A general method allowing the design of oligonucleotide primers to amplify the variable regions from immunoglobulin cDNA

Journal of Immunological Methods

Volumn 279, Issue 1-2, 2003, Pages 251-266

  Essono, S ^a   Frobert, Y ^a   Grassi, J ^a   Creminon C ^a   Boquet, D ^a  

^a Institut National de la Recherche Agronomique   (France)

Author keywords

Mouse immunoglobulin; RT PCR; ScFv; Variable region cloning

Indexed keywords

COMPLEMENTARY DNA; IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; OLIGONUCLEOTIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE IDENTIFICATION; GERM LINE; HYBRIDIZATION; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0142124331     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00242-4     Document Type: Article

References (37)

1. Argon Y., Burrone O.R., Milstein C. Molecular characterization of a nonsecreting myeloma mutant. Eur. J. Immunol. 13:1983;301-305.
2. Boquet D., Creminon C., Clement G., Frobert Y., Nevers M.C., Essono S., Grassi J. Quantitative measurement of bitagged recombinant proteins using an immunometric assay: application to an anti-substance P recombinant antibody. Anal. Biochem. 284:2000;221-230.
3. Carroll W.L., Mendel E., Levy S. Hybridoma fusion cell lines contain an aberrant kappa transcript. Mol. Immunol. 25:1988;991-995.
4. Carter P. Improving the efficacy of antibody-based cancer therapies. Rev. Cancer Nat. 1:2001;118-129.
5. Chardes T., Villard S., Ferrieres G., Piechaczyk M., Cerutti M., Devauchelle G., Pau B. Efficient amplification and direct sequencing of mouse variable regions from any immunoglobulin gene family. FEBS Lett. 452:1999;386-394.
6. Clackson T., Hoogenboom H.R., Griffiths A.D., Winter G. Making antibody fragments using phage display libraries. Nature. 352:1991;624-628.
7. Cochet O., Martin E., Fridman W.H., Teillaud J.L. Selective PCR amplification of functional immunoglobulin light chain from hybridoma containing the aberrant MOPC 21-derived V kappa by PNA-mediated PCR clamping. Biotechniques. 26:1999;818-820.
8. Coloma M.J., Larrick J.W., Ayala M., Gavilondo-Cowley J.V. Primer design for the cloning of immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells using the PCR. Biotechniques. 11:1991;152-154.
9. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16:1988;10881-10890.
10. Cumbers S.J., Williams G.T., Davies S.L., Grenfell R.L., Takeda S., Batista F.D., Sale J.E., Neuberger M.S. Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines. Nat. Biotechnol. 11:2002;1129-1134.
11. Hafner F.T., Kautz R.A., Iverson B.L., Tim R.C., Karger B.L. Noncompetitive immunoassay of small analytes at femtomolar level by affinity probe capillary electrophoresis: direct analysis of digoxin using a uniform-labeled ScFv immunoreagent. Anal. Chem. 72:2000;5779-5786.
12. Haines B.B., Angeles C.V., Parmelee A.P., McLean P.A., Brodeur P.H. Germline diversity of the expressed BALB/c VhJ558 gene family. Mol. Immunol. 38:2001;9-18.
13. Heinrichs A., Milstein C., Gherardi E. Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR. J. Immunol. Methods. 178:1995;241-251.
14. Honegger A., Plückthun A. The influence of the buried glutamine or glutamate residue in position 6 on the structure of immunoglobulin variable domains. J. Mol. Biol. 309:2001;687-699.
15. Horton R.M., Hunt H.D., Ho S.N., Pullen J.K., Pease L.R. Engineering hybrid genes without the uses of restriction enzymes: gene splicing by overlap extension. Gene. 77:1989;61-68.
16. Jung S., Spinelli S., Schimmele B., Honegger A., Pugliese L., Cambillau C., Pluckthun A. The importance of framework residues H6, H7 and H10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody V(H) domains. J. Mol. Biol. 309:2001;701-716.
17. Kettleborough C.A., Saldanha J., Ansell K.H., Bendig M.M. Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction. Eur. J. Immunol. 23:1993;206-211.
18. Kohler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6:1976;511-519.
19. Krebber A., Bornhauser S., Burmester J., Honegger A., Willuda J., Bosshard H.R., Plückthun A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods. 201:1997;35-55.
20. Kutemeier G., Harloff C., Mocikat R. Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction. Hybridoma. 11:1992;23-32.
21. Lan F., Umeda M., Inoue K. Extended fitness of variable region primers by a novel PCR protocol. J. Immunol. Methods. 195:1996;27-32.
22. LeBoeuf R.D., Galin F.S., Hollinger S.K., Peiper S.C., Blalock J.E. Cloning and sequencing of immunoglobulin variable-region genes using degenerate oligodeoxyribonucleotides and polymerase chain reaction. Gene. 82:1989;371-377.
23. Lefranc M.P. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 29:2001;207-209.
24. Lefranc M.P., Giudicelli V., Ginestoux C., Bodmer J., Muller W., Bontrop R., Lemaître M., Malik A., Barbie V., Chaume D. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 27:1999;209-212.
25. McCafferty J., Griffiths A.D., Winter G., Chiswell D.J. Phage antibodies: filamentous phage displaying antibody variable domains. Nature. 348:1990;552-554.
26. Nakaki T., Deans R.J., Lee A.S. Enhanced transcription of the 78,000-Dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1). Mol. Cell. Biol. 9:1989;2233-2238.
27. Orlandi R., Gussow D.H., Jones P.T., Winter G. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc. Natl. Acad. Sci. U. S. A. 86:1989;3833-3837.
28. Peeters K., De Wilde C., De Jaeger G., Angenon G., Depicker A. Production of antibodies and antibody fragments in plants. Vaccine. 19:2001;2756-2761.
29. Pollock D.P., Kutzko J.P., Birck-Wilson E., Williams J.L., Echelard Y., Meade H.M. Transgenic milk as a method for the production of recombinant antibodies. J. Immunol. Methods. 231:1999;147-157.
30. Pradelles P., Grassi J., Maclouf J. Enzyme immunoassays of eicosanoids using acetylcholine esterase as label: an alternative to radioimmunoassay. Anal. Chem. 57:1985;1170-1183.
31. Remy M.H., Frobert Y., Grassi J. Characterization of monoclonal antibodies that strongly inhibit Electrophorus electricus acetylcholinesterase. Eur. J. Biochem. 231:1995;651-658.
32. Ruiz M., Giudicelli V., Ginestoux C., Stoehr P., Robinson J., Bodmer J., Marsh S.G.E., Bontrop R., Lefranc G., Chaume D., Lefranc M.P. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 28:2000;219-221.
33. Thiebe R., Schable K.F., Bensch A., Brensing-Kuppers J., Heim V., Kirschbaum T., Mitlohner H., Ohnrich M., Pourrajabi S., Roschenthaler F., Schwendinger J., Wichelhaus D., Zocher I., Zachau H.G. The variable genes and gene families of the mouse immunoglobulin kappa locus. Eur. J. Immunol. 29:1999;2072-2081.
34. Verma R., Boleti E., George A.J. Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J. Immunol. Methods. 216:1998;165-181.
35. Vidarsson G., van de Winkel J.G., van Dijk M.A. Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes. J. Immunol. Methods. 249:2001;245-252.
36. Wang Z., Raifu M., Howard M., Smith L., Hansen D., Goldsby R., Ratner D. Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3′ to 5′ exonuclease activity. J. Immunol. Methods. 233:2000;167-177.
37. Weiner L.M., Adams G.P. New approaches to antibody therapy. Oncogene. 2000;6144-6151.