A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria

Genome Research

Volumn 13, Issue 9, 2003, Pages 2190-2194

  Cotta de Almeida, Vinícius ^a,b,c   Schonhoff, Susan ^d   Shibata, Tomoyuki ^a,b   Leiter, Andrew ^d   Snapper, Scott B ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c INSTITUTO OSWALDO CRUZ   (Brazil)

^d TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; PLASMID DNA;

ANALYTIC METHOD; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIAL GENETICS; CONTROLLED STUDY; DNA TEMPLATE; DNA VECTOR; ESCHERICHIA COLI; GENE CONSTRUCT; GENE LIBRARY; GENE TARGETING; GENETIC ENGINEERING; GENETIC PROCEDURES; GENETIC SCREENING; GENOME ANALYSIS; HOMOLOGOUS RECOMBINATION; IN SITU HYBRIDIZATION; KNOCKOUT MOUSE; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ANIMALS; CARRIER PROTEINS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; ESCHERICHIA COLI; GENE TARGETING; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; NOD2 SIGNALING ADAPTOR PROTEIN; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0141853943     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.1356503     Document Type: Article

References (15)

1. Angrand, P.O., Daigle, N., van der Hoeven, F., Scholer, H.R., and Stewart, A.F. 1999. Simplified generation of targeting constructs using ET recombination. Nucleic Acids Res. 27: 16.
2. Cherepanov, P.P. and Wackernagel, W. 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158: 9-14.
3. Copeland, N.G., Jenkins, N.A., and Court, D.L. 2001. Recombineering: A powerful new tool for mouse functional genomics. Nat. Rev. Genet. 2: 769-779.
4. Court, D.L., Sawitzke, J.A., and Thomason, L.C. 2002. Genetic engineering using homologous recombination. Annu. Rev. Genet. 36: 361-388.
5. Datsenko, K.A. and Wanner, B.I.. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. 97: 6640-6645.
6. Eggleston, A.K. and West, S.C. 1996. Exchanging partners: Recombination in E. coli. Trends Genet. 12: 20-26.
7. Hugot, J.P., Chamaillard, M., Zouali, H., Lesage, S., Cezard, J.P., Belaiche, J., Almer, S., Tysk, C., O'Morain, C.A., Gassull, M., et al. 2001. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 411: 599-603.
8. Lee, E.C., Yu, D., Martinez de Velasco, J., Tessarollo, L., Swing, D.A., Court, D.L., Jenkins, N.A., and Copeland, N.G. 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73: 56-65.
9. Liu, P., Jenkins, N.A., and Copeland, N.G. 2003. A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res. 13: 476-484.
10. Murphy, K.C. 1998. Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol. 180: 2063-2071.
11. Muyrers, J.P., Zhang, Y., Testa, G., and Stewart, A.F. 1999. Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res. 27: 1555-1557.
12. Ogura, Y., Bonen, D.K., Inohara, N., Nicolae, D.L., Chen, F.F., Ramos, R., Britton, H., Moran, T., Karaliuskas, R., Duerr, R.H., et al. 2001. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 411: 603-606.
13. Yu, D., Ellis, H.M., Lee, E.C., Jenkins, N.A., Copeland, N.G., and Court, D.L. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. 97: 5978-5983.
14. Zhang, P., Li, M.Z., and Elledge, S.J. 2002. Towards genetic genome projects: Genomic library screening and gene-targeting vector construction in a single step. Nat. Genet. 30: 31-39.
15. Zhang, Y., Buchholz, F., Muyrers, J.P., and Stewart, A.F. 1998. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20: 123-128.