-
1
-
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0035373775
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Force spectroscopy on single passive biomolecules and single biomolecular bonds
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A comprehensive review including discussions of the basic physics of the experimental techniques used to measure single-molecule mechanics and the theory underlying the energy landscape model. The applications discussed are stretching of single DNA molecules, force-induced dissociation of single bonds and unfolding of multidomain proteins.
-
Merkel R. Force spectroscopy on single passive biomolecules and single biomolecular bonds. Phys. Rep. Rev. Sect. Phys. Lett. 346:2001;344-385 A comprehensive review including discussions of the basic physics of the experimental techniques used to measure single-molecule mechanics and the theory underlying the energy landscape model. The applications discussed are stretching of single DNA molecules, force-induced dissociation of single bonds and unfolding of multidomain proteins.
-
(2001)
Phys. Rep. Rev. Sect. Phys. Lett.
, vol.346
, pp. 344-385
-
-
Merkel, R.1
-
4
-
-
0001160952
-
Ligand-receptor interactions
-
Bongrand P. Ligand-receptor interactions. Rep. Prog. Phys. 62:1999;921-968.
-
(1999)
Rep. Prog. Phys.
, vol.62
, pp. 921-968
-
-
Bongrand, P.1
-
6
-
-
0018101150
-
Models for the specific adhesion of cells to cells
-
Bell G.I. Models for the specific adhesion of cells to cells. Science. 200:1978;618-627.
-
(1978)
Science
, vol.200
, pp. 618-627
-
-
Bell, G.I.1
-
7
-
-
0034979287
-
Probing the relation between force-lifetime-and chemistry in single molecular bonds
-
This review covers current theory of dynamic force spectroscopy studies in combination with experimental data on force-induced receptor-ligand unbinding.
-
Evans E. Probing the relation between force-lifetime-and chemistry in single molecular bonds. Annu. Rev. Biophys. Biomol. Struct. 30:2001;105-128 This review covers current theory of dynamic force spectroscopy studies in combination with experimental data on force-induced receptor-ligand unbinding.
-
(2001)
Annu. Rev. Biophys. Biomol. Struct.
, vol.30
, pp. 105-128
-
-
Evans, E.1
-
8
-
-
41349113512
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Dynamic response of adhesion complexes: Beyond the single-path picture
-
A theoretical analysis of single-molecule bonds with complex energy landscapes, showing that different landscapes may yield the same dynamic force spectra.
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Bartolo D., Derenyi I., Ajdari A. Dynamic response of adhesion complexes: beyond the single-path picture. Phys. Rev. E. 65:2002;051910 A theoretical analysis of single-molecule bonds with complex energy landscapes, showing that different landscapes may yield the same dynamic force spectra.
-
(2002)
Phys. Rev. E.
, vol.65
, pp. 051910
-
-
Bartolo, D.1
Derenyi, I.2
Ajdari, A.3
-
9
-
-
0035957434
-
Free energy reconstruction from nonequilibrium single-molecule pulling experiments
-
The authors show how equilibrium free energy profiles can be extracted from pulling force measurements using the formalism of Jarzynski [10].
-
Hummer G., Szabo A. Free energy reconstruction from nonequilibrium single-molecule pulling experiments. Proc. Natl. Acad. Sci. USA. 98:2001;3658-3661 The authors show how equilibrium free energy profiles can be extracted from pulling force measurements using the formalism of Jarzynski [10].
-
(2001)
Proc. Natl. Acad. Sci. USA
, vol.98
, pp. 3658-3661
-
-
Hummer, G.1
Szabo, A.2
-
10
-
-
4243754128
-
Nonequilibrium equality for free energy differences
-
Jarzynski C. Nonequilibrium equality for free energy differences. Phys. Rev. Lett. 78:1997;2690-2693.
-
(1997)
Phys. Rev. Lett.
, vol.78
, pp. 2690-2693
-
-
Jarzynski, C.1
-
11
-
-
0037036070
-
Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski's equality
-
Jarzynski's theory [10] was tested for the first time by mechanically unfolding single RNA molecules, thus providing an experimental bridge between equilibrium and nonequilibrium processes.
-
Liphardt J., Dumont S., Smith S.B., Tinoco I. Jr., Bustamante C. Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski's equality. Science. 296:2002;1832-1835 Jarzynski's theory [10] was tested for the first time by mechanically unfolding single RNA molecules, thus providing an experimental bridge between equilibrium and nonequilibrium processes.
-
(2002)
Science
, vol.296
, pp. 1832-1835
-
-
Liphardt, J.1
Dumont, S.2
Smith, S.B.3
Tinoco I., Jr.4
Bustamante, C.5
-
12
-
-
0035312645
-
Steered molecular dynamics and mechanical functions of proteins
-
Isralewitz B., Gao M., Schulten K. Steered molecular dynamics and mechanical functions of proteins. Curr. Opin. Struct. Biol. 11:2001;224-230.
-
(2001)
Curr. Opin. Struct. Biol.
, vol.11
, pp. 224-230
-
-
Isralewitz, B.1
Gao, M.2
Schulten, K.3
-
13
-
-
0034633765
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The state diagram for cell adhesion under flow: Leukocyte rolling and firm adhesion
-
Different adhesion behaviors of leukocytes were modeled as a function of the most important molecular properties of individual receptor-ligand pairs. It was shown that the unstressed dissociation rate and the bond interaction length are most important for control of the dynamics of cell adhesion. The simulation model described provides the means to elucidate how the structures of receptor and ligand molecules correlate with their dynamic properties and receptor-mediated cell adhesion.
-
Chang K.C., Tees D.F., Hammer D.A. The state diagram for cell adhesion under flow: leukocyte rolling and firm adhesion. Proc. Natl. Acad Sci. USA. 97:2000;11262-11267 Different adhesion behaviors of leukocytes were modeled as a function of the most important molecular properties of individual receptor-ligand pairs. It was shown that the unstressed dissociation rate and the bond interaction length are most important for control of the dynamics of cell adhesion. The simulation model described provides the means to elucidate how the structures of receptor and ligand molecules correlate with their dynamic properties and receptor-mediated cell adhesion.
-
(2000)
Proc. Natl. Acad Sci. USA
, vol.97
, pp. 11262-11267
-
-
Chang, K.C.1
Tees, D.F.2
Hammer, D.A.3
-
14
-
-
0342350248
-
Dynamic force spectroscopy of molecular adhesion bonds
-
Heymann B., Grubmuller H. Dynamic force spectroscopy of molecular adhesion bonds. Phys. Rev. Lett. 84:2000;6126-6129.
-
(2000)
Phys. Rev. Lett.
, vol.84
, pp. 6126-6129
-
-
Heymann, B.1
Grubmuller, H.2
-
15
-
-
0036312338
-
Early mechanistic events in biotin dissociation from streptavidin
-
Hyre D.E., Amon L.M., Penzotti J.E., Le Trong I., Stenkamp R.E., Lybrand T.P., Stayton P.S. Early mechanistic events in biotin dissociation from streptavidin. Nat. Struct. Biol. 9:2002;582-585.
-
(2002)
Nat. Struct. Biol.
, vol.9
, pp. 582-585
-
-
Hyre, D.E.1
Amon, L.M.2
Penzotti, J.E.3
Le Trong, I.4
Stenkamp, R.E.5
Lybrand, T.P.6
Stayton, P.S.7
-
16
-
-
0036371130
-
Single-molecule force measurements
-
Edited by Jena BP, Horber JKH. Academic Press
-
Chen AL, Moy VT: Single-molecule force measurements. In Atomic Force Microscopy in Cell Biology. Edited by Jena BP, Horber JKH. Academic Press; 2002:301-309.
-
(2002)
Atomic Force Microscopy in Cell Biology
, pp. 301-309
-
-
Chen, A.L.1
Moy, V.T.2
-
17
-
-
0030924943
-
Optical trapping and manipulation of neutral particles using lasers
-
Ashkin A. Optical trapping and manipulation of neutral particles using lasers. Proc. Natl. Acad Sci. USA. 94:1997;4852-4860.
-
(1997)
Proc. Natl. Acad Sci. USA
, vol.94
, pp. 4852-4860
-
-
Ashkin, A.1
-
18
-
-
0029062769
-
Sensitive technique to probe molecular adhesion and structural linkages at biological interfaces
-
Evans E., Ritchie K., Merkel R. Sensitive technique to probe molecular adhesion and structural linkages at biological interfaces. Biophys. J. 68:1995;2580-2587.
-
(1995)
Biophys. J.
, vol.68
, pp. 2580-2587
-
-
Evans, E.1
Ritchie, K.2
Merkel, R.3
-
19
-
-
0034091670
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Time and force dependence of the rupture of glycoprotein IIb-IIIa-fibrinogen bonds between latex spheres
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The breaking force of fibrinogen-integrin bonds was shown to be directly dependent on the time of bond formation.
-
Goldsmith H.L., McIntosh F.A., Shahin J., Frojmovic M.M. Time and force dependence of the rupture of glycoprotein IIb-IIIa-fibrinogen bonds between latex spheres. Biophys. J. 78:2000;1195-1206 The breaking force of fibrinogen-integrin bonds was shown to be directly dependent on the time of bond formation.
-
(2000)
Biophys. J.
, vol.78
, pp. 1195-1206
-
-
Goldsmith, H.L.1
McIntosh, F.A.2
Shahin, J.3
Frojmovic, M.M.4
-
20
-
-
0033548611
-
How strong is a covalent bond?
-
Grandbois M., Beyer M., Rief M., Clausen-Schaumann H., Gaub H.E. How strong is a covalent bond? Science. 283:1999;1727-1730.
-
(1999)
Science
, vol.283
, pp. 1727-1730
-
-
Grandbois, M.1
Beyer, M.2
Rief, M.3
Clausen-Schaumann, H.4
Gaub, H.E.5
-
21
-
-
0035996985
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Multi-bead-and-spring model to interpret protein detachment studied by AFM force spectroscopy
-
Gergely C., Hemmerle J., Schaaf P., Horber J.K., Voegel J.C., Senger B. Multi-bead-and-spring model to interpret protein detachment studied by AFM force spectroscopy. Biophys. J. 83:2002;706-722.
-
(2002)
Biophys. J.
, vol.83
, pp. 706-722
-
-
Gergely, C.1
Hemmerle, J.2
Schaaf, P.3
Horber, J.K.4
Voegel, J.C.5
Senger, B.6
-
22
-
-
0037188509
-
Binding strength and activation state of single fibrinogen-integrin pairs on living cells
-
3 on live platelets. The probability of effective binding events was directly proportional to the degree of cell stimulation but the adhesion strength was not changed, indicating that platelets regulate the number of accessible binding sites, not the affinity of ligand binding.
-
3 on live platelets. The probability of effective binding events was directly proportional to the degree of cell stimulation but the adhesion strength was not changed, indicating that platelets regulate the number of accessible binding sites, not the affinity of ligand binding.
-
(2002)
Proc. Natl. Acad Sci. USA
, vol.99
, pp. 7426-7431
-
-
Litvinov, R.I.1
Shuman, H.2
Bennett, J.S.3
Weisel, J.W.4
-
23
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-
0033166686
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The influence of epitope availability on atomic-force microscope studies of antigen-antibody interactions
-
Allen S., Davies J., Davies M.C., Dawkes A.C., Roberts C.J., Tendler S.J., Williams P.M. The influence of epitope availability on atomic-force microscope studies of antigen-antibody interactions. Biochem J. 341:1999;173-178.
-
(1999)
Biochem J.
, vol.341
, pp. 173-178
-
-
Allen, S.1
Davies, J.2
Davies, M.C.3
Dawkes, A.C.4
Roberts, C.J.5
Tendler, S.J.6
Williams, P.M.7
-
24
-
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0034636032
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Cadherin interaction probed by atomic force microscopy
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The unbinding force of a VE-cadherin dimer was shown to increase with increasing encounter duration, suggesting spontaneous lateral association of VE-cadherins into complexes with cumulative binding strength.
-
Baumgartner W., Hinterdorfer P., Ness W., Raab A., Vestweber D., Schindler H., Drenckhahn D. Cadherin interaction probed by atomic force microscopy. Proc. Natl. Acad Sci. USA. 97:2000;4005-4010 The unbinding force of a VE-cadherin dimer was shown to increase with increasing encounter duration, suggesting spontaneous lateral association of VE-cadherins into complexes with cumulative binding strength.
-
(2000)
Proc. Natl. Acad Sci. USA
, vol.97
, pp. 4005-4010
-
-
Baumgartner, W.1
Hinterdorfer, P.2
Ness, W.3
Raab, A.4
Vestweber, D.5
Schindler, H.6
Drenckhahn, D.7
-
25
-
-
0001694842
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Statistical breakage of single protein A-IgG bonds reveals crossover from spontaneous to force-induced bond dissociation
-
Simson D.A., Strigl M., Hohenadl M., Merkel R. Statistical breakage of single protein A-IgG bonds reveals crossover from spontaneous to force-induced bond dissociation. Phys. Rev. Lett. 83:1999;652-655.
-
(1999)
Phys. Rev. Lett.
, vol.83
, pp. 652-655
-
-
Simson, D.A.1
Strigl, M.2
Hohenadl, M.3
Merkel, R.4
-
26
-
-
0031784238
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Simultaneous height and adhesion imaging of antibody-antigen interactions by atomic force microscopy
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Willemsen O.H., Snel M.M., van der Werf K.O., de Grooth B.G., Greve J., Hinterdorfer P., Gruber H.J., Schindler H., van Kooyk Y., Figdor C.G. Simultaneous height and adhesion imaging of antibody-antigen interactions by atomic force microscopy. Biophys. J. 75:1998;2220-2228.
-
(1998)
Biophys. J.
, vol.75
, pp. 2220-2228
-
-
Willemsen, O.H.1
Snel, M.M.2
Van Der Werf, K.O.3
De Grooth, B.G.4
Greve, J.5
Hinterdorfer, P.6
Gruber, H.J.7
Schindler, H.8
Van Kooyk, Y.9
Figdor, C.G.10
-
27
-
-
0035016898
-
Detection and characterization of individual intermolecular bonds using optical tweezers
-
Stout A.L. Detection and characterization of individual intermolecular bonds using optical tweezers. Biophys. J. 80:2001;2976-2986.
-
(2001)
Biophys. J.
, vol.80
, pp. 2976-2986
-
-
Stout, A.L.1
-
29
-
-
0035986935
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Measuring receptor/ligand interaction at the single-bond level: Experimental and interpretative issues
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Different approaches that have been used to prove single-molecule interactions are summarized and analyzed. It is concluded that no single criterion is sufficient for formal proof that single binding events are actually observed, but the cumulative body of evidence may provide reasonable confidence.
-
Zhu C., Long M., Chesla S.E., Bongrand P. Measuring receptor/ligand interaction at the single-bond level: experimental and interpretative issues. Ann. Biomed. Eng. 30:2002;305-314 Different approaches that have been used to prove single-molecule interactions are summarized and analyzed. It is concluded that no single criterion is sufficient for formal proof that single binding events are actually observed, but the cumulative body of evidence may provide reasonable confidence.
-
(2002)
Ann. Biomed. Eng.
, vol.30
, pp. 305-314
-
-
Zhu, C.1
Long, M.2
Chesla, S.E.3
Bongrand, P.4
-
30
-
-
0033050306
-
2 integrins, and CD45 to neutrophil cytoskeleton and membrane
-
2 integrins, and CD45 to neutrophil cytoskeleton and membrane. Biophys. J. 77:1999;587-596.
-
(1999)
Biophys. J.
, vol.77
, pp. 587-596
-
-
Shao, J.Y.1
Hochmuth, R.M.2
-
31
-
-
33744834556
-
Dynamic strength of molecular anchoring and material cohesion in fluid biomembranes
-
Evans E., Ludwig F. Dynamic strength of molecular anchoring and material cohesion in fluid biomembranes. J. Phys. Condens. Matter. 12:2000;A315-A320.
-
(2000)
J. Phys. Condens. Matter
, vol.12
-
-
Evans, E.1
Ludwig, F.2
-
32
-
-
0036382932
-
Micromechanical coupling between cell surface receptors and RGD peptides
-
Rahman A., Tseng Y., Wirtz D. Micromechanical coupling between cell surface receptors and RGD peptides. Biochem. Biophys. Res. Commun. 296:2002;771-778.
-
(2002)
Biochem. Biophys. Res. Commun.
, vol.296
, pp. 771-778
-
-
Rahman, A.1
Tseng, Y.2
Wirtz, D.3
-
33
-
-
0034043222
-
Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion
-
Chen A., Moy V.T. Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion. Biophys. J. 78:2000;2814-2820.
-
(2000)
Biophys. J.
, vol.78
, pp. 2814-2820
-
-
Chen, A.1
Moy, V.T.2
-
34
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0036538656
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Co-regulation of cell adhesion by nanoscale RGD organization and mechanical stimulus
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1 were allowed to bind and then detach from surfaces coated with clustered RGD peptide. Instead of the expected decrease in the number of cells with increasing force, the fraction of cells remaining attached increased as the pulling force was increased to between 70 and 150 pN. This adhesion reinforcement directly correlated with the cluster size and was not observed on fibronectin-coated surfaces.
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1 were allowed to bind and then detach from surfaces coated with clustered RGD peptide. Instead of the expected decrease in the number of cells with increasing force, the fraction of cells remaining attached increased as the pulling force was increased to between 70 and 150 pN. This adhesion reinforcement directly correlated with the cluster size and was not observed on fibronectin-coated surfaces.
-
(2002)
J. Cell Sci.
, vol.115
, pp. 1423-1433
-
-
Koo, L.Y.1
Irvine, D.J.2
Mayes, A.M.3
Lauffenburger, D.A.4
Griffith, L.G.5
-
35
-
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0035964311
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Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow
-
Ramachandran V., Yago T., Epperson T.K., Kobzdej M.M., Nollert M.U., Cummings R.D., Zhu C., McEver R.P. Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow. Proc. Natl. Acad Sci. USA. 98:2001;10166-10171.
-
(2001)
Proc. Natl. Acad Sci. USA
, vol.98
, pp. 10166-10171
-
-
Ramachandran, V.1
Yago, T.2
Epperson, T.K.3
Kobzdej, M.M.4
Nollert, M.U.5
Cummings, R.D.6
Zhu, C.7
McEver, R.P.8
-
36
-
-
0034575444
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Cell mechanics: Mechanical response, cell adhesion, and molecular deformation
-
Zhu C., Gang B., Wang N. Cell mechanics: mechanical response, cell adhesion, and molecular deformation. Annu. Rev. Biomed. Eng. 2:2000;189-226.
-
(2000)
Annu. Rev. Biomed. Eng.
, vol.2
, pp. 189-226
-
-
Zhu, C.1
Gang, B.2
Wang, N.3
-
37
-
-
0035426369
-
Molecular properties in cell adhesion: A physical and engineering perspective
-
Orsello C.E., Lauffenburger D.A., Hammer D.A. Molecular properties in cell adhesion: a physical and engineering perspective. Trends Biotechnol. 19:2001;310-316.
-
(2001)
Trends Biotechnol.
, vol.19
, pp. 310-316
-
-
Orsello, C.E.1
Lauffenburger, D.A.2
Hammer, D.A.3
-
38
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0036286198
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Selectin-like kinetics and biomechanics promote rapid platelet adhesion in flow: The GPIb(alpha)-vWF tether bond
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off, but no significant alteration in the ability of the tether bond to resist shear-induced forces.
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off, but no significant alteration in the ability of the tether bond to resist shear-induced forces.
-
(2002)
Biophys. J.
, vol.83
, pp. 194-205
-
-
Doggett, T.A.1
Girdhar, G.2
Lawshe, A.3
Schmidtke, D.W.4
Laurenzi, I.J.5
Diamond, S.L.6
Diacovo, T.G.7
-
40
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0036789541
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Force spectroscopy of the leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction
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Cells expressing LFA-1 were immobilized on AFM tips via concanavalin A (ConA) and interacted with ICAM-1 expressed by fibroblasts or physically adsorbed to the surface of a tissue culture dish in a soluble truncated form. The results of rupture force measurements at loading rates covering three orders of magnitude revealed two loading regimes of different physiological significance.
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Zhang X., Wojcikiewicz E., Moy V.T. Force spectroscopy of the leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction. Biophys. J. 83:2002;2270-2279 Cells expressing LFA-1 were immobilized on AFM tips via concanavalin A (ConA) and interacted with ICAM-1 expressed by fibroblasts or physically adsorbed to the surface of a tissue culture dish in a soluble truncated form. The results of rupture force measurements at loading rates covering three orders of magnitude revealed two loading regimes of different physiological significance.
-
(2002)
Biophys. J.
, vol.83
, pp. 2270-2279
-
-
Zhang, X.1
Wojcikiewicz, E.2
Moy, V.T.3
-
41
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0037306230
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1 integrin-fibronectin interaction
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1 were attached to ConA-coated AFM tips and touched repeatedly to a surface bearing a fragment derived from the cell-binding fibronectin domain. Two force loading regimes, slow and fast, obtained with a loading rate range of three orders of magnitude, had different susceptibility to deletions in fibronectin.
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1 were attached to ConA-coated AFM tips and touched repeatedly to a surface bearing a fragment derived from the cell-binding fibronectin domain. Two force loading regimes, slow and fast, obtained with a loading rate range of three orders of magnitude, had different susceptibility to deletions in fibronectin.
-
(2003)
Biophys. J.
, vol.84
, pp. 1-11
-
-
Li, F.1
Redick, S.D.2
Erickson, H.P.3
Moy, V.T.4
-
42
-
-
0032763190
-
A direct comparison of selectin-mediated transient, adhesive events using high temporal resolution
-
Smith M.J., Berg E.L., Lawrence M.B. A direct comparison of selectin-mediated transient, adhesive events using high temporal resolution. Biophys. J. 77:1999;3371-3383.
-
(1999)
Biophys. J.
, vol.77
, pp. 3371-3383
-
-
Smith, M.J.1
Berg, E.L.2
Lawrence, M.B.3
-
43
-
-
0035970109
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Selectin receptor-ligand bonds: Formation limited by shear rate and dissociation governed by the Bell model
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Neutrophils were rolled over a P-selectin-coated surface at different shear rates and shear stresses (regulated by viscosity at the same shear rate). Bond formation between neutrophils and the surface was a function of shear rate, whereas bond dissociation was a function of shear stress and hence the force on the bond. The Bell model was shown to fit well the experimental parameters of unbinding.
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Chen S., Springer T.A. Selectin receptor-ligand bonds: formation limited by shear rate and dissociation governed by the Bell model. Proc. Natl. Acad Sci. USA. 98:2001;950-955 Neutrophils were rolled over a P-selectin-coated surface at different shear rates and shear stresses (regulated by viscosity at the same shear rate). Bond formation between neutrophils and the surface was a function of shear rate, whereas bond dissociation was a function of shear stress and hence the force on the bond. The Bell model was shown to fit well the experimental parameters of unbinding.
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(2001)
Proc. Natl. Acad Sci. USA
, vol.98
, pp. 950-955
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Chen, S.1
Springer, T.A.2
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44
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0013198341
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Single-molecule characterization of P-selectin/ligand binding
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in press
-
Hanley W, McCarty O, Jadhav S, Tseng Y, Wirtz D, Konstantopoulos K: Single-molecule characterization of P-selectin/ligand binding. J Biol Chem 2003, in press.
-
(2003)
J Biol Chem
-
-
Hanley, W.1
McCarty, O.2
Jadhav, S.3
Tseng, Y.4
Wirtz, D.5
Konstantopoulos, K.6
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45
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0036231595
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Single molecule recognition of protein binding epitopes in brush border membranes by force microscopy
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The observed specific recognition signals with anti-γ-glutamyl-transpeptidase (γ-GT) antibody proved the accessibility of γ-GT and were indicative of proper membrane orientation in the vesicles. Then, the normal unbinding force profile for SGCT and its antibody was determined; it changed in the presence of either D-glucose or phlorizine, a specific inhibitor of SGCT. The data demonstrate that the specific antibody-binding epitope of the SGCT, located in the segment comprising amino acids 606-630, undergoes conformational changes during D-glucose transport.
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Wielert-Badt S., Hinterdorfer P., Gruber H.J., Lin J.T., Badt D., Wimmer B., Schindler H., Kinne R.K. Single molecule recognition of protein binding epitopes in brush border membranes by force microscopy. Biophys. J. 82:2002;2767-2774 The observed specific recognition signals with anti-γ-glutamyl-transpeptidase (γ-GT) antibody proved the accessibility of γ-GT and were indicative of proper membrane orientation in the vesicles. Then, the normal unbinding force profile for SGCT and its antibody was determined; it changed in the presence of either D-glucose or phlorizine, a specific inhibitor of SGCT. The data demonstrate that the specific antibody-binding epitope of the SGCT, located in the segment comprising amino acids 606-630, undergoes conformational changes during D-glucose transport.
-
(2002)
Biophys. J.
, vol.82
, pp. 2767-2774
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Wielert-Badt, S.1
Hinterdorfer, P.2
Gruber, H.J.3
Lin, J.T.4
Badt, D.5
Wimmer, B.6
Schindler, H.7
Kinne, R.K.8
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46
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0033884332
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Characterization of single actomyosin rigor bonds: Load dependence of lifetime and mechanical properties
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Bead-coupled actin filaments were attached at two or more points to a surface coated with heavy meromyosin (HMM) and then pulled via the optically trapped latex bead to disrupt the bond that was the closest to the bead. The measured average unbinding force of the actin-HMM rigor bond in the absence of ATP was a few times larger than the known sliding force.
-
Nishizaka T., Seo R., Tadakuma H., Kinosita K. Jr., Ishiwata S. Characterization of single actomyosin rigor bonds: load dependence of lifetime and mechanical properties. Biophys. J. 79:2000;962-974 Bead-coupled actin filaments were attached at two or more points to a surface coated with heavy meromyosin (HMM) and then pulled via the optically trapped latex bead to disrupt the bond that was the closest to the bead. The measured average unbinding force of the actin-HMM rigor bond in the absence of ATP was a few times larger than the known sliding force.
-
(2000)
Biophys. J.
, vol.79
, pp. 962-974
-
-
Nishizaka, T.1
Seo, R.2
Tadakuma, H.3
Kinosita K., Jr.4
Ishiwata, S.5
-
47
-
-
0034051267
-
Temperature change does not affect force between single actin filaments and HMM from rabbit muscles
-
Kawai M., Kawaguchi K., Saito M., Ishiwata S. Temperature change does not affect force between single actin filaments and HMM from rabbit muscles. Biophys. J. 78:2000;3112-3119.
-
(2000)
Biophys. J.
, vol.78
, pp. 3112-3119
-
-
Kawai, M.1
Kawaguchi, K.2
Saito, M.3
Ishiwata, S.4
-
48
-
-
0036078426
-
Visualization and force measurement of branching by Arp2/3 complex and N-WASP in actin filament
-
The Arp2/3 protein complex and WASP family proteins containing the active VCA domain are involved in regulation of actin polymerization. Bead-attached actin filaments and free Arp2/3 were allowed to reach the VCA-coated surface, with subsequent unbinding induced by pulling the optically trapped bead and controlling its displacement. It was suggested that the measured unbinding force reflected the detachment of the actin-Arp2/3 complex from the VCA-coated surface, rather than the rupture of actin-Arp2/3 bonds.
-
Fujiwara I., Suetsugu S., Uemura S., Takenawa T., Ishiwata S. Visualization and force measurement of branching by Arp2/3 complex and N-WASP in actin filament. Biochem. Biophys. Res. Commun. 293:2002;1550-1555 The Arp2/3 protein complex and WASP family proteins containing the active VCA domain are involved in regulation of actin polymerization. Bead-attached actin filaments and free Arp2/3 were allowed to reach the VCA-coated surface, with subsequent unbinding induced by pulling the optically trapped bead and controlling its displacement. It was suggested that the measured unbinding force reflected the detachment of the actin-Arp2/3 complex from the VCA-coated surface, rather than the rupture of actin-Arp2/3 bonds.
-
(2002)
Biochem. Biophys. Res. Commun.
, vol.293
, pp. 1550-1555
-
-
Fujiwara, I.1
Suetsugu, S.2
Uemura, S.3
Takenawa, T.4
Ishiwata, S.5
-
49
-
-
0037197984
-
Kinesin-microtubule binding depends on both nucleotide state and loading direction
-
The rupture force distribution for microtubules and two-headed kinesin, unlike that for one-headed kinesin, was bimodal in the presence of an ATP analog, with the larger values representing double-headed binding. The results obtained in the presence of ADP and in a nucleotide-free state were analyzed with regard to the modern mechanistic model of kinesin motility.
-
Uemura S., Kawaguchi K., Yajima J., Edamatsu M., Toyoshima Y.Y., Ishiwata S. Kinesin-microtubule binding depends on both nucleotide state and loading direction. Proc. Natl. Acad Sci. USA. 99:2002;5977-5981 The rupture force distribution for microtubules and two-headed kinesin, unlike that for one-headed kinesin, was bimodal in the presence of an ATP analog, with the larger values representing double-headed binding. The results obtained in the presence of ADP and in a nucleotide-free state were analyzed with regard to the modern mechanistic model of kinesin motility.
-
(2002)
Proc. Natl. Acad Sci. USA
, vol.99
, pp. 5977-5981
-
-
Uemura, S.1
Kawaguchi, K.2
Yajima, J.3
Edamatsu, M.4
Toyoshima, Y.Y.5
Ishiwata, S.6
-
50
-
-
0033575007
-
Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy
-
Lehenkari P.P., Horton M.A. Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 259:1999;645-650.
-
(1999)
Biochem. Biophys. Res. Commun.
, vol.259
, pp. 645-650
-
-
Lehenkari, P.P.1
Horton, M.A.2
-
51
-
-
0036624844
-
Ultralarge multimers of von Willebrand factor form spontaneous high- strength bonds with the platelet glycoprotein Ib-IX complex: Studies using optical tweezers
-
Arya M., Anvari B., Romo G.M., Cruz M.A., Dong J.F., McIntire L.V., Moake J.L., Lopez J.A. Ultralarge multimers of von Willebrand factor form spontaneous high- strength bonds with the platelet glycoprotein Ib-IX complex: studies using optical tweezers. Blood. 99:2002;3971-3977.
-
(2002)
Blood
, vol.99
, pp. 3971-3977
-
-
Arya, M.1
Anvari, B.2
Romo, G.M.3
Cruz, M.A.4
Dong, J.F.5
McIntire, L.V.6
Moake, J.L.7
Lopez, J.A.8
-
52
-
-
0032514687
-
Force-mediated kinetics of single P-selectin/ligand complexes by atomic force microscopy
-
Fritz J., Katopodis A.G., Kolbinger F., Anselmetti D. Force-mediated kinetics of single P-selectin/ligand complexes by atomic force microscopy. Proc. Natl. Acad Sci. USA. 95:1998;12783-122288.
-
(1998)
Proc. Natl. Acad Sci. USA
, vol.95
, pp. 12783-122288
-
-
Fritz, J.1
Katopodis, A.G.2
Kolbinger, F.3
Anselmetti, D.4
-
53
-
-
0033802766
-
Short-term binding of fibroblasts to fibronectin: Optical tweezers experiments and probabilistic analysis
-
Thoumine O., Kocian P., Kottelat A., Meister J.J. Short-term binding of fibroblasts to fibronectin: optical tweezers experiments and probabilistic analysis. Eur. Biophys. J. 29:2000;398-408.
-
(2000)
Eur. Biophys. J.
, vol.29
, pp. 398-408
-
-
Thoumine, O.1
Kocian, P.2
Kottelat, A.3
Meister, J.J.4
-
54
-
-
0033772174
-
Discrete interactions in cell adhesion measured by single-molecule force spectroscopy
-
Benoit M., Gabriel D., Gerisch G., Gaub H.E. Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat. Cell Biol. 2:2000;313-317.
-
(2000)
Nat. Cell Biol.
, vol.2
, pp. 313-317
-
-
Benoit, M.1
Gabriel, D.2
Gerisch, G.3
Gaub, H.E.4
-
56
-
-
0030952393
-
Detection of antigen-antibody binding events with the atomic force microscope
-
Allen S., Chen X., Davies J., Davies M.C., Dawkes A.C., Edwards J.C., Roberts C.J., Sefton J., Tendler S.J., Williams P.M. Detection of antigen-antibody binding events with the atomic force microscope. Biochemistry. 36:1997;7457-7463.
-
(1997)
Biochemistry
, vol.36
, pp. 7457-7463
-
-
Allen, S.1
Chen, X.2
Davies, J.3
Davies, M.C.4
Dawkes, A.C.5
Edwards, J.C.6
Roberts, C.J.7
Sefton, J.8
Tendler, S.J.9
Williams, P.M.10
-
57
-
-
0029883621
-
Detection and localization of individual antibody-antigen recognition events by atomic force microscopy
-
Hinterdorfer P., Baumgartner W., Gruber H.J., Schilcher K., Schindler H. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proc. Natl. Acad Sci. USA. 93:1996;3477-3481.
-
(1996)
Proc. Natl. Acad Sci. USA
, vol.93
, pp. 3477-3481
-
-
Hinterdorfer, P.1
Baumgartner, W.2
Gruber, H.J.3
Schilcher, K.4
Schindler, H.5
-
58
-
-
0035138892
-
2+ release channel (RYR1)
-
2+ release channel (RYR1). Ultramicroscopy. 86:2001;129-137.
-
(2001)
Ultramicroscopy
, vol.86
, pp. 129-137
-
-
Kada, G.1
Blayney, L.2
Jeyakumar, L.H.3
Kienberger, F.4
Pastushenko, V.P.5
Fleischer, S.6
Schindler, H.7
Lai, F.A.8
Hinterdorfer, P.9
-
59
-
-
0034669526
-
Differences in zero-force and force-driven kinetics of ligand dissociation from beta-galactoside-specific proteins (plant and animal lectins, immunoglobulin G) monitored by plasmon resonance and dynamic single molecule force microscopy
-
Dettmann W., Grandbois M., Andre S., Benoit M., Wehle A.K., Kaltner H., Gabius H.J., Gaub H.E. Differences in zero-force and force-driven kinetics of ligand dissociation from beta-galactoside-specific proteins (plant and animal lectins, immunoglobulin G) monitored by plasmon resonance and dynamic single molecule force microscopy. Arch. Biochem. Biophys. 383:2000;157-170.
-
(2000)
Arch. Biochem. Biophys.
, vol.383
, pp. 157-170
-
-
Dettmann, W.1
Grandbois, M.2
Andre, S.3
Benoit, M.4
Wehle, A.K.5
Kaltner, H.6
Gabius, H.J.7
Gaub, H.E.8
-
60
-
-
0030992359
-
Scanning force microscopy of the interaction events between a single molecule of heavy meromyosin and actin
-
Nakajima H., Kunioka Y., Nakano K., Shimizu K., Seto M., Ando T. Scanning force microscopy of the interaction events between a single molecule of heavy meromyosin and actin. Biochem. Biophys. Res. Commun. 234:1997;178-182.
-
(1997)
Biochem. Biophys. Res. Commun.
, vol.234
, pp. 178-182
-
-
Nakajima, H.1
Kunioka, Y.2
Nakano, K.3
Shimizu, K.4
Seto, M.5
Ando, T.6
-
61
-
-
0032554643
-
Direct force measurements of insulin monomer-monomer interactions
-
Yip C.M., Yip C.C., Ward M.D. Direct force measurements of insulin monomer-monomer interactions. Biochemistry. 37:1998;5439-5449.
-
(1998)
Biochemistry
, vol.37
, pp. 5439-5449
-
-
Yip, C.M.1
Yip, C.C.2
Ward, M.D.3
-
62
-
-
0031835964
-
Atomic force microscopy detects changes in the interaction forces between GroEL and substrate proteins
-
Vinckier A., Gervasoni P., Zaugg F., Ziegler U., Lindner P., Groscurth P., Pluckthun A., Semenza G. Atomic force microscopy detects changes in the interaction forces between GroEL and substrate proteins. Biophys. J. 74:1998;3256-3263.
-
(1998)
Biophys. J.
, vol.74
, pp. 3256-3263
-
-
Vinckier, A.1
Gervasoni, P.2
Zaugg, F.3
Ziegler, U.4
Lindner, P.5
Groscurth, P.6
Pluckthun, A.7
Semenza, G.8
-
63
-
-
0037306229
-
Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics
-
••], the authors proposed a model in which an equilibrium exists between single- and double-headed binding, and detachment from the microtubule occurs during either single- or double-headed binding. They conclude that the double-headed binding state is predominant at equilibrium in the absence of load not only in the AMP-PNP state but also in the nucleotide-free state.
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••], the authors proposed a model in which an equilibrium exists between single- and double-headed binding, and detachment from the microtubule occurs during either single- or double-headed binding. They conclude that the double-headed binding state is predominant at equilibrium in the absence of load not only in the AMP-PNP state but also in the nucleotide-free state.
-
(2003)
Biophys. J.
, vol.84
, pp. 1103-1113
-
-
Kawaguchi, K.1
Uemura, S.2
Ishiwata, S.3
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