Improving the stereoselectivity of bakers' yeast reductions by genetic engineering

Organic Letters

Volumn 1, Issue 8, 1999, Pages 1153-1155

  Rodríguez, Sonia ^a   Kayser, Margaret ^b   Stewart, Jon D ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF NEW BRUNSWICK   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

FATTY ACID SYNTHASE; ISOENZYME;

ARTICLE; CATALYSIS; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

CATALYSIS; FATTY ACID SYNTHETASE COMPLEX; GENETIC ENGINEERING; ISOENZYMES; SACCHAROMYCES CEREVISIAE;

EID: 0033592487     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol9901523     Document Type: Article

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24. 16 The biotransformation was started with 0.2 g of washed cells in 100 mL of YEP-2% carbon source along with 0.1 g of substrate. The culture was incubated in an orbital shaker at 30°C and sampled periodically for GLC analysis. After the substrate had been consumed, the product was extracted with ethyl acetate and the enantio or diastereomeric purity was established by chiral phase GLC. Further details can be found in the Supporting Information.
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