Purification, characterization, cDNA cloning and expression of a novel ketoreductase from Zygosaccharomyces rouxii

European Journal of Biochemistry

Volumn 267, Issue 17, 2000, Pages 5493-5501

  Costello, Colleen A ^a   Payson, Robert A ^a   Menke, Michael A ^a   Larson, Jeffrey L ^a   Brown, Keith A ^a   Tanner, Joseph E ^a   Kaiser, Raymond E ^a   Hershberger, Charles L ^a   Zmijewski, Milton J ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Enzyme purification; Gene cloning; Ketoreductase; Zygosaccharomyces rouxii

Indexed keywords

ACETONE; CARBONYL REDUCTASE; COMPLEMENTARY DNA; DIKETONE; KETONE DERIVATIVE; LACTAM DERIVATIVE; LACTONE DERIVATIVE; OXIDOREDUCTASE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; ZYGOSACCHAROMYCES;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; ZYGOSACCHAROMYCES;

EID: 0033858067     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01608.x     Document Type: Article

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