Sequence-dependent pausing of single lambda exonuclease molecules

Science

Volumn 301, Issue 5641, 2003, Pages 1914-1918

  Perkins, Thomas T ^a,c   Dalal, Ravindra V ^a   Mitsis, Paul G ^b,d   Block, Steven M ^a  

^a STANFORD UNIVERSITY   (United States)

^b Praelux Inc   (United States)

^c UNIVERSITY OF COLORADO   (United States)

^d Amersham Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; ELECTROPHORESIS; PROBABILITY;

LAMBDA EXONUCLEASE;

DNA;

ADENOSINE TRIPHOSPHATE; DOUBLE STRANDED DNA; EXONUCLEASE; LAMDA EXONUCLEASE; NUCLEOTIDE; UNCLASSIFIED DRUG;

DNA;

ARTICLE; DNA DEGRADATION; DNA FLANKING REGION; DNA RECOMBINATION; DNA SEQUENCE; DNA STRAND; ENZYME INHIBITION; GEL ELECTROPHORESIS; MOLECULE; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

BACTERIOPHAGE LAMBDA; BASE PAIRING; BASE SEQUENCE; BINDING SITES; CONSENSUS SEQUENCE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXODEOXYRIBONUCLEASES; HYDROGEN BONDING; KINETICS; MODELS, CHEMICAL; OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROBABILITY; STOCHASTIC PROCESSES; TIME FACTORS; VIRAL PROTEINS;

EID: 0141534308     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1088047     Document Type: Article

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29. We thank K. Neuman and R. Knight for helpful discussions; E. Abbondanzieri for discussions and supplying his pause-finding algorithm; J. Einerson for extensive help with the assays; A. Engh, K. Herbert, and N. Padte for advice and help with sequencing gels; and U. Bali for the generous gift of lambda exonuclease. T.T.P. received support from a Burroughs Wellcome Fund Career Award in the Biomedical Sciences, a Princeton University Materials Institute Fellowship, and NIST. This work was supported by grants from the NIGMS GM 57035 (S.M.B.) and NHGRI HG 011821-01 (P.G.M.).