An interference-free fluorescent assay of telomerase for the high-throughput analysis of inhibitors

Analytical Biochemistry

Volumn 323, Issue 1, 2003, Pages 65-73

  Francis, Rawle ^a   Friedman, Simon H ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; PROTEINS;

FALSE POSITIVE; FLUORESCENT ASSAYS; HIGH-THROUGHPUT ANALYSIS; INTERFERENCE-FREE; LIQUID/LIQUID EXTRACTION; PREAMPLIFICATION; TAQ POLYMERASE; TELOMERASE ACTIVITY;

POLYMERASE CHAIN REACTION;

ACRIDINE ORANGE; DOXORUBICIN; ETHACRIDINE LACTATE; ETHIDIUM BROMIDE; INTERCALATING AGENT; TELOMERASE; TELOMERASE INHIBITOR;

ANALYTIC METHOD; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ASSAY; HELA CELL; HUMAN; HUMAN CELL; IC 50; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0242690429     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.08.021     Document Type: Article

References (12)

1. Kim N.W., Piatyszek M.A., Prowse K.R., Harley C.B., West M.D., Ho P.L.C., Coviello G.M., Wright W.E., Weinrich S.L., Shay J.W. Specific association of human telomerase activity with immortal cells and cancer. Science. 266:1994;2011-2015.
2. Autexier C. Telomerase as a possible target for anticancer therapy. Chem. Biol. 6:1999;R299-R303.
3. Corey D.R. Telomerase: an unusual target for cytotoxic agents. Chem. Res. Toxicol. 13:2000;957-960.
4. Morin G.B. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell. 59:1989;521-529.
5. Gelmini S., Caldini A., Becherini L., Capaccioli S., Pazzagli M., Orlando C. Rapid, quantitative nonisotopic assay for telomerase activity in human tumors. Clin. Chem. 44:1998;2133-2138.
6. West C., Francis R., Friedman S.H. Small molecule/nucleic acid affinity chromatography: application for the identification of telomerase inhibitors which target its key RNA/DNA heteroduplex. Bioorg. Med. Chem. Lett. 11:2001;2727-2730.
7. Francis R., West C., Friedman S.H. Targeting telomerase via its key RNA-DNA heteroduplex. Bioorg. Chem. 29:2001;107-117.
8. Ueno T., Takahashi H., Oda M., Mizunuma M., Yokoyama A., Goto Y., Mizushina Y., Sakaguchi K., Hayashi H. Inhibition of human telomerase by rubromycins: implication of spiroketal system of the compounds as an active moiety. Biochemistry. 39:2000;5995-6002.
9. Wright W.E., Shay J.W., Piatyszek M.A. Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity. Nucleic Acids. Res. 23:1995;3794-3795.
10. Francis R., Friedman S.H. A rapid direct telomerase assay method using 96-well streptavidin plates. BioTechniques. 32:2002;1154-1160.
11. Sun D., Hurley L.H., Von Hoff D.D. Telomerase assay using biotinylated-primer extension and magnetic separation of the products. BioTechniques. 25:1998;1046-1051.
12. Gan Y., Ju J., Johnson A., Wientjes M.G., Schuller D.E., Au J.L.S. A quantitative assay of telomerase activity. Pharm. Res. 18:2001;488-493.