An Isothermal Titration Calorimetry Study on the Binding of Four Volatile General Anesthetics to the Hydrophobic Core of a Four-α-Helix Bundle Protein

Biophysical Journal

Volumn 85, Issue 5, 2003, Pages 3279-3285

  Zhang, Tao ^a   Johansson, Jonas S ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROFORM; ENFLURANE; ETHER; HALOTHANE; INHALATION ANESTHETIC AGENT; ISOFLURANE; OXYGEN; PROTEIN; SEVOFLURANE;

ALPHA HELIX; ARTICLE; CALORIMETRY; DRUG MECHANISM; DRUG PROTEIN BINDING; DRUG STRUCTURE; HALOGENATION; HYDROGEN BOND; HYDROPHOBICITY; ISOTHERM; MOLECULAR DYNAMICS; TITRIMETRY;

EID: 0242290819     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(03)74746-8     Document Type: Article

References (26)

1. Bass, R. B., P. Strop, M. Barclay, and D. C. Rees. 2002. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science. 298:1582-1587.
2. + conduction and selectivity. Science. 280:69-77.
3. 2) method for characterizing anesthetic binding to proteins: analysis of isoflurane binding to albumin. Biochemistry. 31:7069-7076.
4. 19F-NMR study. Proc. Natl. Acad. Sci. USA. 90:6478-6482.
5. Dutzler, R., E. B. Campbell, and R. MacKinnon. 2003. Gating the selectivity filter in ClC chloride channels. Science. 300:108-112.
6. Eckenhoff, R. G., and H. Shuman. 1993. Halothane binding to soluble proteins determined by photoaffinity labeling. Anesthesiology. 79:96-106.
7. Eckenhoff, R. G. 1996. An inhalational anesthetic binding domain in the nicotinic acetylcholine receptor. Proc. Natl. Acad. Sci. USA. 93:2807-2810.
8. Eckenhoff, R. G., and J. S. Johansson. 1997. Molecular interactions between inhaled anesthetics and proteins. Pharmacol. Rev. 49:343-367.
9. Eger, E. I., C. Lundgren, S. L. Miller, and W. C. Stevens. 1969. Anesthetic potencies of sulfur hexafluoride, carbon tetrafluoride, chloroform and Ethrane in dogs: Correlation with the hydrate and lipid theories of anesthetic action. Anesthesiology. 30:129-135.
10. Franks, N. P., and W. R. Lieb. 1994. Molecular and cellular mechanisms of general anaesthesia. Nature. 367:607-614.
11. Ishizawa, Y., R. Pidikiti, P. A. Liebman, and R. G. Eckenhoff. 2002. G protein-coupled receptors as direct targets of inhaled anesthetics. Mol. Pharmacol, 61:945-952.
12. Johansson, J. S., R. G. Eckenhoff, and P. L. Dutton. 1995. Binding of halothane to serum albumin demonstrated using tryptophan fluorescence. Anesthesiology, 83:316-324.
13. Johansson, J. S., B. R. Gibney, F. Rabanal, K. S. Reddy, and P. L. Dutton. 1998. A designed cavity in the hydrophobic core of a four-α-helix bundle improves volatile anesthetic binding affinity. Biochemistry. 37:1421-1429.
14. Johansson, J. S., H. Zou, and J. W. Tanner. 1999. Bound volatile general anesthetics alter both local protein dynamics and global protein stability, Anesthesiology, 90:235-245.
15. Johansson, J. S., D. Scharf, L. A. Davies, K. S. Reddy, and R. G. Eckenhoff. 2000. A designed four-α-helix bundle that binds the volatile general anesthetic halothane with high affinity. Biophys. J. 78:982-993.
16. Johansson, J. S., K. Solt, and K. S. Reddy. 2003. Binding of the general anesthetics chloroform and 2,2,2-trichloroethanol to the hydrophobic core of a four-α-helix bundle protein. Photochem. Photobiol. 77:89-95.
17. Krasowski, M. D., and N. L. Harrison. 1999. General anaesthetic actions on ligand-gated ion channels. Cell. Mol. Life Sci. 55:1278-1303.
18. Leavitt, S., and E. Friere. 2001. Direct measurement of protein binding energetics by isothermal titration calorimetry. Curr. Opin. Struct. Biol. 11:560-566.
19. Manderson, G. A., and J. S. Johansson. 2002. The role of aromatic side chains in the binding of volatile general anesthetics to a four-α-helix bundle. Biochemistry. 41:4080-4087.
20. Mascia, M. P., J. R. Trudell, and R. A. Harris. 2000. Specific binding sites for alcohols and anesthetics on ligand-gated ion channels. Proc. Natl. Acad. Sci. USA. 97:9305-9310.
21. Miller, K. W. 1985. The nature of the site of general anesthesia. Int. Rev. Neurobiol. 27:1-61.
22. Smithrud, D. B., T. B. Wyman, and F. Diederich. 1991. Enthalpically driven cyclophane-arene inclusion complexation: Solvent-dependent calorimetric studies. J. Am. Chem. Soc. 113:5420-5426.
23. Toyoshima, C., M. Nakasako, H. Nomura, and H. Ogawa. 2000. Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 resolution. Nature. 405:647-655.
24. Ueda, I., and M. Yamanaka. 1997. Titration calorimetry of anesthetic-protein interaction: negative enthalpy of binding and anesthetic potency. Biophys. J. 72:1812-1817.
25. Wiseman, T., S. Williston, J. F. Brandts, and L.-N. Lin. 1989. Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal. Biochem. 179:131-137.
26. Xu, Y., T. Seto, P. Tang, and L. Firestone. 2000. NMR study of volatile anesthetic binding to nicotinic acetylcholine receptors. Biophys. J. 78:746-751.