Molecular determinants and role of an anion binding site in the external mouth of the CFTR chloride channel pore

Journal of Physiology

Volumn 549, Issue 2, 2003, Pages 387-397

  Gong, Xiandi ^a   Linsdell, Paul ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; CHLORIDE; CHLORIDE CHANNEL; GOLD DERIVATIVE; TRANSMEMBRANE CONDUCTANCE REGULATOR; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CHLORIDE TRANSPORT; CONTROLLED STUDY; ELECTRICITY; GENE MUTATION; ION TRANSPORT; MOLECULAR INTERACTION; NONHUMAN; PH; PRIORITY JOURNAL; SURFACE CHARGE; AMINO ACID SUBSTITUTION; ANION TRANSPORT; CHANNEL GATING; CHLORIDE CONDUCTANCE; CHLORIDE CURRENT; ELECTRIC POTENTIAL; EXTRACELLULAR MATRIX; INTRACELLULAR TRANSPORT; MUTAGENESIS; MUTATIONAL ANALYSIS; PROTEIN PROTEIN INTERACTION; REVERSAL POTENTIAL; STATIC ELECTRICITY;

EID: 0038120974     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.2002.038232     Document Type: Article

References (33)

1. + channel. Nature 414, 73-77.
2. Cheung M & Akabas MH (1996). Identification of cystic fibrosis transmembrane conductance regulator channel-lining residues in and flanking the M6 membrane-spanning segment. Biophys J 70, 2688-2695.
3. 2+ entering from the intracellular pore entrance. J Gen Physiol 116, 349-362.
4. Cotten JF & Welsh MJ (1999). Cystic fibrosis-associated mutations at arginine 347 alter the pore architecture of CFTR. Evidence for disruption of a salt bridge. J Biol Chem 274, 5429-5435.
5. Dawson DC, Smith SS & Mansoura MK (1999). CFTR: mechanism of anion conduction. Physiol Rev 79, S47-75.
6. + conduction and selectivity. Science 280, 69-77.
7. Dutzler R, Campbell EB, Cadene M, Chait BT & MacKinnon R (2002). X-ray structure of a ClC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity. Nature 415, 287-294.
8. 2+ interactions. Neuron 15, 1121-1132.
9. Fahlke C (2001). Ion permeation and selectivity in ClC-type chloride channels. Am J Physiol Renal Physiol 280, F748-757.
10. - channel pore. J Physiol 540, 39-47.
11. Gong X, Burbridge SM, Lewis AC, Wong PYD & Linsdell P (2002b). Mechanism of lonidamine inhibition of the CFTR chloride channel. Br J Pharmacol 137, 928-936.
12. Gong X & Linsdell P (2003). Coupled movement of permeant and blocking ions in the CFTR chloride channel pore. J Physiol 549, 375-385.
13. Gupta J, Evagelidis A, Hanrahan JW & Linsdell P (2001). Asymmetric structure of the cystic fibrosis transmembrane conductance regulator chloride channel pore suggested by mutagenesis of the twelfth transmembrane region. Biochemistry 40, 6620-6627.
14. Gupta J & Linsdell P (2002). Point mutations in the pore region directly or indirectly affect glibenclamide block of the CFTR chloride channel. Pflugers Arch 443, 739-747.
15. Jentsch TJ, Stein V, Weinreich F & Zdebik AA (2002). Molecular structure and physiological function of chloride channels. Physiol Rev 82, 503-568.
16. Linsdell P (2001). Relationship between anion binding and anion permeability revealed by mutagenesis within the cystic fibrosis transmembrane conductance regulator chloride channel pore. J Physiol 531, 51-66.
17. Linsdell P, Evagelidis A & Hanrahan JW (2000). Molecular determinants of anion selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel pore. Biophys J 78, 2973-2982.
18. - channel currents. J Physiol 540, 29-38.
19. - channel expressed in mammalian cell lines. J Physiol 512, 1-16.
20. McCarty NA (2000). Permeation through the CFTR chloride channel. J Exp Biol 203, 1947-1962.
21. McCarty NA & Zhang Z-R (2001). Identification of a region of strong discrimination in the pore of CFTR. Am J Physiol Lung Cell Mol Physiol 281, L852-867.
22. McCleskey EW (1999). Calcium channel permeation: a field in flux. J Gen Physiol 113, 765-772.
23. McDonough S, Davidson N, Lester HA & McCarty NA (1994). Novel pore-lining residues in CFTR that govern permeation and open-channel block. Neuron 13, 623-634.
24. Miller C (2000). Ion channels: doing hard chemistry with hard ions. Curr Opin Chem Biol 4, 148-151.
25. + selectivity filter. Nature 414, 37-42.
26. Radzicka A & Wolfenden R (1988). Comparing the polarities of the amino acids: side-chain distribution coefficients between the vapor phase, cyclohexane, 1-octanol, and neutral aqueous solution. Biochemistry 27, 1664-1670.
27. Riordan JF (1979). Arginyl residues and anion binding sites in proteins. Mol Cell Biochem 26, 71-92.
28. Smith SS, Liu X, Zhang Z-R, Sun F, Kriewall TE, McCarty NA & Dawson DC (2001). CFTR: covalent and noncovalent modification suggests a role for fixed charges in anion conduction. J Gen Physiol 118, 407-431.
29. Smith SS, Steinle ED, Meyerhoff ME & Dawson DC (1999). Cystic fibrosis transmembrane conductance regulator. Physical basis for lyotropic anion selectivity patterns. J Gen Physiol 114, 799-818.
30. Tabcharani JA, Linsdell P & Hanrahan JW (1997). Halide permeation in wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels. J Gen Physiol 110, 341-354.
31. Woodhull AM (1973). Ionic blockage of sodium channels in nerve. J Gen Physiol 61, 687-708.
32. 2+ channels. Nature 366, 158-161.
33. + channel-Fab complex at 2.0 Å resolution. Nature 414, 43-48.