Characterization of the Saccharomyces cerevisiae YMR318c (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: Relevance in aldehyde reduction

Biochemical Journal

Volumn 361, Issue 1, 2002, Pages 163-172

  Larroy, Carol ^a   Fernández, M Rosario ^a   González, Eva ^a   Parés, Xavier ^a   Biosca, Josep A ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Cinnamyl alcohol dehydrogenase; Fusel alcohols; Lignin metabolism; Veratraldehyde

Indexed keywords

ALCOHOLS; ALDEHYDES; BIODEGRADATION; BIOSYNTHESIS; CATALYST ACTIVITY; CELLS; GENES; LIGNIN; OXIDATION; REDUCTION; TOXICITY; YEAST; ZINC;

GENE PRODUCT;

ENZYMES;

ALCOHOL DEHYDROGENASE; ALCOHOL DERIVATIVE; ALDEHYDE; ALDEHYDE DERIVATIVE; ANISALDEHYDE; CINNAMALDEHYDE; GENE PRODUCT; HEXANAL; ISOVALERALDEHYDE; LIGNIN; LIGNIN PEROXIDASE; LIGNOCELLULOSE; PENTANAL; PROTEIN YMR318C; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; VERATRALDEHYDE; ZINC;

ADH6 GENE; ARTICLE; ENZYME SPECIFICITY; ENZYME SUBUNIT; GENE DISRUPTION; GENE ISOLATION; GENE OVEREXPRESSION; LETHAL GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PURIFICATION; REDUCTION; SACCHAROMYCES CEREVISIAE; YEAST;

ALCOHOL DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; ALDEHYDES; AMINO ACID SEQUENCE; BENZALDEHYDES; GENE DELETION; GENE EXPRESSION; GENES, FUNGAL; ISOELECTRIC POINT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MOLECULAR WEIGHT; OXIDATION-REDUCTION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

FUNGI; MYXOGASTRIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036182468     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3610163     Document Type: Article

References (55)

1. Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases
2. Molecular characterization of microbial alcohol dehydrcgenases
3. A super-family of medium-chain dehydrogenases/reductases (MDR). Sub-lines including zeta-crystallin, alcohol and polyol dehydrogenases, quinone oxidoreductase enoyl reductases, VAT-1 and other proteins
4. SDR and MDR: Completed genome sequences show these protein families to be large, of old origin, and of complex nature
5. Variations and constant patterns in eukaryotic MDR enzymes. Conclusions from novel structures and characterized genomes
6. Dual relationships of xylitol and alcohol dehydrogenases in families of two protein types
7. The alcohol dehydrcgenase system
8. Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene
9. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase
10. Genetics of alcohol dehydrcgenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADH II
11. The two major isozymes of yeast alcohol dehydrogenase
12. Nucleotide sequence of the yeast alcohol dehydrogenase II gene
13. Isolation and DNA sequence of ADH3, a nuclear gene encoding the mitochondrial isozyme of alcohol dehydrogenase in Saccharomyces cerevisiae
14. Complete DNA sequence of yeast chromosome II
15. Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product
16. Class III alcohol dehydrogenase from Saccharomyces cerevisiae: Structural and enzymatic features differ toward the human/mammalian forms in a manner consistent with functional needs in formaldehyde detoxication
17. A double residue substitution in the coenzyme-binding site accounts for the different kinetic properties between yeast and human formaldehyde dehydrogenases
18. Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae
19. Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase
20. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C
21. Tackling the protease problem in Saccharomyces cerevisiae
22. Improved tools for biological sequence comparison
23. A comprehensive set of sequence analysis programs for the VAX
24. Transformation of intact yeast cells treated with alkali cations
25. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
26. One-step gene disruption in yeast
27. The YDp plasmids: A uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae
28. Native gel activity staining and preparative electrophoretic method for the detection and purification of pyridine nucleotide-linked dehydrogenases
29. Disruption of seven hypothetical aryl alcohol dehydrogenase genes from Saccharomyces cerevisiae and construction of a multiple knock-out strain
30. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
31. Rapid isoelectric focusing in a vertical polyacrylamide minigel system
32. Overexpression, purification and characterization of Mycobacterium bovis BCG alcohol dehydrogenase
33. Thermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. strain M-1: Purification and characterization and gene expression in Escherichia coli
34. A novel type of pathogen defense-related cinnamyl alcohol dehydrogenase
35. Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol: NADP(+) oxidoreductase
36. A molecular model for cinnamyl alcohol dehydrogenase, a plant aromatic alcohol dehydrogenase involved in lignification
37. Brown-midrib maize (bm1)-a mutation affecting the cinnamyl alcohol dehydrogenase gene
38. Differences between alcohol dehydrogenases. Structural properties and evolutionary aspects
39. Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity
40. Catalysis by yeast alcohol dehydrogenase
41. Molecular characterisation and expression of a wound-inducible cDNA encoding a novel cinnamyl-alcohol dehydrogenase enzyme in lucerne (Medicago sativa L.)
42. A novel aromatic alcohol dehydrogenase in higher plants: Molecular cloning and expression
43. Genomic expression programs in the response of yeast cells to environmental changes
44. Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae
45. NADP-dependent alcohol dehydrogenases in bacteria and yeast: Purification and partial characterization of the enzymes from Acinetobacter calcoaceticus and Saccharomyces cerevisiae
46. Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharornyces cerevisiae
47. Aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium. Gene cloning, sequence analysis, expression, and purification of the recombinant enzyme
48. Microbial transformations of ferulic acid by Saccharomyces cerevisiae and Pseudomonas fluorescens
49. Growth of Saccharomyces cerevisiae, Rhodotorula rubra and Bullera alba in the presence of beechwood prehydrolyzate-based lignin fractions
50. Production of organoleptic compounds
51. Fusel oil
52. A 13C nuclear magnetic resonance investigation of the metabolism of leucine to isoamyl alcohol in Saccharomyces cerevisiae
53. Energy-yielding metabolism