Focal volume optics and experimental artifacts in confocal fluorescence correlation spectroscopy

Biophysical Journal

Volumn 83, Issue 4, 2002, Pages 2300-2317

  Hess, Samuel T ^a   Webb, Watt W ^a,b  

^a Cornell University   (United States)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ARTIFACT; CONFOCAL FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE SPECTROSCOPY; ILLUMINATION; OPTICS; SIGNAL NOISE RATIO;

MUS;

EID: 0036789423     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)73990-8     Document Type: Article

References (43)

1. Born, M., and E. Wolf. 1991. Principles of Optics, 6th Ed. Pergamon Press, Oxford. 484.
2. Brock, R., M. A. Hink, and T. M. Jovin. 1998. Fluorescence correlation microscopy of cells in the presence of autofluorescence. Biophys. J. 75:2547-2557.
3. Brock, R., G. Vamosi, G. Vereb, and T. M. Jovin. 1999. Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy. Proc. Natl. Acad. Sci. USA. 96:10123-10128.
4. Cluzel, P., M. Surette, and S. Leibler. 2000. An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells. Science. 287:1652-1655.
5. Cummins, H. Z., and H. L. Swinney. 1970. Light beating spectroscopy. Prog. in Optics. 8:135-200.
6. Eigen, M., and R. Rigler. 1994. Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc. Natl. Acad. Sci. USA. 91:5740-5747.
7. Elson, E. L., and D. Magde. 1974. Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers. 13:1-27.
8. Gu, M., and X. S. Gan. 1996. Effect of the detector size and the fluorescence wavelength on the resolution of three- and two-photon confocal microscopy. Bioimaging. 4:129-137.
9. Gu, M., and C. J. R. Sheppard. 1993. Effects of a finite-sized pinhole on 3D image-formation in confocal two-photon fluorescence microscopy. J. Mod. Optics. 40:2009-2024.
10. Hess, S. T., S. Huang, A. A. Heikal, and W. W. Webb. 2002. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry. 41:697-705.
11. Heikal, A. A., S. T. Hess, G. S. Baird, R. Y. Tsien, and W. W. Webb. 2000. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). Proc. Natl. Acad. Sci. USA. 97:11996-12001.
12. Hink, M. A., R. A. Griep, J. W. Borst, A. van Hoek, M. H. M. Eppink, A. Schots, and A. J. W. G. Visser. 2000. Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein. J. Biol. Chem. 275:17556-17560.
13. Kask, P., K. Palo, D. Ullmann, and K. Gall. 1999. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proc. Natl. Acad. Sci. USA. 96:13756-13761.
14. Koppel, D. E. 1974. Statistical accuracy in fluorescence correlation spectroscopy. Phys. Rev. A. 10:1938-1945.
15. Koppel, D. E., D. Axelrod, J. Schlessinger, E. L. Elson, and W. W. Webb. 1976. Dynamics of fluorescence marker concentration as a probe of mobility. Biophys. J. 16:1315-1329.
16. Magde, D., E. Elson, and W. W. Webb. 1972. Thermodynamic fluctuations in a reacting system measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
17. Magde, D., E. L. Elson, and W. W. Webb. 1974. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 13:29-61.
18. Maiti, S., U. Haupts, and W. W. Webb. 1997. Fluorescence correlation spectroscopy: diagnostics for sparse molecules. Proc. Natl. Acad. Sci. USA. 94:11753-11757.
19. Mertz, J., C. Xu, and W. W. Webb. 1995. Single molecule detection by two-photon-excited fluorescence. Optics Lett. 20:2532-2534.
20. Meseth, U., T. Wohland, R. Rigler, and H. Vogel. 1999. Resolution in fluorescence correlation measurements. Biophys. J. 76:1619-1631.
21. Politz, J. C., E. S. Browne, D. E. Wolf, and T. Pederson. 1998. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl. Acad. Sci. USA. 95:6043-6048.
22. Qian, H., and E. Elson. 1991. Analysis of confocal laser-microscope optics for 3-D fluorescence correlation spectroscopy. Appl. Optics. 30: 1185-1195.
23. Richards, B., and E. Wolf. 1959. Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system. Proc. R. Soc. Lond. Ser. A. 253:358-379.
24. Rigler, R., and E. S. Elson. 2001. Fluorescence correlation spectroscopy: theory and applications. Springer-Verlag, Berlin.
25. Rigler, R., and J. Widengren. 1990. Ultrasensitive detection of single molecules by fluorescence correlation spectroscopy. In BioScience. B. Klinge and C. Owman, editors. Lund University Press, Lund, Sweden. 180-183.
26. Rigler, R., Ü. Mets, J. Widengren, and P. Kask. 1993. Fluorescence correlation spectroscopy with high count rate and low background-analysis of translational diffusion. Eur. Biophys. J. 22:169-175.
27. Sandison, D. R., D. W. Piston, R. M. Williams, and W. W. Webb. 1995. Quantitative comparison of background rejection, signal-to-noise ratio, and resolution in confocal and full-field laser-scanning microscopies. Appl. Optics. 34:3576-3588.
28. Sandison, D. R., and W. W. Webb. 1994. Background rejection and signal-to-noise optimization in confocal and alternative fluorescence microscopes. Appl. Optics. 33:603-615.
29. Schneider, M. B., and W. W. Webb. 1981. Measurement of sub-micron laser-beam radii. Appl. Optics. 20:1382-1388.
30. Schwille, P., U. Haupts, S. Maiti, and W. W. Webb. 1999a. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys. J. 77:2251-2265.
31. Schwille, P., J. Korlach, and W. W. Webb. 1999b. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry. 36:176-182.
32. Schwille, P., F. Oehlenschlager, and N. G. Walter. 1996. Quantitative hybridization kinetics of DNA probes to RNA in solution followed by diffusional fluorescence correlation analysis. Biochemistry. 35: 10182-10193.
33. Self, S. A. 1983. Focusing of spherical Gaussian beams. Appl. Optics. 22:658-661.
34. Sheppard, C. J. R., C. J. Cogswell, and M. Gu. 1991. Signal strength and noise in confocal microscopy: factors influencing selection of an optimum detector aperture. Scanning. 13:233-240.
35. Sheppard, C. J. R., and H. J. Matthews. 1987. Imaging in high-aperture optical systems. J. Opt. Soc. Am. A. 4:1354-1360.
36. Thompson, N. L. 1991. Fluorescence correlation spectroscopy In Topics in Fluorescence Spectroscopy. Vol. 1. Techniques. J. R. Lakowicz, editor. Plenum Press, New York. 337-410.
37. Visser, N. V., M. A. Hink, A. van Hoek, and A. J. W. G. Visser. 1999. Comparison between fluorescence correlation spectroscopy and timeresolved fluorescence anisotropy as illustrated with a fluorescent dextran conjugate. J. Fluorescence. 9:251-255.
38. Wachsmuth, M., W. Waldeck, and J. Langowski. 2000. Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy. J. Mol. Biol. 298:677-689.
39. Webb, W. W. 1976. Applications of fluorescence correlation spectroscopy. Q. Rev. Biophys. 9:49-68.
40. Webb, W. W., K. S. Wells, D. R. Sandison, and J. H. Strickler. 1990. Criteria for quantitative dynamical confocal fluorescence imaging. In Optical Microscopy for Biology. B. Herman and K. Jacobson, editors. Wiley-Liss, New York. 73-108.
41. Wells, K. S., D. R. Sandison, J. H. Strickler, and W. W. Webb. 1990. Quantitative fluorescence imaging in laser scanning confocal microscopy. In Handbook of Biological Confocal Microscopy. J. Pawley, editor. Plenum Press, New York. 27-39.
42. Wolf, E. 1959. Electromagnetic diffraction in optical systems. I. An integral representation of the image field. Proc. R. Soc. Lond. Ser. A. 253:349-357.
43. Xu, C., and W. W. Webb. 1997. Multiphoton excitation of molecular fluorophores and nonlinear laser microscopy. In Topics in Fluorescence Spectroscopy, Vol. 5. J. R. Lakowicz, editor. Plenum Press, New York. 471-540.