Protection conferred by Bcl-2 expression involves reduced oxidative stress and increased glutathione production during hypothermia-induced apoptosis in AK-5 tumor cells

Free Radical Biology and Medicine

Volumn 35, Issue 8, 2003, Pages 949-957

  Khar, Ashok ^a   Pardhasaradhi, B V V ^a   Ali, A Mubarak ^a   Kumari, A Leela ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

AK 5 cells; Apoptosis; Bcl 2 expression; Free radicals; Glutathione; Hypothermia; Superoxide

Indexed keywords

GLUTATHIONE; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; SUPEROXIDE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL GROWTH; CONTROLLED STUDY; GENETIC TRANSFECTION; GLUTATHIONE METABOLISM; HYPOTHERMIA; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

MAMMALIA;

EID: 0141749468     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(03)00469-6     Document Type: Article

References (34)

1. Overgaard J. The current and potential role of hyperthermia in radiotherapy. Int. J. Radiat. Oncol. Biol. Phys. 16:1989;535-549.
2. Slikker W. III, Desai V.G., Duhart H., Feuers R., Imam S.Z. Hypothermia enhances bcl-2 expression and protects against oxidative stress-induced cell death in Chinese hamster ovary cells. Free Radic. Biol. Med. 31:2001;405-411.
3. Bossenmeyer-Pourie C., Kozeil V., Daval J.L. Effects of hypothermia on hypoxia-induced apoptosis in cultured neurons from developing rat forebrain comparison with preconditioning . Pediatr. Res. 47:2000;385-391.
4. Robertson J.D., Datta K., Kehrer J.P. Bcl-xL overexpression restricts heat-induced apoptosis and influences hsp70, bcl-2, and Bax protein levels in FL5.12 cells. Biochem. Biophys. Res. Commun. 241:1997;164-168.
5. Voehringer D.W. BCL-2 and glutathione alterations in cellular redox state that regulate apoptosis sensitivity . Free Radic. Biol. Med. 27:1999;945-950.
6. Khar A., Ali M.A., Pardhasaradhi B.V.V., Varalakshmi C., Anjum R., Kumari A.L. Induction of stress response renders human tumor cell lines resistant to curcumin mediated apoptosis Role of reactive oxygen intermediates . Cell Stress Chaperones. 6:2001;368-376.
7. Hockenbery D.M., Oltavi Z.N., Yin X.M., Milliman C.L., Korsemeyer S.J. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 75:1993;241-251.
8. Voehringer D.W., McConkey D.J., McDonnel T.J., Brisbay S., Meyn R.E. Bcl-2 expression causes redistribution of glutathione to the nucleus. Proc. Natl. Acad. Sci. USA. 95:1998;2956-2960.
9. Khar A. Development and characterization of a rat histiocyte/macrophage tumor line. J. Natl. Cancer Inst. 76:1986;871-878.
10. Khar A., Ali A.M. Adaptation of rat histiocytoma cells AK-5, to growth in culture. In Vitro Cell. Dev. Biol. 26:1990;1024-1025.
11. Bhaumik S., Anjum R., Rangaraj N., Paradhasaradhi B.V.V., Khar A. Curcumin mediated apoptosis in AK-5 cells involves the production of Reactive Oxygen Intermediates. FEBS Lett. 456:1999;311-314.
12. Sreedhar A.S., Paradhasaradhi B.V.V., Begum Z., Khar A., Srinivas U.K. Lack of heat shock response triggers programmed cell death in a rat histiocytic cell line. FEBS Lett. 456:1999;339-342.
13. Anjum R., Joshi P., Khar A. Induction of apoptosis in AK-5 tumor cells by a serum factor from tumor rejecting animals cytochrome c release independent of Bcl-2 and caspases . Cell Death Differ. 8:2001;1038-1046.
14. Gong J., Tragames F., Darzynkiewicz Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal. Biochem. 218:1994;314-319.
15. Tang D.G., Li L., Zhu Z., Joshi B. Apoptosis in the absence of cytochrome c accumulation in the cytosol. Biochem. Biophys. Res. Commun. 242:1998;380-384.
16. Pick E. Microassays for superoxide and hydrogen peroxide production and nitroblue tetrazolium reduction using an enzyme immunoassay microplate reader. Methods Enzymol. 132:1986;407-421.
17. 2 in cultured endothelial cells. Arch. Biochem. Biophys. 302:1993;348-352.
18. Hissin P.J., Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem. 74:1976;214-226.
19. Aruoma O.I. Characterization of drugs as antioxidant prophylactics. Free Radic. Biol. Med. 20:1996;675-705.
20. Lord-Fontaine S., Averill-Bates D.A. Heat shock inactivates cellular antioxidant defenses against hydrogen peroxide protection by glucose . Free Radic. Biol. Med. 32:2002;752-765.
21. Michiels C., Raes M., Toussaint O., Remacle J. Importance of Se-glutathione peroxidase.catalase and Cu/Zn-SOD for cell survival against oxidative stress. Free Radic. Biol. Med. 17:1994;235-248.
22. Lyras L., Cairns N.J., Jenner A., Jenner P., Halliwell B. An assessment of oxidative damage to proteins, lipids, and DNA in brain from patients with Alzheimer's disease. J. Neurochem. 68:1997;2061-2069.
23. Wyllie A.H., Kerr J.F.R., Currie A.R. Cell death the significance of apoptosis . Int. Rev. Cytol. 68:1980;251-306.
24. Reed J.C. Regulation of apoptosis by bcl-2 family proteins and its role in cancer and chemoresistance. Curr. Opin. Oncol. 7:1995;541-546.
25. Merry D.E., Korsmeyer S.J. Bcl-2 gene family in the nervous system. Annu. Rev. Neurosci. 20:1997;245-267.
26. Lee M.H., Hyun D.-H., Marshall K.-A., Ellerby L.M., Bredesen D.E., Jenner P., Halliwell B. Effect of overexpression of BCL-2 on cellular oxidative damage, nitric oxide production, antioxidant defenses, and the proteasome. Free Radic. Biol. Med. 31:2001;1550-1559.
27. Veis D.J., Sorenson C.M., Shutter J.R., Korsmeyer S.J. Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Cell. 75:1993;229-240.
28. Sarafian T.A., Bredesen D.E. Is apoptosis mediated by reactive oxygen species? Free Radic. Res. 21:1994;1-8.
29. Yang J., Liu X., Bhalla K., Kim C.N., Ibrado A.M., Cai J., Peng T.I., Jones D.P., Wang X. Prevention of apoptosis by Bcl-2 release of cytochrome c from mitochondria blocked . Science. 275:1997;1129-1132.
30. Schlessinger P.H., Gross A., Yin X.M., Yamamoto K., Saito M., Waksman G., Korsmeyer S.J. Comparison of the ion channel characteristics of proapoptotic BAX and antiapoptotic BCl-2. Proc. Natl. Acad. Sci. USA. 94:1997;11357-11362.
31. Susin S.A., Zamzami N., Kroemer G. Mitochondria as regulators of apoptosis doubt no more . Biochim. Biophys. Acta. 1366:1998;151-165.
32. Bellomo G., Variretti M., Stivala L., Miraballi F., Richelmi P., Orrenius S. Demonstration of nuclear compartmentalization of glutathione in hepatocytes. Proc. Natl. Acad. Sci. USA. 89:1992;4412-4416.
33. Buchan A. Advances in cerebral ischemia experimental approaches . Neurol. Clin. 10:1992;49-61.
34. Yoshioka T., Shires G.T., Fantini G.A. Hypothermia relieves oxidative stress in reperfused skeletal muscle after partial ischemia. J. Surg. Res. 53:1992;408-416.