Solute and macromolecule diffusion in cellular aqueous compartments

Trends in Biochemical Sciences

Volumn 27, Issue 1, 2002, Pages 27-33

  Verkman, Alan S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOTROPY; BLEACHING; CELL COMPARTMENTALIZATION; CELL MEMBRANE TRANSPORT; CELL METABOLISM; CELL ORGANELLE; CELL STRUCTURE; COMPARTMENT MODEL; CYTOPLASM; DIFFUSION; DRUG DELIVERY SYSTEM; ELECTRON TRANSPORT; ENDOPLASMIC RETICULUM; FLUORESCENCE; MACROMOLECULE; MICROSCOPY; MITOCHONDRIAL MEMBRANE; MOLECULAR DYNAMICS; MOLECULAR EVOLUTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVIEW; SIGNAL TRANSDUCTION; SOLUTE; VISCOSITY;

ANIMALS; BODY WATER; CELL PHYSIOLOGY; CYTOPLASM; DIFFUSION; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MACROMOLECULAR SUBSTANCES; ORGANELLES;

EID: 0036164351     PISSN: 09680004     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-0004(01)02003-5     Document Type: Review

References (40)

1. Inside a living cell
2. Macromolecular compartmentation and channeling
3. Implications of macromolecular crowding for signal transduction and metabolite channeling
4. Metabolic channeling versus free diffusion: Transition-time analysis
5. The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media
6. Diffusion measurements by photobleaching recovery methods
7. Constrained diffusion or immobile fraction on cell surfaces: A new interpretation
8. Analysis of fluorophore diffusion by continuous distributions of diffusion coefficients: Application to photobleaching measurements of simple and anomalous diffusion
9. Determinants of the translational diffusion of a small solute in cell cytoplasm
10. Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy
11. Reversible photobleaching of fluorescein conjugates in air-saturated viscous solutions: Molecular tryptophan as a triplet state quencher
12. Photochemical properties of green fluorescent protein GFP-S65T in solution and transfected CHO cells: Analysis of cytoplasmic viscosity by GFP translational and rotational diffusion
13. Diffusion in the endoplasmic reticulum of an aquaporin-2 mutant causing human nephrogenic diabetes insipidus
14. Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy
15. Fluorescence correlation spectroscopy: Diagnostics for sparse molecules
16. Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solutions and on cell surfaces
17. Fluorescence correlation spectroscopy. II. An experimental realization
18. Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microscopy
19. Diffusion in the aqueous compartment
20. A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm
21. Cytoplasmic viscosity near the cell plasma membrane: Translation of BCECF measured by total internal reflection-fluorescence photobleaching recovery
22. Probing the structure of the cytoplasm
23. Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells
24. Diffusional transport of macromolecule in developing nerve processes
25. Diffusion of fluorescence labeled macromolecules in cultured muscle cells
26. Translational diffusion of macromolecule-size solutes in cytoplasm and nucleus
27. Reduced protein diffusion rate by cytoskeleton in vegetative and polarized dictyostelium cells
28. Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy
29. Cytoarchitecture and physical properties of cytoplasm: Volume, viscosity, diffusion, intracellular surface area
30. Biological barriers to cellular delivery of lipid-based DNA carriers
31. Size-dependent DNA mobility in cytoplasm and nucleus
32. Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells
33. Aldolase exists in both the fluid and solid phases of cytoplasm
34. Protein mobility in the cytoplasm of Escherichia coli
35. 2-Deoxyglucose and cytochalasin D modulate aldolase mobility in living 3T3 cells
36. Diffusion of metabolic enzymes in mitochondria and the cytoplasm measured by fluorescence photobleaching
37. Dynamics, structure, and function are coupled in the mitochondrial matrix
38. Rapid diffusion of green fluorescent protein in the mitochondrial matrix
39. Monte-Carlo analysis of obstructed diffusion in 3 dimensions: Application to molecular diffusion in organelles
40. Diffusion of green fluorescent protein in the aqueous lumen of endoplasmic reticulum