12. Güldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
13. Hermann H, Hacker U, Bandlow W, Magdolen V. 1992. pYLZ vectors: Saccharomyces cerevisiae-Escherichia coli shuttle plasmids to analyze yeast promoters. Gene 119: 137-141.
14. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96: 23-28.
15. Jensen PR. 1997. Patent PCT/DK97/00342.
16. Jensen PR, Hammer K. 1998a. Artificial promoters for metabolic optimization. Biotechnol Bioeng 58: 191-195.
17. Jensen PR, Hammer K. 1998b. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl Environ Microbiol 64: 82-87.
18. Jeppsson M, Johansson B, Hahn-Hägerdal B, Gorwa-Grauslund M. 2002. Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose. Appl Environ Microbiol 68: 1604-1609.
19. Jeppsson M, Träff K, Johansson B, Hahn-Hägerdal B, Gorwa-Grauslund M. 2003. Effect of enhanced xylose reductase activity on xylose consumption and product distribution in xylose-fermenting recombinant Saccharomyces cerevisiae. FEMS Yeast Res 3: 167-175.
20. Kostrzynska M, Sopher CR, Lee H. 1998. Mutational analysis of the role of the conserved lysine-270 in the Pichia stipitis xylose reductase. FEMS Microbiol Lett 159: 107-112.
21. Labbe S, Thiele DJ. 1999. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol 306: 145-153.
22. Larsson S, Palmqvist E, Hahn-Hägerdal B, et al. 1999. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Technol 24: 151-159.
23. Miller JH. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press: New York.
24. Nieuwint RT, Mager WH, Maurer KC, Planta RJ. 1989. Mutational analysis of the upstream activation site of yeast ribosomal protein genes. Curr Genet 15: 247-251.
25. Nilvebrant N-O, Persson P, Reimann A, et al. 2003. Limits for alkaline detoxification of dilute acid lignocellulose hydrolysates. Appl Biochem Biotechnol 107: 615-628.
26. Nissen TL, Anderlund M, Nielsen J, Villadsen J, Kielland-Brandt MC. 2001. Expression of a cytoplasmic transhydrogenase in Saccharomyces cerevisiae results in formation of 2-oxoglutarate due to depletion of the NADPH pool. Yeast 18: 19-32.
27. Nogae I, Johnston M. 1990. Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase. Gene 96: 161-169.
28. Pampulha ME, Loureiro-Dias MC. 1989. Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast. Appl Microbiol Biotechnol 31: 547-550.
29. Pampulha ME, Loureiro-Dias MC. 2000. Energetics of the effect of acetic acid on growth of Saccharomyces cerevisiae. FEMS Microbiol Lett 184: 69-72.
30. Rotenberg MO, Woolford JL Jr. 1986. Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes. Mol Cell Biol 6: 674-687.
31. Tornoe J, Kusk P, Johansen TE, Jensen PR. 2002. Generation of a synthetic mammalian promoter library by modification of sequences spacing transcription factor binding sites. Gene 297: 21-32.
32. Uemura H, Koshio M, Inoue Y, Lopez MC, Baker HV. 1997. The role of Gcr1p in the transcriptional activation of glycolytic genes in yeast Saccharomyces cerevisiae. Genetics 147: 521-532.
33. Wach A, Brachat A, Pohlmann R, Philippsen P. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
34. Wahlbom CF, Hahn-Hägerdal B. 2002. Furfural, 5-hydroxymethyl furfural, and acetoin act as external electron acceptors during anaerobic fermentation of xylose in recombinant Saccharomyces cerevisiae. Biotechnol Bioeng 78: 172-178.
35. Verduyn C, Postma E, Scheffers WA, Van Dijken JP. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501-517.
36. Verho R, Richard P, Jonson PH, et al. 2002. Identification of the first fungal NADP-GAPDH from Kluyveromyces lactis. Biochemistry 41: 13 833-13 838.
37. Voordouw G, Veeger C, Van Breemen JF, Van Bruggen EF. 1979. Structure of pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Eur J Biochem 98: 447-454.
38. Zhang Y, Lee H. 1997. Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase. FEMS Microbiol Lett 147: 227-232.