A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity

Analytical Biochemistry

Volumn 322, Issue 1, 2003, Pages 33-39

  Parniak, Michael A ^a   Min, Kyung Lyum ^b   Budihas, Scott R ^c   Le Grice, Stuart F J ^c   Beutler, John A ^c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b MCGILL UNIVERSITY   (Canada)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Chemical library screening; Fluorescence resonance energy transfer; Reverse transcriptase; Ribonuclease H

Indexed keywords

ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; RNA; ROBOTICS; VIRUSES;

CHEMICAL LIBRARIES; COEFFICIENTS OF VARIATIONS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENCE RESONANCE ENERGY TRANSFER ASSAYS; HIGH THROUGHPUT SCREENING; HUMAN IMMUNODEFICIENCY VIRUS-1; REVERSE TRANSCRIPTASES; RIBONUCLEASE H;

DISEASES;

COMPLEMENTARY DNA; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; CORRELATION COEFFICIENT; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; FLUORESCENCE; HUMAN IMMUNODEFICIENCY VIRUS 1; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; RNA STRUCTURE; ROOM TEMPERATURE; SCREENING TEST;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0141758137     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.06.001     Document Type: Article

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