Contribution of conserved amino acids at the dimeric interface to the conformational stability and the structural integrity of the active site in ketosteroid isomerase from Pseudomonas putida biotype B

Journal of Biochemistry

Volumn 134, Issue 1, 2003, Pages 101-110

  Nam, Gyu Hyun ^a   Kim, Do Hyung ^a,b   Ha, Nam Chul ^a   Jang, Do Soo ^a   Yun, Young Sung ^a   Hong, Bee Hak ^a   Oh, Byung Ha ^a   Choi, Kwan Yong ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Conserved interface residues; Dimeric enzyme; Dimeric interactions; Ketosteroid isomerase; Site directed mutagenesis

Indexed keywords

ALANINE; AMINO ACID; BACTERIAL ENZYME; DIMER; STEROID DELTA ISOMERASE;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; FLUORESCENCE; NONHUMAN; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STABILITY; PSEUDOMONAS PUTIDA; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; AMINO ACIDS; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ENZYME STABILITY; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; STEROID ISOMERASES; THERMODYNAMICS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0042320440     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg117     Document Type: Article

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