Generation of antibodies to the urokinase receptor (uPAR) by DNA immunization of uPAR knockout mice: Membrane-bound uPAR is not required for an antibody response

Scandinavian Journal of Immunology

Volumn 58, Issue 3, 2003, Pages 298-305

  Pass, J ^a   Gardsvoll H ^a   Lund, L R ^a   Dano K ^a   Hoyer Hansen G ^a  

^a FINSEN LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ANTISERUM; DNA; MONOCLONAL ANTIBODY; RECOMBINANT RECEPTOR; UROKINASE RECEPTOR;

ANIMAL CELL; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIBODY TITER; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; COMPARATIVE STUDY; CONTROLLED STUDY; FEMALE; GENOTYPE; HUMORAL IMMUNITY; IMMUNIZATION; INJECTION; KNOCKOUT MOUSE; MEMBRANE BINDING; MOUSE; NONHUMAN; PRIORITY JOURNAL; TH2 CELL; WILD TYPE;

ANIMALS; ANTIBODIES; ANTIBODY FORMATION; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; COS CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GENETIC VECTORS; HUMANS; IMMUNIZATION; IMMUNOGLOBULIN G; MICE; MICE, KNOCKOUT; PLASMIDS; RECEPTORS, CELL SURFACE; TRANSFECTION; VACCINES, DNA;

EID: 0042412332     PISSN: 03009475     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3083.2003.01299.x     Document Type: Article

References (38)

1. McCluskie MJ, Brazolot Millan CL, Gramzinski RA et al. Route and method of delivery of DNA vaccine influence immune responses in mice and non-human primates. Mol Med 1999;5:287-300.
2. Tang DC, DeVit M, Johnston SA. Genetic immunization is a simple method for eliciting an immune response. Nature 1992;356:152-4.
3. Gårdsvoll H, Solberg H, Danø K, Høyer-Hansen G. Generation of high-affinity rabbit polydonal antibodies to the murine urokinase receptor using DNA immunization. J Immunol Methods 2000;234:107-16.
4. Montgomery DL, Ulmer JB, Donelly JJ, Liu MA. DNA Vaccines. Pharmacol Ther 1997;74:195-205.
5. Condon C, Watkins SC, Celluzzi CM, Thompson K, Falo LD Jr. DNA-based immunization by in vivo transfection of dendritic cells. Nat Med 1996;2:1122-8.
6. Doe B, Selby M, Barnett S, Baenziger J, Walker CM. Induction of cytotoxic T iymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells. Proc Natl Acad Sci USA 1996;93:8578-83.
7. Fu TM, Ulmer JB, Caulfield MJ et al. Priming of cytotoxic T lymphocytes by DNA vaccines: requirement for professional antigen presenting cells and evidence for andgen transfer from myocytes. Mol Med 1997;3:362-71.
8. Porgador A, Irvine KR, Iwasaki A, Barber BH, Restifo NP, Germain RN. Predominant role for directly transfected dendritic cells in antigen presentation to CD8+ T cells after gene gun immunization. J Exp Med 1998;188:1075-82.
9. Behrendt N, Stephens RW. The urokinase receptor. Fibrinolysis & Proteolysis 1998;12:191-204.
10. Kristensen P, Eriksen J, Blasi F, Danø K. Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract. J Cell Biol 1991;115:1763-71.
11. Claesson MH, Endel B, Ulrik J, Pedersen LO, Skov S, Buus S. Antibodies directed against monomorphic and evolutionary conserved self epitopes may be generated in 'knock-out' mice. Development of monoclonal antibodies directed against monomorphic MHC class I determinants. Scand J Immunol 1994;40:257-64.
12. Declerck PJ, Verstreken M, Collen D. Immunoassay of murine t-PA, u-PA and PAI-1 using monoclonal antibodies raised in gene-inactivated mice. Thromb Haemost 1995;74:1305-9.
13. Declerck PJ, Carmeliet P, Verstreken M, De Cock F, Collen D. Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice. J Biol Chem 1995;270:8397-400.
14. Ferrero E, Vettoretto K, Bondanza A et al. uPA/uPAR system is active in immature dendritic cells derived from CD14+CD34+ precursors and is down-regulated upon maturation. J Immunol 2000;164:712-8.
15. Woodhead VE, Stonehouse TJ, Binks MH et al. Novel molecular mechanisms of dendritic cell-induced T cell activation. Int Immunol 2000;12:1051-61.
16. Roldan AL, Cubellis MV, Masucci MT et al. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. EMBO J 1990;9:467-74.
17. Hartikka J, Sawdey M, Cornefert-Jensen F et al. An improved plasmid DNA expression vector for direct injection into skeletal muscle. Hum Gene Ther 1996;7:1205-17.
18. Gårdsvoll H, Danø K, Ploug M. Mapping part of the functional epitope for ligand binding on the receptor for urokinase-rype plasminogen activator by site-directed mutagenesis. J Biol Chem 1999;274:37995-8003.
19. Bugge TH, Suh TT, Flick MJ et al. The receptor for urokinase-type plasminogen activator is not essential for mouse development or fertility. J Biol Chem 1995;270:16886-94.
20. Rehbinder C, Baneux P, Forbes D et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, gerbil, guinea pig and rabbit experimental units. Report of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Health accepted by the FELASA Board of Management, November 1995. Lab Anim 1996;30:193-208.
21. Solberg H, Løber D, Eriksen J et al. Identification and characterization of the murine cell surface receptor for the urokinase-type plasminogen activator. Eur J Biochem 1992;205:451-8.
22. Rønne E, Behrendt N, Ellis V, Ploug M, Danø K, Høyer-Hansen G. Cell-induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2-terminal domain of the urokinase receptor. FEBS Lett 1991;288:233-6.
23. Boyle JS, Koniaras C, Lew AM. Influence of cellular location of expressed antigen on the efficacy of DNA vaccination: cyrotoxic T lymphocyte and antibody responses are suboptimal when antigen is cytoplasmic after intramuscular DNA immunization. Int Immunol 1997;9:1897-906.
24. Drew DR, Lightowlers M, Strugnell RA. Humoral immune responses to DNA vaccines expressing secreted, membrane bound and non-secreted forms of the Tania ovis 45W antigen. Vaccine 2000;18:2522-32.
25. Zinkernagel RM. Localization dose and time of antigens determine immune reactivity. Semin Immunol 2000;12:163-71.
26. Norman J, Hartikka J, Strauch P, Manthorpe M. Adjuvants for plasmid DNA vaccines. In: Lowrie DB, Whalen RG, eds. DNA Vaccines: Methods and Protocols. Humana Press, 2000, 185-96.
27. Barry MA, Barry ME, Johnston SA. Production of monoclonal antibodies by genetic immunization. Biotechniques 1994;16: (616-8), 620.
28. Chowdhury PS, Gallo M, Pastan I. Generation of high titer antisera in rabbits by DNA immunization. J Immunol Methods 2001;249:147-54.
29. Moonsom S, Khunkeawla P, Kasinrerk W. Production of polyclonal and monoclonal antibodies against CD54 molecules by intrasplenic immunization of plasmid DNA encoding CD54 protein. Immunol Lett 2001;76:25-30.
30. Smooker PM, Steeper KR, Drew DR, Strugnell RA, Spithill TW. Humoral responses in mice following vaccination with DNA encoding glutathione S-transferase of Fasciola hepatica: effects of mode of vaccination and the cellular compartment of antigen expression. Parasite Immunol 1999;21:357-64.
31. Wang L, Menting JG, Black CG et al. Differences in epitope recognition, isotype and titer of antisera to Plasmodium falciparum merozoite surface protein 4 raised by different modes of DNA or protein immunization. Vaccine 2000;19:816-24.
32. Kamath AT, Hanke T, Briscoe H, Britton WJ. Co-immunization with DNA vaccines expressing granulocyte-macrophage colony-stimulating factor and mycobacterial secreted proteins enhances T-cell immunity, but not protective efficacy against Mycobacterium tuberculosis. Immunology 1999;96:511-6.
33. Krieg AM, Yi AK, Matson S et al. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 1995;374:546-9.
34. Sato Y, Roman M, Tighe H et al. Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science 1996;273:352-4.
35. Martin-Orozco E, Kobayashi H, Van Uden J, Nguyen MD, Kornbluth RS, Raz E. Enhancement of antigen-presenting cell surface molecules involved in cognate interactions by immunostimulatory DNA sequences. Int Immunol 1999;11:1111-8.
36. Robinson HL, Torres CA. DNA Vaccines. Semin Immunol 1997;9:271-83.
37. Bianchi E, Bender JR, Blasi F, Pardi R. Through and beyond the wall: late steps in leukocyte transendothelial migration. Immunol Today 1997;18:586-91.
38. Engelholm LH, Behrendt N. Differential binding of urokinase and peptide antagonists to the urokinase receptor: evidence from characterization of the receptor in four primate species. Biol Chem 2001;382:435-42.