Substrate turnover and inhibitor binding as selection parameters in directed evolution of blood coagulation factor Xa

Organic and Biomolecular Chemistry

Volumn 1, Issue 10, 2003, Pages 1657-1663

  Lorentsen, Rikke H ^a   Møller, Charlotte H ^a   Etzerodt, Michael ^a,b   Thøgersen, Hans C ^a,b   Holtet, Thor L ^b  

^a AARHUS UNIVERSITY   (Denmark)

^b Borean Pharma A/S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CATALYST SUPPORTS; CHEMICAL BONDS; DERIVATIVES; ENZYME IMMOBILIZATION; ENZYME INHIBITION; PROTEINS; SUBSTRATES;

BLOOD COAGULATION FACTOR; PEPTIDE; PROTEASES; SOYBEAN TRYPSIN INHIBITOR;

ENZYMES;

BOVINAE; GLYCINE MAX;

BLOOD CLOTTING FACTOR 10A; PEPTIDE LIBRARY; RECOMBINANT PROTEIN; SERINE PROTEINASE; TRYPSIN; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CATTLE; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; DRUG ANTAGONISM; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; SOYBEAN;

AMINO ACID SEQUENCE; ANIMALS; CATALYTIC DOMAIN; CATTLE; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; FACTOR XA; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SOYBEANS; SUBSTRATE SPECIFICITY; TRYPSIN; TRYPSIN INHIBITORS;

EID: 0142009629     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/b210149a     Document Type: Article

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