Towards genetic genome projects: Genomic library screening and gene-targeting vector construction in a single step

Nature Genetics

Volumn 30, Issue 1, 2002, Pages 31-39

  Zhang, Pumin ^a   Li, Mamei Z ^a,b   Elledge, Stephen J ^a,b  

^a Department of Molecular Physiology and Biophysics ^*  (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; DNA; DNA FRAGMENT; PHOSPHOGLYCERATE KINASE; SINGLE STRANDED DNA;

ARTICLE; BACTERIOPHAGE; DNA VECTOR; ESCHERICHIA COLI; GENE DISRUPTION; GENE LIBRARY; GENE TARGETING; GENETIC RECOMBINATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ANIMALS; BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DRUG RESISTANCE; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; FEASIBILITY STUDIES; GENE EXPRESSION REGULATION, BACTERIAL; GENE LIBRARY; GENE TARGETING; GENES; GENETIC VECTORS; GENOME; GENOME, BACTERIAL; GENOMICS; ISOPROPYL THIOGALACTOSIDE; MALE; MICE; NEOPLASM PROTEINS; PLASMIDS; RECOMBINATION, GENETIC; SELECTION (GENETICS); SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECTINOMYCIN; VIRAL PROTEINS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036340432     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng797     Document Type: Article

References (29)

1. Gene targeting vectors for mammalian cells
2. The initiation and control of homologous recombination in Escherichia coli
3. Isolation of exonuclease VIII: The enzyme associated with sbcA indirect suppressor
4. The segregation of the SbcA and Rac phenotypes in an Escherichia coli recB mutant
5. Recombination deficient mutants of E. Coli and other bacteria
6. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli
7. Use of bacteriophage γ recombination functions to promote gene replacement in Escherichia coli
8. Roles of RuvC and RecG in phage γ red-mediated recombination
9. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants
10. γ YES a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations
11. Position and density effects on repression by stationary and mobile DNA-binding proteins
12. A complex control circuit. Regulation of immunity in temperate bacteriophages
13. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes
14. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1
15. The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome
16. A genetic link between morphogenesis and cell division during formation of the ventral furrow in Drosophila
17. Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis
18. Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase
19. Functional characterization of the S. Cerevisiae genome by gene deletion and parallel analysis
20. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells
21. A new logic for DNA engineering using recombination in Escherichia coli
22. An efficient recombination system for chromosome engineering in Escherichia coli
23. Simplified generation of targeting constructs using ET recombination
24. Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse
25. Construction of gene targeting vectors from lambda KOS genomic libraries
26. DNA cloning by homologous recombination in Escherichia coli
27. Novel bacteriophage γ cloning vector
28. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: A general strategy for targeting mutations to non-selectable genes
29. Positive selection for loss of tetracycline resistance