Diagnostic and prognostic value of colony formation of hematopoietic progenitor cells in myeloid malignancies

Wiener Klinische Wochenschrift

Volumn 115, Issue 13-14, 2003, Pages 537-546

  Öhler, Leopold ^a,d   Geissler, Klaus ^b   Hinterberger, Wolfgang ^c  

^a UNIVERSITY OF VIENNA   (Austria)

^b GENERAL HOSPITAL LINZ   (Austria)

^c DANUBE HOSPITAL   (Austria)

^d MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Colony assays; Diagnostic and prognostic significance; Hematopoietic progenitor cells; Myeloid malignancies

Indexed keywords

ACUTE GRANULOCYTIC LEUKEMIA; APLASTIC ANEMIA; CELL DIFFERENTIATION; CHRONIC MYELOMONOCYTIC LEUKEMIA; DIAGNOSTIC VALUE; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; MYELODYSPLASTIC SYNDROME; MYELOFIBROSIS; MYELOPROLIFERATIVE DISORDER; POLYCYTHEMIA VERA; PROGNOSIS; REVIEW; STEM CELL; THROMBOTIC THROMBOCYTOPENIC PURPURA;

EID: 0042508734     PISSN: 00435325     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03041036     Document Type: Review

References (124)

1. Bradley TR, Metcalf D (1966) The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci 44: 287-299
2. Pluznik DH, Sachs L (1966) The induction of clones of normal mast cells by a substance from conditioned medium. Exp Cell Res 1966 43: 553-563
3. Wu AM, Till JE, Siminovitch L, McCulloch EA (1966) A cytological study of the capacity for differentiation of normal hematopoietic colony-forming cells. J Cell Physiol 69: 177-184
4. Ichikawa Y, Pluznik DH, Sachs L (1966) In vitro control of the development of macrophage and granulocyte colonies. Proc Natl Acad Sci USA 56: 488-495
5. Chervenick PA, Boggs DR (1971) In vitro growth of granulocytic and mononuclear cell colonies from blood of normal individuals. Blood 37: 131-135
6. Wu AM, Siminovitch L, Till JE, McCulloch EA (1968) Evidence for a relationship between mouse hemopoietic stem cells and cells forming colonies in culture. Proc Natl Acad Sci USA 59: 1209-1215
7. Paran M, Sachs L (1969) The single cell origin of normal granulocyte colonies in vitro. J Cell Physiol 73: 91-92
8. Emerson SG, Thomas S, Ferrara JL, Greenstein JL (1989) Developmental regulation of erythropoiesis by hematopoietic growth factors: analysis on populations of BFU-E from bone marrow, peripheral blood, and fetal liver. Blood 74: 49-55
9. Metcalf D (1968) Potentiation of bone marrow colony growth in vitro by the addition of lymphoid or bone marrow cells. J Cell Physiol 72: 9-19
10. Foster R Jr, Metcalf D, Robinson WA, Bradley TR (1968) Bone marrow colony stimulating activity in human sera. Results of two independent surveys in Buffalo and Melbourne. Br J Haematol 15: 147-159
11. Robinson WA, Stanley ER, Metcalf D (1969) Stimulation of bone marrow colony growth in vitro by human urine. Blood 33: 396-399
12. Foster R Jr, Metcalf D, Kirchmyer R (1968) Induction of bone marrow colony-stimulating activity by a filterable agent in leukemic and normal mouse serum. J Exp Med 127: 853-866
13. Sheridan JW, Metcalf D (1973) A low molecular weight factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro. J Cell Physiol 81: 11-23
14. Metcalf D (1990) The colony stimulating factors. Cancer 65: 2185-2195
15. Welte K, Platzer E, Lu L, Gabrilove JL, Levi E, Mertelsmann R, Moore MA (1985) Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor. Proc Natl Acad Sci USA 82: 1526-1530
16. Fung MC, Hapel AJ, Ymer S, Cohen DR, Johnson RM, Campbell HD, Young IG (1984) Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237
17. Souza LM, Boone TC, Gabrilove J, Lai PH, Zsebo KM, Murdock DC, Chazin VR, Bruszewski J, Lu H, Chen KK, et al (1986) Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells. Science 232: 61-65
18. Wong GG, Witek JS, Temple PA, Wilkens KM, Leary AC, Luxenberg DP, Jones SS, Brown EL, Kay RM, Orr EC, et al (1985) Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228: 810-815
19. Bradley TR, Robinson W, Metcalf D (1967) Colony production in vitro by normal polycythaemic and anaemic bone marrow. Nature 214: 511
20. Stephenson JR, Axelrad AA, McLeod DL, Shreeve MM (1971) Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci USA 68: 1542-1546
21. Reissmann KR, Samorapoompichit S (1970) Effect of erythropoietin on proliferation of erythroid stem cells in the absence of transplantable colony-forming units. Blood 36: 287-296
22. Gregory CJ, McCulloch EA, Till JE (1973) Erythropoietic progenitors capable of colony formation in culture: state of differentiation. J Cell Physiol 81: 411-420
23. Geissler K, Stockenhuber F, Kabrna E, Hinterberger W, Balcke P, Lechner K (1989) Recombinant human erythropoietin and hematopoietic progenitor cells in vivo. Blood 73: 2229-22323
24. Fauser AA, Messner HA (1979) Identification of megakaryocytes, macrophages, and eosinophils in colonies of human bone marrow containing neutrophilic granulocytes and erythroblasts. Blood 53: 1023-1027
25. Worton RG, McCulloch EA, Till JE (1969) Physical separation of hemopoietic stem cells differing in their capacity for self-renewal. J Exp Med 130: 91-103
26. Gregory CJ, McCulloch EA, Till JE (1973) Erythropoietic progenitors capable of colony formation in culture: state of differentiation. J Cell Physiol 81: 411-420
27. Wu AM, Till JE, Siminovitch L, McCulloch EA (1968) Cytological evidence for a relationship between normal hemotopoietic colony-forming cells and cells of the lymphoid system. J Exp Med 127: 455-464
28. Korn AP, Henkelman RM, Ottensmeyer FP, Till JE (1973) Investigations of a stochastic model of haemopoiesis. Exp Hematol 1: 362-375
29. Breems DA, Blokland EA, Neben S, Ploemacher RE (1994) Frequency analysis of human primitive haematopoietic stem cell subsets using a cobblestone area forming cell assay. Leukemia 8: 1095-1104
30. Theilgaard-Monch K, Raaschou-Jensen K, Heilmann C, Andersen H, Bock J, Russel CA, Vindelov L, Jacobsen N, Dickmeiss E (1999) A comparative study of CD34+ cells, CD34+ subsets, colony forming cells and cobblestone area forming cells in cord blood and bone marrow allografts. Eur J Haematol 62: 174-183
31. Zhang XB, Li K, Fok TF, Li CK, James AE, Lam AC, Lee SM, Yuen PM (2002) Cobblestone area-forming cells, long-term culture-initiating cells and NOD/SCID repopulating cells in human neonatal blood: a comparison with umbilical cord blood. Bone Marrow Transplant 30: 557-564
32. Verfaillie CM, Miller JS (1995) A novel single-cell proliferation assay shows that long-term culture-initiating cell (LTC-IC) maintenance over time results from the extensive proliferation of a small fraction of LTC-IC. Blood 86: 2137-2145
33. Namikawa R, Weilbaecher KN, Kaneshima H, Yee EJ, McCune JM (1990) Long-term human hematopoiesis in the SCID-hu mouse. J Exp Med 172: 1055-1063
34. Lapidot T, Pflumio F, Doedens M, Murdoch B, Williams DE, Dick JE (1992) Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice. Science 255: 1137-1141
35. Broxmeyer HE, Lu L, Platzer E, Feit C, Juliano L, Rubin BY (1983) Comparative analysis of the influences of human gamma, alpha, and beta interferons on human multipotential (CFU-GEMM), erythroid (BFU-E), and granulocyte-macrophage (CFU-GM) progenitor cells. J Immunol 131: 1300
36. Zoumbos NC, Djeu JY, Young NS (1984) Interferon is the suppressor of hematopoiesis generated by stimulated lymphocytes. J Immunol 133: 769
37. Schooley JC, Kullgren B, Allison AC (1987) Inhibition by interleukin-1 of the action of erythropoietin on erythroid precursors and ist possible role in the pathogenesis of hypoplastic anemias. Br J Haematol 67: 11
38. Tracy KJ, Wie H, Manoque KR, Fong J, Hesse DG, Nguyen HT, Kuo GC, Beutler B, Cotran RS, Cerami A, Lowry SF (1988) Cachectin/tumor necrosis factor induces cachexia, anemia, and inflammation. J Exp Med 167: 1211
39. Johnson CS, Cook CA, Furmanski P (1990) In vivo suppression of erythropoiesis by tumor necrosis factor-alpha (TNF-alpha): reversal with exogenous erythropoietin (EPO). Exp Hematol 18: 109-113
40. Khoury E, Andre C, Pontvert-Delucq S, Drenou B, Baillou C, Guigon M, Najman A, Lemoine FM (1994) Tumor necrosis factor alpha (TNF alpha) downregulates c-kit proto-oncogene product expression in normal and acute myeloid leukemia CD34+ cells via p55 TNF alpha receptors. Blood 84: 2506-2514
41. Wang CQ, Udupa KB, Lipschitz DA (1995) Interferon-gamma exerts ist negative regulatory effect primarily on the earliest stages of murine erythroid progenitor cell development. J Cell Physiol 162: 134
42. Oehler L, Födinger M, Köller M, Kollars M, Reiter E, Bohle B, Skoupy S, Fritsch G, Lechner K, Geissler K (1997) Interleukin-10 inhibits spontaneous CFU-GM growth from human peripheral blood mononuclear cells by suppression of endogenous GM-CSF release. Blood 89: 1147-1153
43. Oehler L, Kollars M, Bohle B, Berer A, Reiter E, Lechner K, Geissler K (1999) IL-10 inhibits BFU-E growth by suppression of endogenous GM-CSF production from T cells. Exp Hematol 27: 217-223
44. Broxmeyer HE, Kim CH (1999) Regulation of hematopoiesis in a sea of chemokine family members with a plethora of redundant activities. Exp Hematol 27: 1113-1123
45. Drachman JG (2000) Role of thrombopoietin in hematopoietic stem cell and progenitor regulation. Curr Opin Hematol 7: 183-190
46. Zhu J, Emerson SG (2002) Hematopoietic cytokines, transcription factors and lineage commitment. Oncogene 21: 3295-3313
47. Robinson WA, Bradley TR, Metcalf D (1967) Effect of whole body irradiation on colony production by bone marrow cells in vitro. Proc Soc Exp Biol Med 25: 388-391
48. Ball JK, Hoshino S, McCarter JA (1973) Depressive effect of 7,12-dimethylbenz(alpha)anthracene and ionizing irradiation on bone marrow colony-forming cells. J Natl Cancer Inst 51: 1491-1495
49. Bruce WR, Meeker BE, Powers WE, Valeriote FA (1969) Comparison of the dose- and timesurvival curves for normal hematopoietic and lymphoma colony-forming cells exposed to vinblastine, vincristine, arabinosylcytosine, and amethopterin. J Natl Cancer Inst 42: 1015-1025
50. Blackett NM, Millard RE (1973) Differential effect of Myleran on two normal haemopoietic progenitor cell populations. Nature 244: 300-301
51. Ratzan RJ, Moore MA, Yunis AA (1974) Effect of chloramphenicol and thiamphenicol on the in vitro colony-forming cell. Blood 43: 363-369
52. Horoszewicz JS, Byrd DM, Sokal JE, Carter WA (1974) The colony-forming cell in the normal and leukemia human host: responses to streptovaricin and rifamycin SV. J Natl Cancer Inst 52: 649-652
53. Horoszewicz JS, Carter WA (1974) Responses of the murine myeloid colony-forming cell to ansamycin antibiotics. Antimicrob Agents Chemother 5: 196-198
54. Razek A, Valeriote F, Vietti T (1974) Survival of hematopoietic and leukemic colony-forming cells in vivo after administration of mitomycin C or porfiromycin. J Natl Cancer Inst 51: 1845-1848
55. Rosenblum ML, Knebel KD, Wheeler KT, Barker M, Wilson CB (1975) Development of an in vitro colony formation assay for the evaluation of in vivo chemotherapy of a rat brain tumor. In Vitro 11: 264-273
56. Briganti G, Galloni L, Levi G, Spalletta V, Mauro F (1975) Effects of bleomycin on mouse bone-marrow stem cells. J Natl Cancer Inst 55: 53-57
57. Yunis AA, Gross MA (1975) Drug-induced inhibition of myeloid colony growth: protective effect of colony-stimulating factor. J Lab Clin Med 86: 499-504
58. Geissler K, Tricot G, Leemhuis T, Walker E, Broxmeyer HE (1989) Differentiation-inducing effect of recombinant human tumor necrosis factor alpha and gamma-interferon in vitro on blast cells from patients with acute myeloid leukemia and myeloid blast crisis of chronic myeloid leukemia. Cancer Res 49: 3057-3062
59. Geissler K, Öhler L, Födinger M, Virgolini I, Leimer M, Kabrna E, Kollars M, Skoupy S, Bohle B, Rogy, M, Lechner K (1996) Interleukin-10 inhibits growth and granulocyte/macrophage colony-stimulating factor production in chronic myelomonocytic leukemia cells. J Exp Med 184: 1-8
60. Geissler K, Ohler L, Fodinger M, Kabrna E, Kollars M, Skoupy S, Lechner K (1998) Interleukin-10 inhibits erythropoietin-independent growth of erythroid bursts in patients with polycythemia vera. Blood 92: 1967-1972
61. The Italian Cooperative Study Group on Chronic Myeloid Leukemia (1994) Interferon alfa-2a as compared with conventional chemotherapy for the treatment of chronic myeloid leukemia. N Engl J Med 330: 820-825
62. Tura S, Baccarani M (1995) Alpha-interferon in the treatment of chronic myeloid leukemia. The Italian Cooperative Study Group on Chronic Myeloid Leukemia. Blood 85: 2999-3002
63. Socinski MA, Cannistra SA, Elias A, Antman KH, Schnipper L, Griffin JD (1988) Granulocyte-macrophage colony stimulating factor expands the circulating haemopoietic progenitor cell compartment in man. Lancet 28: 1194-1198
64. Emminger W, Emminger-Schmidmeier W, Hocker P, Hinterberger W, Gadner H (1989) Myeloid progenitor cells (CFU-GM) predict engraftment kinetics in autologous transplantation in children. Bone Marrow Transplant 4: 415-420
65. Öhler L, Scholten C, Reiter E, Tiefengraber E, Jäger U, Lechner K, Höcker P, Geissler K (1993) Mobilisierung zirkulierender hämatopoetischer Stammzellen durch G-CSF nach Chemotherapie bei multiplem Myelom. Wien Klin Wochenschr 105: 580-584
66. Geissler K, Peschel Ch, Niederwieser D, Goldschmitt J, Hladik F, Fritz A, Ohler L, Bettelheim P, Huber Ch, Lechner K, Höcker P, Kolbe K (1995) Effect of interleukin-3 pretreatment on granulocyte/macrophage colony-stimulating factor induced mobilization of circulating haematopoietic progenitor cells. Br J Haematol 91: 299-305
67. Geissler K, Peschel Ch, Niederwieser D, Strobl H, Goldschmitt J, Öhler L, Bettelheim P, Kahls P, Huber Ch, Lechner K, Höcker P, Kolbe K (1996) Potentiation of granulocyte colony-stimulating factor-induced mobilization of circulating progenitor cells by seven-day pretreatment with interleukin-3. Blood 87: 2732-2739
68. Hinterberger W, Geissler K, Fischer M, Lechner K, Kabrna E (1985) Aplastic anemia: assessment of myeloid progenitor cells in the bone marrow and blood provides prognostic information. Acta Haematol 73: 1-5
69. Gussetis ES, Peristeri J, Kitra V, Liakopoulou T, Kattamis A, Graphakos S (1998) Clinical value of bone marrow cultures in childhood pure red cell aplasia. J Pediatr Hematol Oncol 20: 120-124
70. Issaragrisil S, U-pratya Y, Yimyam M, Pakdeesuwan K, Khuhapinant A, Muangsup W, Pattanapanyasat K (1998) Hematopoietic progenitor cells in the blood and bone marrow in various hematologic disorders. Stem Cells 16 [Suppl 1]: 123-128
71. Podesta M, Piaggio G, Frassoni F, Pitto A, Zikos P, Sessarego M, Abate M, Teresa Van Lint M, Berisso G, Bacigalupo A (1998) The assessment of the hematopoietic reservoir after immunosuppressive therapy or bone marrow transplantation in severe aplastic anemia. Blood 91: 1959-1965
72. Matsuo Y, Iwanaga M, Mori H, Yoshida S, Kawaguchi Y, Yakata Y, Murata K, Nagai K, Jinnai I, Matsuo T, Kuriyama K, Tomonaga M (2000) Recovery of hematopoietic progenitor cells in patients with severe aplastic anemia who obtained good clinical response with a combination therapy of immunosuppressive agents and recombinant human granulocyte colony-stimulating factor. Int J Hematol 72: 37-43
73. Rabbits TH (1994) Chromosomal translocations in human cancer. Nature 372: 143-149
74. Grimwade D, Walker H, Oliver F, Wheatley K, Harrison C, Harrison G, Rees J, Hann I, Stevens R, Burnett A, Goldstone A (1998) The importance of diagnostic cytogenetics on outcome in AML: analysis of 1,612 patients entered into the MRC AML 10 trial. Blood 92: 2322-2333
75. Rowley JD (1999) The role of chromosome translocations in leukemogenesis. Seminars in Hematology 36 [Suppl 7]: 59-72
76. Bonnet D, Dick JE (1997) Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nature Med 3: 730-735
77. Senn JS, McCulloch EA, Till JE (1967) Comparison of colony-forming ability of normal and leukaemic human marrow in cell culture. Lancet 16: 597-598
78. Moore MAS, Metcalf D (1973) Cytogenetic analysis of human acute and chronic myeloid leukemia cells cloned in agar culture. Int J Cancer 11: 143-152
79. Moore MAS, Spitzer G, Williams N, Metcalf D, Buckley J (1974) Agar culture studies in 127 cases of untreated acute leukemia: the prognostic value of reclassification of leukemia according to in vitro growth characteristics. Blood 44: 1-18
80. Spitzer G, Dicke KA, Hehan EA, Smith T, McCredie KB, Barlogie B, Freireich EJ (1976) A simplified in vitro classification for prognosis in adult acute leukemia: the application of in vitro results in remission-predictive models. Blood 48: 795-807
81. Löwenberg B, van Putten WLJ, Touw IP, Delwel R, Santini V (1993) Autonomous proliferation of leukemic cells in vitro as a determinant of prognosis in adult acute myeloid leukemia. N Engl J Med 328: 614-619
82. Hunter AE, Rogers SY, Roberts IAG, Barrett AJ, Russell N (1993) Autonomous growth of blast cells is associated with reduced survival in acute myeloblastic leukemia. Blood 82: 899-903
83. del Canizo MC, Brufau A, Almeida J, Galende J, Garcia Marcos MA, Mota A, Garcia R, Fernandez Calvo J, Ramos F, Fisac P, Orfao A, San Miguel JF (1998) In vitro growth in acute myeloblastic leukemia: relationship with other clinico-biological characteristics of the disease. Br J Haematol 103: 137-142
84. Öhler L, Berer A, Aletaha D, Kabrna E, Heinze G, Streubel B, Fonatsch C, Haas OA, Lechner K, Geissler K (2001) Cytogenetic risk groups in acute myeloid leukaemia differ greatly in their semi-solid colony growth. Brit J Haematol 113: 120-125
85. Bennett JM, Cartovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1982) Proposals for the classification of myelodysplastic syndroms. Br J Haematol 51: 189-199
86. Dupont JM, Fontenay-Roupie M, Dupuy O, Lebarr A, Le Tessier D, Auvinet P, Rabineau D, Fichelson S (1998) Fluorescence in situ hybridization on methylcellulose cultured hematopoietic stem cells from myelodysplastic syndromes. Cancer Genetics and Cytogenetics 101: 12-15
87. Ruutu T, Partanen S, Litula R, Teerenhovi L, Knuutila S (1984) Erythroid and granulocyte-macrophage colony formation in myelodysplastic syndromes. Scand J Haematol 32: 395-402
88. Tennant GB, Jacobs A, Bailey-Wood R (1986) Peripheral blood granulocyte-macrophage progenitors in patients with the myelodysplastic syndromes. Exp Hematol 14: 1063-1068
89. Geissler K, Hinterberger W, Jager U, Bettelheim P, Neumann E, Haas O, Ambros P, Chott A, Radaszkiewicz T, Lechner K (1988) Deficiency of pluripotent hemopoietic progenitor cells in myelodysplastic syndromes. Blut 57: 45-49
90. Geissler K, Hinterberger W, Bettelheim P, Haas O, Lechner K (1988) Colony growth characteristics in chronic myelomonocytic leukemia. Leuk Res 12: 373-377
91. Shih LY, Chiu WF, Lee CT (1991) Diagnostic and prognostic values of in vitro culture growth patterns of marrow granulocyte-macrophage progenitors in patients with myelodysplastic syndrome. Leukemia 12: 1092-1098
92. Francis GE, Miller EJ, Wonke B, Wing MA, Berney JJ, Hoffbrand AV (1983) Use of bone-marrow culture in prediction of acute leukaemic transformation in preleukaemia. Lancet 25: 1409-1412
93. Ruutu T, Partanen S, Litula R, Teerenhovi L, Knuutila S (1984) Erythroid and granulocyte-macrophage colony formation in myelodysplastic syndromes. Scand J Haematol 32: 395-402
94. Raymakers R, Preijers F, Boezeman J, Rutten E, De Witte T (1994) Prognostic implications of bone marrow culturing in myelodysplastic syndrome: a retrospective analysis. Leuk Lymphoma 14: 111-120
95. Tefferi A (2001) Chronic myeloid disorders: classification and treatment overview. Semin Hematol 38: 1-4
96. Nowell PC, Hungerford PA (1960) A minutes chromosome in human granulocytic leukemia. Science 132: 1497
97. Carella AM, Frassoni F, Melo J, Sawyers C, Eaves C, Eaves A, Apperley J, Tura S, Hehlmann R, Reiffers J, Lerma E, Goldman J (1997) New insights in biology and current therapeutic options for patients with chronic myelogenous leukemia. Haematologica 82: 478-495
98. 1) chromosome. Science 174: 1134-1136
99. Lechner K, Geissler K, Gisslinger H (1999) Polycythaemia vera - diagnosis and therapy. Wien Klin Wochenschr 111: 582-589
100. Weinberg RS (1997) In vitro erythropoiesis in polycythemia vera and other myeloproliferative disorders. Semin Hematol 34: 64-69
101. Lutton JD, Levere RD (1979) Endogenous erythroid colony formation by peripheral blood mononuclear cells from patients with myelofibrosis and polycythemia vera. Acta Haematol 62: 94-99
102. de Wolf JT, Beentjes JA, Esselink MT, Smit JW, Halie RM, Clark SC, Vellenga E (1989) In polycythemia vera human interleukin 3 and granulocyte- macrophage colony-stimulating factor enhance erythroid colony growth in the absence of erythropoietin. Exp Hematol 17: 981-983
103. Dai CH, Krantz SB, Means RT Jr, Horn ST, Gilbert HS (1991) Polycythemia vera blood burst-forming units-erythroid are hypersensitive to interleukin-3. J Clin Invest 87: 391-396
104. Dai CH, Krantz SB, Dessypris EN, Means RT Jr, Horn ST, Gilbert HS (1992) Polycythemia vera. II. Hypersensitivity of bone marrow erythroid, granulocyte-macrophage, and megakaryocyte progenitor cells to interleukin-3 and granulocyte-macrophage colony-stimulating factor. Blood 80: 891-899
105. Mirza AM, Ezzat S, Axelrad AA (1997) Insulin-like growth factor binding protein-1 is elevated in patients with polycythemia vera and stimulates erythroid burst formation in vitro. Blood 89: 1862-1869
106. Weinberg RS, Worsley A, Gilbert HS, Cuttner J, Berk PD, Alter BP (1989) Comparison of erythroid progenitor cell growth in vitro in polycythemia vera and chronic myelogenous leukemia: only polycythemia vera has endogenous colonies. Leuk Res 13: 331-338
107. Biljanovic-Paunovic L, Ruvidic R, Pavlovic-Kentera V (1990) Endogenous BFU-E in peripheral blood in diagnosis of polycythemia vera. Eur J Haematol 45: 262-266
108. Shih LY, Lee CT (1994) Identification of masked polycythemia vera from patients with idiopathic marked thrombocytosis by endogenous erythroid colony assay. Blood 83: 744-748
109. Michiels JJ, Juvonen E (1997) Proposal for revised diagnostic criteria of essential thrombocythemia and polycythemia vera by the Thrombocythemia Vera Study Group. Semin Thromb Hemost 23: 339-347
110. Michiels JJ, Thiele J (2002) Clinical and pathological criteria for the diagnosis of essential thrombocythemia, polycythemia vera, and idiopathic myelofibrosis (agnogenic myeloid metaplasia). Int J Hematol 76: 133-145
111. Kimura H, Ishibashi T, Sato T, Matsuda S, Uchida T, Kariyone S (1987) Megakaryocytic colony formation (CFU-Meg) in essential thrombocythemia: quantitative and qualitative abnormalities of bone marrow CFU-Meg. Am J Hematol 24: 23-30
112. Mazur EM, Cohen JL, Bogart L (1988) Growth characteristics of circulating hematopoietic progenitor cells from patients with essential thrombocythemia. Blood 71: 1544-1550
113. Han ZC, Briere J, Abgrall JF, Sensebe L, Parent D, Guern G (1989) Characteristics of megakaryocyte colony formation in normal individuals and in primary thrombocythemia: studies using an optimal cloning system. Exp Hematol 17: 46-52
114. Ciaudo M, Hadjez JM, Teyssandier I, Coly E, Zittoun R, Marie JP (1998) Prognostic and diagnostic value of endogenous erythroid colony formation in essential thrombocythemia. Hematol Cell Ther 40: 171-174
115. Mi JQ, Blanc-Jouvan F, Wang J, Sotto MF, Cousin F, Castinel A, Chauvet M, Sotto JJ, Polack B, Mossuz P (2001) Endogenous megakaryocytic colony formation and thrombopoietin sensitivity of megakaryocytic progenitor cells are useful to distinguish between essential thrombocythemia and reactive thrombocytosis. J Hematother Stem Cell Res 10: 405-409
116. Barosi G, Berzuini C, Liberato LN, Costa A, Polino G, Ascari E (1988) A prognostic classification of myelofibrosis with myeloid metaplasma. Br J Haematol 70: 397-401
117. Smith BD, Moltinero AR (2001) Biology an management of idiopathic myelofibrosis. Curr Opin Oncol 13: 91-94
118. Le Bousse-Kerdiles MC, Chevillard S, Charpentier A, Romquin N, Clay D, Smadja-Joffe F, Praloran V, Dupriez B, Demory JL, Jasmin C, Martyre MC (1996) Differential expression of transforming growth factor-β, basic fibroblast growth factor, and their receptors in CD34+ hematopoietic progenitor cells from patients with myelofibrosis and myeloid metaplasia. Blood 88: 4534-4546
119. Chervenick PA (1973) Increase in circulating stem cells in patients with myelofibrosis. Blood 41: 67-71
120. Hibbin JA, Njoku OS, Matutes E, Lewis SM, Goldman JM (1984) Myeloid progenitor cells in the circulation of patients with myelofibrosis and other myeloproliferative disorders. Br J Haematol 57: 495-503
121. Charbord P, Neel H (1985) Density of granulomonocytic colony-forming cells (GM-CFC's) in myelofibrosis. Scand J Haematol 35: 394-398
122. Barosi G (1999) Myelofibrosis with myeloid metaplasia: diagnostic definition and prognostic classification for clinical studies and treatment guidelines. J Clin Oncol 17: 2954-2970
123. Andreasson B, Swolin B, Kutti J (2002) Patients with idiopathic myelofibrosis show increased CD34+ cell concentrations in peripheral blood compared to patients with polycythaemia vera and essential thrombocythaemia. Eur J Haematol 68: 189-193
124. Barosi G, Viarengo G, Pecci A, Rosti V, Piaggio G, Marchetti M, Frassoni F (2001) Diagnostic and clinical relevance of the number of circulating CD34(+) cells in myelofibrosis with myeloid metaplasia. Blood 98: 3249-3255