Disulphonic stilbene block of cystic fibrosis transmembrane conductance regulator Cl- channels expressed in a mammalian cell line and its regulation by a critical pore residue

Journal of Physiology

Volumn 496, Issue 3, 1996, Pages 687-693

  Linsdell, Paul ^a   Hanrahan, John W ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; 4,4' DINITRO 2,2' STILBENEDISULFONIC ACID; ARGININE; ASPARTIC ACID; CHLORIDE CHANNEL; STILBENEDISULFONIC ACID; TRANSMEMBRANE CONDUCTANCE REGULATOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL STRAIN BHK; CHEMICAL STRUCTURE; CONTROLLED STUDY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL;

EID: 0029861859     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.1996.sp021719     Document Type: Article

References (21)

1. - channels in planar lipid bilayers. American Journal of Physiology 256, C902-912.
2. CHEUNG, M. & AKABAS, M. H. (1996). Identification of cystic fibrosis transmembrane conductance regulator channel-lining residues in and flanking the M6 membrane-spanning segment. Biophysical Journal 70, 2688-2695.
3. GADSBY, D. C., NAGEL, G. & HWANG, T.-C. (1995). The CFTR chloride channel of mammalian heart. Annual Review of Physiology 57, 387-416.
4. GRAY, M. A., POLLARD, C. E., HARRIS, A., COLEMAN, L., GREENWELL, J. R. & ARGENT, B. E. (1990). Anion selectivity and block of the small-conductance chloride channel on pancreatic duct cells. American Journal of Physiology 259, C752-761.
5. HANRAHAN, J. W., TABCHARANI, J. A. & GRYGORCZYK, R. (1993). Patch clamp studies of apical membrane chloride channels. In Cystic Fibrosis - Current Topics, ed. DODGE, J. A., BROCK, D. J. H. & WIDDICOMBE, J. H., pp. 93-137. Wiley, Chichester.
6. KARTNER, N., HANRAHAN, J. W., JENSEN, T. J., NAISMITH, A. L., SUN, S., ACKERLEY, C. A., REYES, E. F., TSUI, L.-C., ROMMENS, J. M., BEAR, C. E. & RIORDAN, J. R. (1991). Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance. Cell 64, 681-691.
7. LINSDELL, P. & HANRAHAN, J. W. (1996). Flickery block of single CFTR chloride channels by intracellular anions and osmolytes. American Journal of Physiology 271, C628-634.
8. LINSDELL, P., ROMMENS, J. M., HOU, Y.-X., CHANG, X.-B., TSUI, L.-C., RIORDAN, J. R. & HANRAHAN, J. W. (1996). Mutation of the narrow pore region of the CFTR chloride channel pore. Biophysical Journal 70, A72.
9. - channel by two closely related arylaminobenzoates. Journal of General Physiology 102, 1-23.
10. MCDONOUGH, S., DAVIDSON, N., LESTER, H. A. & MCCARTY, N. A. (1994). Novel pore-lining residues in CFTR that govern permeation and open-channel block. Neuron 13, 623-634.
11. PASSOW, H. (1987). Molecular aspects of band-3 protein-mediated anion transport across the red blood cell membrane. Reviews of Physiology and Biochemistry 103, 61-203.
12. PASSOW, H., WOOD, P. G., LEPKE, S., MÜLLER, H. & SOVAK, M. (1992). Exploration of the functional significance of the stilbene disulfonate binding site in mouse band 3 by site-directed mutagenesis. Biophysical Journal 62, 98-100.
13. SCHWIEBERT, E. M., FLOTTE, T., CUTTING, G. R. & GUGGINO, W. B. (1994). Both CFTR and outwardly rectifying chloride channels contribute to cAMP-stimulated whole cell chloride currents. American Journal of Physiology 266, C1464-1477.
14. SEIBERT, F. S., TABCHARANI, J. A., CHANG, X.-B., DULHANTY, A. M., MATHEWS, C., HANRAHAN, J. W. & RIORDAN, J. R. (1995). cAMP-dependent protein kinase-mediated phosphorylation of cystic fibrosis transmembrane conductance regulator residue Ser-753 and its role in channel activation. Journal of Biological Chemistry 270, 2158-2162.
15. SHEN, B. Q., MRSNY, R. J., FINKBEINER, W. E. & WIDDICOMBE, J. H. (1995). Role of CFTR in chloride secretion across human tracheal epithelium. American Journal of Physiology 269, L561-566.
16. SINGH, A. K., AFINK, G. B., VENGLARIK, C. J., WANG, R. & BRIDGES, R. J. (1991). Colonic Cl channel blockade by three classes of compounds. American Journal of Physiology 260, C51-63.
17. - channel in CHO cells stably expressing the cystic fibrosis gene. Nature 352, 628-631.
18. 84. FEBS Letters 270, 157-164.
19. TABCHARANI, J. A., ROMMENS, J. M., HOU, Y.-X., CHANG, X.-B., TSUI, L.-C., RIORDAN, J. R. & HANRAHAN, J. W. (1993). Multi-ion pore behaviour in the CFTR chloride channel. Nature 366, 79-82.
20. VENGLARIK, C. J., SINGH, A. K. & BRIDGES, R. J. (1994). Comparison of -nitro versus -amino 4,4′-substituents of disulfonic stilbenes as chloride channel blockers. Molecular and Cellular Biochemistry 140, 137-146.
21. WOODHULL, A. M. (1973). Ionic blockage of sodium channels in nerve. Journal of General Physiology 61, 687-708.