Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore

Biochemical Journal

Volumn 375, Issue 3, 2003, Pages 633-641

  Ramjeesingh, Mohabir ^a   Ugwu, Francisca ^a   Li, Canhui ^a   Dhani, Sonja ^a   Huan, Ling Jun ^a   Wang, Yanchun ^a   Bear, Christine E ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Chloride selective channel; Cysteine modification; Cystic fibrosis transmembrane conductance regulator (CFTR); Dimer; Glycosylation; Second membrane spanning domain

Indexed keywords

BIOLOGICAL MEMBRANES; CHLORIDE MINERALS; DIMERS; FLUXES; NEGATIVE IONS; PROTEINS;

LIPOSOMES; PLASMA MEMBRANES;

BIOCHEMISTRY;

CHLORIDE CHANNEL; LIPOSOME; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; CELL MEMBRANE; CHLORIDE TRANSPORT; CONTROLLED STUDY; DISSOCIATION; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN QUATERNARY STRUCTURE; PURIFICATION;

ANIMALS; ANIONS; BINDING SITES; CELL LINE; CELL MEMBRANE; CHLORIDE CHANNELS; CHROMATOGRAPHY, ION EXCHANGE; CIRCULAR DICHROISM; CYSTEINE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DIFFUSION; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPHYSIOLOGY; LIPOSOMES; MEMBRANE PROTEINS; PHOSPHOLIPIDS; SPODOPTERA;

UNIDENTIFIED BACULOVIRUS;

EID: 0242637061     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030774     Document Type: Article

References (40)

1. Welsh, M. J. and Smith, A. E. (1995) Cystic fibrosis. Sci. Am. 273, 52-59
2. Tsui, L. C. and Durie, P. (1997) Genotype and phenotype in cystic fibrosis. Hosp. Pract. (Off. Ed.) 32, 115-118, 123-129, 134
3. Riordan, J. R., Rommens, J. M., Kerem, B., Alon, M., Rozmahel, R., Grzelczak, Z., Zielenski, J., Lok, S., Plavsic, N., Chou, J. L. et al. (1989) Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245, 1066-1073
4. Chang, G. and Roth, C. B. (2001) Structure of MsbA from E. coli: a homolog of the multidrug resistance ATP binding cassette (ABC) transporters. Science 293, 1793-1800
5. Locher, K. P., Lee, A. T. and Rees, D. C. (2002) The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism. Science 296, 1091-1098
6. Linsdell, P., Evagelidis, A. and Hanrahan, J. W. (2000) Molecular determinants of anion selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel pore. Biophys. J. 78, 2973-2982
7. Sheppard, D. N. and Welsh, M. J. (1999) Structure and function of the CFTR chloride channel. Physiol. Rev. 79 (Suppl. 1), S23-S45
8. - channels expressed in a mammalian cell line and its regulation by a critical pore residue. J. Physiol. (Cambridge, U.K.) 496, 687-693
9. - channel pore. J. Physiol. (Cambridge, U.K.) 540, 39-47
10. Tabcharani, J. A., Linsdell, P. and Hanrahan, J. W. (1997) Halide permeation in wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels. J. Gen. Physiol. 110, 341-354
11. Linsdell, P., Tabcharani, J. A., Rommens, J. M., Hou, Y. X., Chang, X. B., Tsui, L. C., Riordan, J. R. and Hanrahan, J. W. (1997) Permeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anions. J. Gen. Physiol. 110, 355-364
12. Linsdell, P. Tabcharani, J. A. and Hanrahan, J. W. (1997) Multi-ion mechanism for ion permeation and block in the cystic fibrosis transmembrane conductance regulator chloride channel. J. Gen. Physiol. 110, 365-377
13. Smith, S. S., Liu, X, Zhang, Z. R., Sun, F., Kriewall, T. E., McCarty, N. A. and Dawson, D. C. (2001) CFTR: covalent and noncovalent modification suggests a role for fixed charges in anion conduction. J. Gen. Physiol. 118, 407-431
14. 352 is a major determinant of charge selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel. Biochemistry 38, 5528-5537
15. Cheung, M. and Akabas, M. H. (1997) Locating the anion-selectivity filter of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. J. Gen. Physiol. 109, 289-299
16. Tabcharani, J. A., Rommens, J. M., Hou, Y. X., Chang, X. B., Tsui, L. C., Riordan, J. R. and Hanrahan, J. W. (1993) Multi-ion pore behaviour in the CFTR chloride channel. Nature (London) 366, 79-82
17. Gong, X. and Linsdell, P. (2003) Molecular determinants and role of an anion binding site in the external mouth of the CFTR chloride channel pore. J. Physiol. (Cambridge, U.K.) 549, 387-391
18. Donough, S., Davidson, M., Lester, H. A. and McCarty, N. A. (1994) Novel pore-lining residues in CFTR that govern permeation and open-channel block. Neuron 13, 623-634
19. - channel expressed in mammalian cell lines. J. Physiol. (Cambridge, U.K.) 512, 1-16
20. - channel by butyrate and phenylbutyrate. Eur. J. Pharmacol. 411, 255-260
21. - channel. Cell (Cambridge, Mass.) 76, 1091-1098
22. Devidas, S., Yue, H. and Guggino, W. B. (1998) The second half of the cystic fibrosis transmembrane conductance regulator forms a functional chloride channel. J. Biol. Chem. 273, 29373-29380
23. Gupta, J., Evagelidis, A., Hanrahan, J. W. and Linsdell, P. (2001) Asymmetric structure of the cystic fibrosis transmembrane conductance regulator chloride channel pore suggested by mutagenesis of the twelfth transmembrane region. Biochemistry 40, 6620-6627
24. Zhang, Z. R., McDonough, S. I. and McCarty, N. A. (2000) Interaction between permeation and gating in a putative pore domain mutant in the cystic fibrosis transmembrane conductance regulator. Biophys. J. 79, 298-313
25. McCarty, N. A. (2000) Permeation through the CFTR chloride channel. J. Exp. Biol. 203, 1947-1962
26. Ramjeesingh, M., Li, C., Kogan, I., Wang, Y., Huan, L. J. and Bear, C. E. (2001) A monomer is the minimum functional unit required for channel and ATPase activity of the cystic fibrosis transmembrane conductance regulator. Biochemistry 40, 10700-10706
27. Kogan, I., Ramjeesingh, M., Li, C., Kidd, J. F., Wang, Y., Leslie, E. M., Cole, S. P. and Bear, C. E. (2003) CFTR directly mediates nucleotide-regulated glutathione flux. EMBO J. 22, 1981-1989
28. 27a Rabilloud, T., Vuillard, L., Gilly, C. and Lawrence, J. J. (1994) Silver-staining of proteins in polyacrylamide gels: general overview. Cell. Mol. Biol. 40, 57-75
29. 27b Peterson, G. L. (1977) Simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346-356
30. Goldberg, A. F. and Miller, C. (1991) Solubilization and functional reconstitution of a chloride channel from Torpedo California electroplax. J. Membr. Biol. 124, 199-206
31. Long, J. C., Wang, S. and Vik, S. B. (1998) Membrane topology of subunit a of the F1F0 ATP synthase as determined by labeling of unique cysteine residues. J. Biol. Chem. 273, 16235-16240
32. Bayer, E. A., Ben-Hur, H. and Wilchek, M. (1988) Biocytin hydrazide - a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal. Biochem. 170, 271-281
33. O'Riordan, C. R., Lachapelle, A. L., Marshall, J., Higgins, E. A. and Cheng, S. H. (2000) Characterization of the oligosaccharide structures associated with the cystic fibrosis transmembrane conductance regulator. Glycobiology 10, 1225-1233
34. Hammerle, M. M., Aleksandrov, A. A., Chang, X. B. and Riordan, J. R. (2000) A novel CFTR disease-associated mutation causes addition of an extra N-linked oligosaccharide. Glycoconj. J. 17, 807-813
35. Maduke, M., Pheasant, D. J. and Miller, C. (1999) High-level expression, functional reconstitution, and quaternary structure of a prokaryotic CIC-type chloride channel. J. Gen. Physiol. 114, 713-722
36. Tabcharani, J. A., Chang, X. B., Riordan, J. R. and Hanrahan, J. W. (1992) The cystic fibrosis transmembrane conductance regulator chloride channel. Iodide block and permeation. Biophys. J. 62, 1-4
37. Gregory, E. M. (1975) Chemical modification of bovine heart mitochondrial malate dehydrogenase. Selective modification of cysteine and histidine. J. Biol. Chem. 250, 5470-5474
38. Gotten, J. F. and Welsh, M. J. (1997) Covalent modification of the regulatory domain irreversibly stimulates cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 272, 25617-25622
39. + channel. Nature (London) 423, 33-41
40. 2-terminal nucleotide binding domain. J. Gen. Physiol. 116, 163-180