Alkylation of myotoxic phospholipases A2 in Bothrops moojeni venom: A promising approach to an enhanced antivenom production

International Journal of Biochemistry and Cell Biology

Volumn 36, Issue 2, 2004, Pages 258-270

  Soares, Andreimar M ^a   Sestito, Wladimir P ^b,c   Marcussi, Silvana ^a   Stábeli, Rodrigo G ^b   Andrião Escarso, Silvia H ^b   Cunha, Odete A B ^b   Vieira, Carlos A ^b   Giglio, José R ^b  

^a UNIVERSITY OF RIBEIRÃO PRETO   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c FACULDADE DE MEDICINA DE SÃO JOSÉ DO RIO PRETO   (Brazil)

Author keywords

Alkylation; Antigenicity; Antivenom; Bothrops moojeni; Myotoxins; Phospholipase A2; Snake venom

Indexed keywords

4 BROMOPHENACYL BROMIDE; PHOSPHOLIPASE A2; SNAKE VENOM; VENOM ANTISERUM;

ALKYLATION; AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANTIGENICITY; ARTICLE; CROSS REACTION; DRUG MANUFACTURE; EDEMA; LETHAL DOSE; MALE; MOUSE; MUSCLE NECROSIS; NONHUMAN; PROTEIN STRUCTURE; SNAKE;

BOTHROPS; BOTHROPS MOOJENI; ORYCTOLAGUS CUNICULUS; SERPENTES;

EID: 0344413739     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(03)00237-1     Document Type: Article

References (42)

1. 2 isolated from the venom of the snake Bothrops asper (Viperidae). Revista de Biologia Tropical. 36:1988;563-565.
2. 2 from Bothrops jararacussu snake venom. Biochemical Pharmacology. 64:2002;723-732.
3. 2 in Bothrops snake venoms: Effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu. Biochimie. 82:2000;755-763.
4. Angulo Y., Estrada R., Gutiérrez J.M. Clinical and laboratory alterations in horses during immunization with snake venoms for the production of polyvalent (Crotalinae) antivenom. Toxicon. 35:1997;81-90.
5. 2 from Bothrops asper snake venom. Toxicon. 39:2001;879-887.
6. Borkow G., Gutiérrez J.M., Ovadia M. Inhibition of toxic activities of Bothrops asper venom and other crotalid snake venoms by a novel neutralizing mixture. Toxicology and Applied Pharmacology. 147:1997;442-447.
7. Campbell, D. H., Garvey, J. S., Cremer, N. E., & Sussdorf, D. H. (1970). In W. A. Denjamin (Ed.), Methods in immunology (vol. 260). New York: W.A. Benjamin Inc.
8. Chaves F., Barboza M., Gutiérrez J.M. Pharmacological study of edema induced by venom of the snake Bothrops asper (Terciopelo) in mice. Toxicon. 33:1995;31-39.
9. de Haas G.H., Postema N.M., Nieuwenhuizen W., van Deenen L.L. Purification and properties of phospholipase A from porcine pancreas. Biochimica et Biophysica Acta. 159:1968;103-117.
10. de Roodt A.R., Dolar J.A., Fernandez T., Segre L., Hajos S.E. Cross-reactivy and heterologous neutralization of crotaline antivenoms used in Argentina. Toxicon. 36:1998;1025-1038.
11. 2 isolated from Bothrops asper and Bothrops godmani snake venoms: Effects on enzymatic and pharmacological properties. Toxicon. 35:1997;241-252.
12. Domont G.B., Perales J., Moussatche H. Natural anti-snake venom proteins. Toxicon. 29:1991;1183-1194.
13. 2 myotoxins lyse cell cultures by two distincts mechanisms. Toxicon. 36:1998;1549-1555.
14. 2 myotoxins from Bothrops snake venoms. Review article. Toxicon. 33:1995;1405-1424.
15. Gutiérrez J.M., Avila C., Rojas E., Cerdas L. An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica. Toxicon. 26:1988;411-413.
16. Itzhaki R.F., Gill D.M. A micro-biuret method for estimating proteins. Analytical Biochemistry. 9:1964;401-410.
17. Kouyoumdjian J.A., Polizeli C., Lobo S.M.A., Guimarães S.M. Acidentes ofídicos causados por Bothrops moojeni na região de São José do Rio Preto - SP. Arquivos Brasileiros de Medicina. 64:1990;167-171.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
19. 2EDTA of local tissue damage induced by Bothrops asper (Terciopelo) venom: Application in horse immunization for antivenom production. Toxicon. 36:1998;321-331.
20. 2 purified from porcine pancreas and snake venoms. Toxicon. 31:1993;949-956.
21. Lomonte B., Gutiérrez J.M., Furtado M.F., Otero R., Rosso J.P., Vargas O., Carmona E., Rovira M.E. Isolation of basic myotoxins from Bothrops moojeni and Bothrops atrox snake venoms. Toxicon. 28:1990;1137-1146.
22. Ministério da Saúde do Brasil, 1998. Manual de Diagnóstico e Tratamento de Acidentes por Animais Peçonhentos (pp. 7-25). Brasília.
23. Nikai T., Mori N., Kishida M., Sugihara H., Tu A.T. Isolation and biochemical charcterization of hemorrhagic toxin from the venom of Crotalus atrox. Archives of Biochemistry and Biophysics. 231:1984;309-319.
24. Ownby C.L. Structure, function and biophysical aspects of the myotoxins from snake venoms. Journal of Toxicology - Toxins Review. 17:1998;213-238.
25. 2 proteins. Toxicon. 37:1999;411-445.
26. Reisfeld R.A., Lewis J., Willians D.A. Disk electrophoresis of basic proteins and peptides on polyacrylamide gel. Nature. 4838:1962;281.
27. Rodrigues V.M., Soares A.M., Mancin A.C., Fontes M.R.M., Homsi-Brandeburgo M.I., Giglio J.R. Geographic variations in the composition of myotoxins from Bothrops neuwiedi snake venoms: Biochemical characterization and biological activity. Comparative Biochemistry and Physiology. 121:1998;215-222.
28. Rosenberg, P. (1986). The relationship between enzymatic activity and pharmacological properties of phospholipses in natural poisom. In J. B. Harris (Ed.), Natural toxins: Animal, plant and microbial. Oxford: Clarendon Press.
29. Rosenfeld, G. (1971). Symptomatology, pathology and treatment of snake bites in South America. In W. Bucler, & E. Buckley (Eds.), Venomous animals and their venoms (pp. 345-384). New York: Academic Press.
30. Rucavado A., Gutiérrez J.M. Snake venom metalloproteinases: Their role in the pathogenesis of local tissue damage. Review article. Biochimie. 82:2000;841-850.
31. Sampaio S.V., Laure C.J., Giglio J.R. Isolation and characterization of toxic proteins from the venom of the Brazilian scorpion Tytius serrulatus. Toxicon. 21:1983;265-277.
32. Selvanayagam Z.E., Gopalakrishnakone P. Tests for detection of snake venoms. Toxicon. 37:1999;565-586.
33. 2 homologue from Bothrops moojeni (Caissaca) snake venom. Archives of Biochemistry and Biophysics. 373:2000;7-15.
34. 2 from Bothrops pirajai snake venom. Archives of Biochemistry and Biophysics. 387:2001;188-196.
35. 2 homologue from Bothrops neuwiedi pauloensis venom. Archives of Biochemistry and Biophysics. 378:2000;201-209.
36. 2 subunit of crotoxin from Crotalus durissus terrificus snake venom, on its enzymatic and pharmacological activities. International Journal of Biochemistry and Cell Biology. 33:2001;877-888.
37. Soares A.M., Rodrigues V.M., Homsi-Brandeburgo M.I., Toyama M.H., Lombardi F.R., Arni R.K., Giglio J.R. A rapid procedure for the isolation of the Lys-49 myotoxin II from Bothrops moojeni (Caissaca) venom: Biochemical characterization, crystallization, myotoxic and edematogenic activity. Toxicon. 36:1998;503-514.
38. Trento E.P., Garcia O.S., Rucavado A., França S.C., Batalini C., Arantes E.C., Giglio J.R., Soares A.M. Inhibitory properties of anti-bothropic complex from Didelphis albiventris serum on toxic and pharmacological actions of metaloproteases and myotoxins from Bothrops asper venom. Biochemical Pharmacology. 62:2001;1521-1529.
39. Vital Brazil, O. (1982). Peçonhas. In C. E. Corbett (Ed.), Farmacodinâmica. Rio de Janeiro, Brazil: Editora Guanabara-Koogan.
40. 50) and instruction in their use. Pittsburgh, PC: Mellan Institute.
41. World Health Organization (1981). Progress in the characterization of venoms and standardization of antivenoms. Geneva.
42. 2 modified by p-bromo-phenacyl-bromide. Toxicon. 36:1998;875-886.