Rapid, quantitative nonisotopic assay for telomerase activity in human tumors

Clinical Chemistry

Volumn 44, Issue 10, 1998, Pages 2133-2138

  Gelmini, Stefania ^a   Caldini, Anna ^b   Becherini, Lucia ^a   Capaccioli, Sergio ^a   Pazzagli, Mario ^a   Orlando, Claudio ^a,c  

^a UNIVERSITY OF FLORENCE   (Italy)

^b CAREGGI UNIVERSITY HOSPITAL   (Italy)

^c Dept of Clinical Physiopathology ^*  (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

TELOMERASE;

ARTICLE; BREAST CANCER; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ENDOMETRIUM CANCER; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HUMAN TISSUE; KIDNEY CANCER; MENINGIOMA; OVARY CANCER; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; QUANTITATIVE DIAGNOSIS; STOMACH CANCER; TUMOR DIAGNOSIS; TUMOR GROWTH;

EID: 0031719991     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.10.2133     Document Type: Article

References (31)

1. Rhyu MS. Telomeres, telomerase, and immortality. J Natl Cancer Inst 1995;87:884-94.
2. Zakian VA. Structure and function of telomeres. Annu Rev Genet 1989;23:579-604.
3. Blackburn EH. Structure and function of telomerase. Nature 1991;350:569-73.
4. n+ present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A 1988;85:6622-6.
5. Morin GB. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 1989; 59:521-9.
6. Blackburn EH. Telomeres: no end in sight. Cell 1994;77:621-3.
7. Dahse R, Fiedler W, Ernst G. Telomeres and telomerase: biological and clinical importance. Clin Chem 1997;43:708-14.
8. Harley CB, Futcher AB, Greider CW. Telomeres shorten during aging of human fibroblasts. Nature 1990;345:458-60.
9. Levy MZ, Allsopp RC, Futcher AB, Greider CW, Harley CB. Telomere end-replication problem and cell aging. J Mol Biol 1992;225:951-60.
10. Lingner J, Cooper JP, Cech TR. Telomerase and DNA end replication: no longer a lagging strand problem? Science 1995;269: 1533-4.
11. Morin GB. Is telomerase a universal cancer target? J Natl Cancer Inst 1995;87:859-60.
12. Shay JW, Bacchetti S. A survey of telomerase activity in human cancer. Eur J Cancer 1997;33:787-91.
13. Burger AM, Bibby MC, Double JA. Telomerase activity in normal and malignant tissues: feasibility of telomerase as a target for cancer chemotherapy. Br J Cancer 1997;75:516-22.
14. Kim NW. Clinical implications of telomerase in cancer. Eur J Cancer 1997;33:781-6.
15. Breslow RA, Shay JW, Gadzar AF, Srivastava S. Telomerase and early detection of cancer: a National Cancer Institute workshop. J Natl Cancer Inst 1997;89:618-23.
16. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PCL, et al. Specific association of human telomerase activity with immortal cells and cancer. Science 1994;266:2011-5.
17. Tatematsu K, Nakayama J, Danbara M, Shinoia S, Sato H, Omine M, Ishikawa. A novel quantitative 'stretch PCR assay', that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias. Oncogene 1996;13:2265-74.
18. Wright WE, Shay JW, Piatyszek MA. Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity. Nucleic Acids Res 1995;23:3794-5.
19. Ohyashiki JH, Ohjashiki K, Sano T, Toyama K. Non-radioisotopic and semi-quantitative procedure for terminal repeat amplification protocol. Jpn J cancer Res 1996;87:329-31.
20. Savoisky E, Akamatsu K, Tsuchiya M, Yamazaki T. Detection of telomerase activity by combination of TRAP method and scintillation proximity assay (SPA). Nucleic Acids Res 1996;24:1175-6.
21. Aldous WK, Grabil NR. A fluorescent method for detection of telomerase activity. Diagn Mol Pathol 1997;6:102-10.
22. Hirose M, Hashimoto JA, Ogura K, Tahara H, Ide T, Yoshimura T. A rapid, useful and quantitative method to measure telomerase activity by hybridization protection assay connected with a telomeric repeat amplification protocol. J Cancer Res Clin Oncol 1997;123:337-44.
23. Ahn SJ, Costa J, Emanuel JR. PicoGreen quantitation of DNA: effective evaluation of samples pre- and post-PCR. Nucleic Acids Res 1996;24:2623-5.
24. Hiyama K, Hiyama E, Ishioka S, Yamakido M, Inai K, Gazdar AF, et al. Telomerase activity in small-cell and non-small-cell lung cancer. J Natl Cancer Inst 1995;87:895-902.
25. Hiyama E, Hiyama K, Yokoyama T, Matsuura Y, Piatyszek MA, Shay JW. Correlating telomerase activity levels with human neuroblastoma outcomes. Nat Med 1995;1:249-55.
26. Kobelmann-Betzing C, Seeger K, Dragon S, Schmitt G, Moricke A, Schild TA, et al. Advantages of new Taq DNA polymerase in multiplex PCR and time-release PCR. Biotechniques 1998;24: 154-8.
27. Shay JW, Wright WE. Telomerase activity in human cancer. Curr Opin Oncol 1996;8:66-71.
28. Nakamura TM, Morin GB, Chapman KB, Weinrich SL, Andrews WH, Lingner I, et al. Telomerase catalytic subunit homologs from fission yeast and human. Science 1997;277:955-9.
29. Meyerson M, Counter CM, Ng Eaton E, Ellisen LW, Steiner P, Dickinson Caddle S, et al. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 1997;90:785-95.
30. Zhu X, Kumar R, Mandal M, Sharma N, Sharma HW, Dhingra U, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc Natl Acad Sci U S A 1996;93:6091-5.
31. Langford LA, Piatyszek MA, Xu R, Schold SC, Wright WE, Shay JW. Telomerase activity: a prognostic indicator in ordinary meningioma. Hum Pathol 1997;28:416-20.