Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor μ2 kinase

Journal of Cell Biology

Volumn 163, Issue 2, 2003, Pages 231-236

  Jackson, Antony P ^a   Flett, Alexander ^b,c   Smythe, Carl ^b,c   Hufton, Lindsay ^a   Wettey, Frank R ^a   Smythe, Elizabeth ^b,c  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Coated vesicles; Endocytosis; Phosphorylation; Regulation; Sorting

Indexed keywords

ADAPTOR PROTEIN; CLATHRIN; DOXYCYCLINE; PROTEIN KINASE; TRANSCRIPTION FACTOR AP 2;

ARTICLE; CELL GROWTH; CELL MEMBRANE PERMEABILITY; ENDOCYTOSIS; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME SUBUNIT; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING;

ADAPTOR PROTEIN COMPLEX 2; ADAPTOR PROTEIN COMPLEX MU SUBUNITS; ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE; CLATHRIN; CLATHRIN-COATED VESICLES; COATED PITS, CELL-MEMBRANE; ENDOCYTOSIS; MICE; MICE, KNOCKOUT; MODELS, BIOLOGICAL; PHOSPHORYLATION; PHOSPHOTRANSFERASES; PROTEIN BINDING; RECEPTORS, TRANSFERRIN;

GALLUS GALLUS;

EID: 0242266927     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200304079     Document Type: Article

References (31)

1. Ball, C.L., S.P. Hunt, and M.S. Robinson. 1995. Expression and localization of α-adaptin isoforms. J. Cell Sci. 108:2865-2875.
2. Campbell, C., J. Squicciarini, M. Shia, P.F. Pilch, and R.E. Fine. 1984. Identification of a protein kinase as an intrinsic component of rat liver coated vesicles. Biochemistry. 23:4420-4426.
3. Cheetham, M.E., B.H. Anderton, and A.P. Jackson. 1996. Inhibition of hsc70-catalysed clathrin uncoating by HSJ1 proteins. Biochem. J. 319:103-108.
4. Collins, B.M., A.J. McCoy, H.M. Kent, P.R. Evans, and D.J. Owen. 2002. Molecular architecture and functional model of the endocytic AP2 complex. Cell. 109:523-535.
5. Conner, S.D., and S.L. Schmid. 2002. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol. 156:921-929.
6. Conner, S.D., and S.L. Schmid. 2003a. Regulated portals of entry into the cell. Nature. 422:37-44.
7. Conner, S.D., and S.L. Schmid. 2003b. Differential requirements for AP-2 in clathrin-mediated endocytosis. J. Cell Biol. 162:773-780.
8. Cross, D.A., and C. Smythe. 1998. PD 98059 prevents establishment of the spindle assembly checkpoint and inhibits the G2-M transition in meiotic but not mitotic cell cycles in Xenopus. Exp. Cell Res. 241:12-22.
9. Gaidarov, I., and J.H. Keen. 1999. Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits. J. Cell Biol. 146:755-764.
10. Ghosh, P., and S. Kornfeld. 2003. AP-1 binding to sorting signals and release from clathrin-coated vesicles is regulated by phosphorylation. J. Cell Biol. 160: 699-708.
11. Kemp, B.E., and R.B. Pearson. 1991. Intrasteric regulation of protein kinases and phosphatases. Biochim. Biophys. Acta. 1094:67-76.
12. Kirchhausen, T. 1999. Adaptors for clathrin-mediated traffic. Annu. Rev. Cell Dev. Biol. 15:705-732.
13. Matsui, W., and T. Kirchhausen. 1990. Stabilization of clathrin coats by the core of the clathrin-associated protein complex AP-2. Biochemistry. 29:10791-10798.
14. McLauchlan, H., J. Newell, N. Morrice, A. Osborne, M. West, and E. Smythe. 1998. A novel role for rab5-GDI in ligand sequestration into dathrin-coated pits. Curr. Biol. 8:34-45.
15. Morgan, J.R., K. Prasad, W. Hao, G.J. Augustine, and E.M. Lafer. 2000. A conserved clathrin assembly motif essential for synaptic vesicle endocytosis. J. Neurosci. 20:8667-8676.
16. Ohno, H., J. Stewart, M.C. Fournier, H. Bosshart, I. Rhee, S. Miyatake, T. Saito, A. Gallusser, T. Kirchhausen, and J.S. Bonifacino. 1995. Interaction of ryrosine-based setting signals with clathrin-associated proteins. Science. 269: 1872-1875.
17. Olusanya, O., P.D. Andrews, J.R. Swedlow, and E. Smythe. 2001. Phosphorylation of threonine156 of the μ2 subunit of the AP2 complex is essential for endocytosis in vitro and in vivo. Curr. Biol. 11:896-900.
18. Owen, D.J., and P.R. Evans. 1998. A structural explanation for the recognition of tyrosine-based endocytotic signals. Science. 282:1327-1332.
19. Owen, D.J., Y. Vallis, M.E.M. Noble, J.B. Hunter, D.R. Dafforn, P.R. Evans, and H.T. McMahon. 1999. A structural explanation for the binding of multiple ligands to the α-adaptin appendage domain. Cell. 97:1-20.
20. Pauloin, A., and P. Jolles. 1984. Internal control of the coated vesicle pp50-specific kinase complex. Nature. 311:265-267.
21. Pauloin, A., and C. Thurieau. 1993. The 50 kda protein subunit of assembly polypeptide (ap) ap-2 adapter from clathrin-coated vesicles is phosphorylated on threonine-156 by ap-1 and a soluble ap50 kinase which copurifies with the assembly polypeptides. Biochem. J. 296:409-415.
22. Perham, R.N., L.C. Packman, and S.E. Radford. 1987. 2-Oxo acid dehydrogenase multi-enzyme complexes: in the beginning and halfway there. Biochem. Soc. Symp. 54:67-81.
23. Rapoport, I., M. Miyazaki, W. Boll, B. Duckworth, L.C. Cantley, S. Shoelson, and T. Kirchhausen. 1997. Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes. EMBO J. 16:2240-2250.
24. Ricotta, D., S.D. Conner, S.L. Schmid, K. von Figura, and S. Honing. 2002. Phosphorylation of the AP2 μ subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol. 156:791-795.
25. Rohde, G., D. Wenzel, and V. Haucke. 2002. A phosphatidylinositol (4,5)-bisphosphate binding site within μ2-adaptin regulates clathrin-mediated endocytosis. J. Cell Biol. 158:209-214.
26. Shih, W., A. Gallusser, and T. Kirchhausen. 1995. A clathrin-binding site in the hinge of the beta 2 chain of mammalian AP-2 complexes. J. Biol. Chem. 270: 31083-31090.
27. Smythe, E., and K. Ayscough. 2003. The Ark/Prk kinase family: regulators of endocytosis and the actin cytoskeleton. EMBO Rep. 4:246-251.
28. Smythe, E., L.L. Carter, and S.L. Schmid. 1992. Cytosol-dependent and clathtin-dependent stimulation of endocytosis in vitro by purified adapters. J. Cell Biol. 119:1163-1171.
29. Smythe, E., P.D. Smith, S.M. Jacob, J. Theobald, and S.E. Moss. 1994. Endocytosis occurs independently of annexin-VI in human a431 cells. J. Cell Biol. 124:301-306.
30. Wettey, F.R., S.F. Hawkins, A. Stewart, J.P. Luzio, J.C. Howard, and A.P. Jackson. 2002. Controlled elimination of clathrin heavy-chain expression in DT40 lymphocytes. Science. 297:1521-1525.
31. Winkler, F.K., and K.K. Stanley. 1983. Clathrin heavy-chain, light chain intetactions. EMBO J. 2:1393-1400.