A 50S ribosomal subunit precursor particle is a substrate for the ErmC methyltransferase in Staphylococcus aureus cells

Current Microbiology

Volumn 46, Issue 6, 2003, Pages 453-460

  Champney, W Scott ^a   Chittum, Harold S ^a   Tober, Craig L ^a  

^a East Tennessee State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ERMC METHYLTRANSFERASE; ERYTHROMYCIN; METHYLTRANSFERASE; RIBOSOME PROTEIN; RNA 23S; S ADENOSYLMETHIONINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; DRUG BINDING; ENZYME ACTIVITY; ENZYME SUBSTRATE; NONHUMAN; NUCLEASE PROTECTION ASSAY; PRIORITY JOURNAL; RIBOSOME SUBUNIT; RNA ISOLATION; RNA METHYLATION; RNA SEQUENCE; STAPHYLOCOCCUS AUREUS;

ANTI-BACTERIAL AGENTS; ASPERGILLUS NUCLEASE S1; CENTRIFUGATION, DENSITY GRADIENT; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ERYTHROMYCIN; METHYLTRANSFERASES; RIBOSOMES; RNA PRECURSORS; RNA, RIBOSOMAL, 23S; STAPHYLOCOCCUS AUREUS; URIDINE;

ANIMALIA; BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0041671051     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-002-3901-8     Document Type: Article

References (37)

1. Amabile-Cuevas CF, Cardenas-Garcia M, Ludgar M (1995) Antibiotic resistance. Am Sci 83:320-329
2. B resistance phenotype. J Antimicrob Chemother 40:85-90
3. Bussiere DE, Muchmore SW, Dealwis CG, Schluckebier G, Nienaber VL, Edalji RP, Walter KA, Ladror US, Holzman TF, Abad-Zapatero C (1998) Crystal structure of ermC′, an rRNA methyltransferase which mediates antibiotic resistance in bacteria. Biochemistry 37:7103-7112
4. Champney WS (2001) Bacterial ribosomal subunit synthesis: a novel antibiotic target. Curr Drug Targets Infect Disorders 1:19-36
5. Champney WS, Sypherd PS (1970) Absence of thiolated nucleotides in ribosomal RNA of E. coli. Biochem Biophys Res Commun 41:1328-1334
6. Champney WS, Tober CL (1998) Inhibition of translation and 50S ribosomal subunit formation in Staphylococcus aureus cells by eleven different ketolide antibiotics. Curr Microbiol 37:418-425
7. Champney WS, Tober CL (2000) Evernimicin (SCH27899) inhibits both translation and 50S ribosomal subunit formation in Staphylococcus aureus cells. Antimicrob Agents Chemother 44:1413-1417
8. Champney WS, Tober CL, Burdine R (1998) A comparison of the inhibition of translation and 50S ribosomal subunit formation in Staphylococcus aureus cells by nine different macrolide antibiotics. Curr Microbiol 37:412-417
9. Chopra I, Hodgson J, Metcalf B, Poste G (1997) The search for antimicrobial agents effective against bacteria resistant to multiple antibiotics. Antimicrobial Agents Chemother 41:497-503
10. Chu DT, Plattner JJ, Katz L (1996) New directions in antibacterial research. J Med Chem 39:3853-3874
11. Denoya CD, Dubnau D (1987) Site and substrate specificity of the ermC 23S rRNA methyltransferase. J Bacteriol 169:3857-3860
12. Denoya C, Dubnau D (1989) Mono-and dimethylating activities and kinetic studies of the ermC 23S rRNA methyltransferase. J Biol Chem 264:2615-2624
13. Denoya CA, Bechhofer DH, Dubnau D (1986) Translational autoregulation of ermC 23S rRNA methyltransferase expression in Bacillus subtilis. J Bacteriol 168:1133-1141
14. Gold HS, Moellering RC (1996) Antimicrobial-drug resistance. N Engl J Med 335:1445-1453
15. Horinouchi S, Weisblum B (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J Bacteriol 150:804-814
16. Kovalic D, Giannattasio RB, Weisblum B (1995) Methylation of minimalist 23S rRNA sequences in vitro by ermSF (trlA) N-methyltransferase. Biochemistry 34:15838-15844
17. Ludwig W, Kirchof G, Klugbauer N, Weizenegger M, Betzl D, Ehrmann M, Hertel C, Jilg S, Tatzel R, Zitzelsberger H, Liebl S, Hochberger M, Shah J, Lane D, Wallnoef PR (1992) Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+C content. Syst Appl Microbiol 15:487-501
18. Mazzei T, Mini E, Novelli A, Periti P (1993) Chemistry and mode of action of macrolides. J Antimicrob Chemother 31:1-9
19. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
20. Narayanan CS, Dubnau D (1987a) Demonstration of erythromycin-dependent stalling of ribosomes on the ermC leader transcript. J Biol Chem 262:1766-1771
21. Narayanan CS, Dubnau D (1987b) An in vitro study of the translational attenuation model of ermC regulation. J Biol Chem 262:1756-1765
22. Nielsen AK, Douthwaite S, Vester B (1999) Negative in vitro selection identifies the rRNA recognition site for ermE methyltransferase. RNA 5:1034-1041
23. Rahi H, Mothershed CP, Champney WS (1979) Protein synthesis kinetics with ribosomes from temperature-sensitive mutants of Escherichia coli. Biochemistry 24:5482-5489
24. Schlunzen F, Zarivach R, Harms J, Bashan A, Tocilj A, Albrecht R, Yonath A, Franceschi F (2001) Structural basis for the interaction of antibiotics with the peptidyltransferase centre in eubacteria. Nature 413:814-821
25. Shivakumar AG, Dubnau D (1981) Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance. Nucleic Acids Res 9:2549-2562
26. Skinner R, Cundliffe E, Schmidt FJ (1983) Site of action of ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics. J Biol Chem 258:12702-12706
27. Sutcliffe J (1999) Resistance to macrolides mediated by efflux mechanisms. Curr Opin Investig Drugs 1:403-412
28. Thakker-Varia S, Jennsen WD, Moon-McDermott L, Weinstein MP, Dubin DT (1987) Molecular epidemiology of macrolides-lincosamides-streptogramin B resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother 31:735-743
29. 5C967 methytransferase from Escherichia coli. Biochemistry 38:1884-1892
30. Usary J, Champney WS (2001) Erythromycin inhibition of 50S ribosomal subunit formation in Escherichia coli cells. Mol Microbiol 40:951-962
31. Vazquez D (1979) Inhibitors of protein biosynthesis. Berlin: Springer-Verlag
32. Vester B, Douthwaite S (2001) Macrolide resistance conferred by base substitutions in 23S rRNA. Antimicrob Agents Chemother 45:1-12
33. Vester B, Nielsen AK, Hansen LH, Douthwaite S (1998) ErmE methyltransferase recognition elements in RNA substrates. J Mol Biol 282:255-264
34. Villsen ID, Vester B, Douthwaite S (1999) ErmE methyltransferase recognizes features of the primary and secondary structure in a motif within domain V of 23S rRNA. J Mol Biol 286:365-374
35. Weisblum B (1985) Inducible resistance to macrolides, lincosamides and streptogramin type B antibiotics: the resistance phenotype, its biological diversity, and structural elements that regulate expression - a review. J Antimicrob Chemother 16:63-90
36. Weisblum B (1995) Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother 39:577-585
37. Weisblum B, Siddhikol C, Lai CJ, Demohn V (1971) Erythromycin-inducible resistance in Staphylococcus aureus: requirements for induction. J Bacteriol 106:835-847