Screening of two complementary collections of Saccharomyces cerevisiae to identify enzymes involved in stereo-selective reductions of specific carbonyl compounds: An alternative to protein purification

Enzyme and Microbial Technology

Volumn 33, Issue 2-3, 2003, Pages 163-172

  Katz, Michael ^a   Hahn Hägerdal, Bärbel ^a   Gorwa Grauslund, Marie F ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Deletion library; Diacetyl; Ethyl acetoacetate; NADPH; Open reading frame; Overexpression library; Reductase; Screening; Yeast

Indexed keywords

CATALYST SELECTIVITY; ENZYME KINETICS; MICROBIOLOGY; REDUCTION; STEREOCHEMISTRY;

STEREOSELECTIVITY;

ENZYMES;

2,3 BUTANEDIONE; 3 OXO ESTER ETHYL ACETOACETATE; ACETOACETIC ACID DERIVATIVE; ACETOIN DEHYDROGENASE; CARBONYL DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVITY; GENE DELETION; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PROMOTER REGION; PROTEIN PURIFICATION; REDUCTION; SACCHAROMYCES CEREVISIAE; STEREOCHEMISTRY; STRAIN DIFFERENCE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0142186831     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00086-3     Document Type: Article

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