Biochemical characterization of the active heterodimer form of human heparanase (Hpa1) protein expressed in insect cells

Biochemical Journal

Volumn 373, Issue 2, 2003, Pages 423-435

  McKenzie, Edward ^a   Young, Kathryn ^a   Hircock, Margaret ^a   Bennett, James ^a   Bhaman, Maina ^a   Felix, Robert ^a   Turner, Paul ^a   Stamps, Alasdair ^a   McMillan, David ^a   Saville, Giles ^a   Ng, Stanley ^a   Mason, Sean ^a   Snell, Daniel ^a   Schofield, Darren ^a   Gong, Haiping ^a   Townsend, Reid ^a   Gallagher, John ^b   Page, Martin ^c   Parekh, Raj ^a   Stubberfield, Colin ^a  

^a Oxford GlycoSciences   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c Oncogene Sci Inc Pharmaceuticals   (United Kingdom)

Author keywords

Heparan sulphate; Heparanase; Heparanase heterodimer

Indexed keywords

BIOCHEMISTRY; CELLS; CHROMATOGRAPHY; PATHOLOGY; PH EFFECTS; POLYPEPTIDES;

INSECT CELLS;

PROTEINS;

HEPARANASE; PROTEOHEPARAN SULFATE; RECOMBINANT ENZYME;

ACIDITY; ANGIOGENESIS; ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BASEMENT MEMBRANE; CELL PROLIFERATION; EMBRYO DEVELOPMENT; ENZYME ANALYSIS; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; INFLAMMATION; INSECT CELL; METASTASIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

AMIDOHYDROLASES; ANIMALS; BACULOVIRIDAE; BLOOD PLATELETS; CHROMATOGRAPHY, AFFINITY; DIMERIZATION; FIBRINOLYTIC AGENTS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; GLUCURONIDASE; HEPARIN; HEPARITIN SULFATE; HUMANS; IMMUNOENZYME TECHNIQUES; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; RECOMBINANT PROTEINS; SEQUENCE DELETION; SPODOPTERA; TUMOR CELLS, CULTURED;

ANIMALIA; INSECTA; MAMMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0042366191     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030318     Document Type: Article

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