Novel promoter/transactivator configurations for macrolide- and streptogramin-responsive transgene expression in mammalian cells

Journal of Gene Medicine

Volumn 4, Issue 6, 2002, Pages 676-686

  Weber, Wilfried ^a   Kramer, Beat P ^a   Fux, Cornelia ^a   Keller, Bettina ^a   Fussenegger, Martin ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

E.REX; Erythromycin; Gene regulation system; Gene therapy; Pip system; Pristinamycin; Tissue engineering

Indexed keywords

ERYTHROMYCIN; MACROLIDE; NEOMYCIN; PRISTINAMYCIN; STREPTOGRAMIN; TETRACYCLINE; TRANSACTIVATOR PROTEIN; PRIMER DNA; STREPTOGRAMIN DERIVATIVE; TETRACYCLINE DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL LINE; CELL SPECIFICITY; CELL TYPE; GENE CONTROL; GENE EXPRESSION; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; OPERATOR GENE; PRIORITY JOURNAL; PROMOTER REGION; TRANSACTIVATION; TRANSGENE; ANIMAL; CELL STRAIN K 562; CHO CELL; HAMSTER; METABOLISM; NUCLEOTIDE SEQUENCE;

MAMMALIA;

ANIMALS; BASE SEQUENCE; CHO CELLS; CRICETINAE; DNA PRIMERS; HUMANS; K562 CELLS; MACROLIDES; PROMOTER REGIONS (GENETICS); STREPTOGRAMINS; TETRACYCLINES; TRANS-ACTIVATORS; TRANSGENES;

EID: 0042964105     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jgm.314     Document Type: Article

References (30)

1. Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000; 24: 372-376.
2. Fussenegger M, Schlatter S, Dätwyler D, Mazur X, Bailey JE. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol 1998; 16: 468-472.
3. Umaña P, Jean-Mairet J, Moudry R, Amstutz H, Bailey JE. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat Biotechnol 1999; 17: 176-180.
4. Aubel D, Morris R, Lennon B, et al. Design of a novel mammalian screening system for the detection of bioavailable, non-cytotoxic streptogramin antibiotics. J Antibiot 2001; 54: 44-55.
5. Kip1 sense and antisense enables positive and negative growth control of Chinese hamster ovary cells. Nucleic Acids Res 2001; 29: e19.
6. Rivera VM, Ye X, Courage NL, et al. Long-term regulated expression of growth hormone in mice after intramuscular gene transfer. Proc Natl Acad Sci U S A 1999; 96: 8657-8662.
7. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 1991; 89: 5547-5551.
8. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science 1995; 268: 1766-1769.
9. Fussenegger M, Morris RP, Fux C, et al. Streptogramin-based gene regulation systems for mammalian cells. Nat Biotechnol 2000; 18: 1203-1208.
10. Fussenegger M. The impact of mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies. Biotechnol Prog 2001; 17: 1-51.
11. Weber W, Fux C, Daoud-El Baba M, et al. Macrolide-adjustable transgene control technology in mammalian cells and mice. Nat Biotechnol 2000; in press.
12. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the transactivator of Herpes simplex virus immediate early gene expression. Genes Dev 1998; 2: 718-729.
13. Corces V, Pellicer A. Identification of sequences involved in the transcriptional control of a Drosophila heat-shock gene. J Biol Chem 1984; 259: 14 812-14 817.
14. Moser S, Schlatter S, Fux C, Rimann M, Bailey JE, Fussenegger M. An update of pTRIDENT multicistronic expression vectors: pTRIDENTs containing novel streptogramin-responsive promoters. Biotechnol Prog 2000; 16: 724-735.
15. Moser S, Rimann M, Fux C, Schlatter S, Bailey JE, Fussenegger M. Dual-regulated expression technology: a new era in the adjustment of heterologous gene expression in mammalian cells. J Gene Med 2001; 3: 529-549.
16. Freundlieb S, Schirra-Müller C, Bujard H. A tetracycline-controlled activation/repression system with increased potential for gene transfer into mammalian cells. J Gene Med 1999; 1: 4-12.
17. Senner V, Sotoodeh A, Paulus W. Regulated gene expression in glioma cells: a comparison of three inducible systems. Neurochem Res 2001; 26: 521-524.
18. Fussenegger M. Dual-regulated gene expression in mammalian cells, a novel approach to gene therapy. Gene Ther Reg 2001; 1 233-264.
19. Hoffmann A, Villalba M, Journot L, Spengler DA. A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cells. Nucleic Acids Res 1997; 25: 1078-1079.
20. Akagi K, Kanai M, Saya H, Kozu T, Berns A. A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain. Nucleic Acids Res 2001; 29: e23.
21. Baron U, Gossen M, Bujard H. Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res 1997; 25: 2723-2729.
22. Berger J, Hauber J, Hauber R, Geiger R, Cullen BR. Secreted alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 1998; 66: 1-10.
23. Fussenegger M, Moser S, Mazur X, Bailey JE. Autoregulated multicistronic expression vectors provide one-step cloning of regulated product gene expression in mammalian cells. Biotechnol Prog 1997; 13: 733-740.
24. Boshart M, Weber F, Jahn G, Dorsch-Häsler K, Fleckstein B, Schaffner W. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 1985; 41: 521-530.
25. Urlinger S, Helbl V, Guthmann J, Pook E, Grimm S, Hillen W. The p65 domain from NF-kB is an efficient human activator in the tetracycline-regulatable gene expression system. Gene 2000; 247 103-110.
26. Nourbakbsh M, Hauser H. The transcriptional silencer protein NRF: a repressor of NF-kappa B enhancers. Immunobiology 1997; 198: 65-72.
27. Parkhill J, Brown NL. Site-specific insertion and deletion mutants in the mer promoter-operator region of Tn501; the nineteen base-pair spacer is essential for normal induction of the promoter by MerR. Nucleic Acids Res 1990; 18: 5157-5162.
28. Robinett CC, Dunaway M. Modelling transcriptional regulation using microinjection into Xenopus Oocytes. Methods 1999; 17: 151-160.
29. Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endo 1997; 11: 1994-2003.
30. Youderian P, Bouvier S, Susskind MM. Sequence determinants of promoter activity. Cell 1982; 30: 843-853.