An automated high-throughput assay for survival of the nematode Caenorhabditis elegans

Free Radical Biology and Medicine

Volumn 35, Issue 6, 2003, Pages 558-565

  Gill, Matthew S ^a   Olsen, Anders ^a   Sampayo, James N ^a   Lithgow, Gordon J ^a  

^a BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

Aging; C. elegans; Compound screen; Free radicals; Life span; Superoxide dismutase catalase mimetic; Survival

Indexed keywords

FLUORESCENT DYE; OXYGEN RADICAL; SUPEROXIDE DISMUTASE;

AGING; ANALYTIC METHOD; ARTICLE; ASSAY; AUTOMATION; CAENORHABDITIS ELEGANS; DRUG SCREENING; FLUORESCENCE; GENE IDENTIFICATION; HEAT STRESS; LIFESPAN; NONHUMAN; OXIDATIVE STRESS; PARASITE SURVIVAL; PRIORITY JOURNAL;

CAENORHABDITIS; CAENORHABDITIS ELEGANS;

EID: 0041326859     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(03)00328-9     Document Type: Article

References (23)

1. Lithgow G.J. Invertebrate gerontology the age mutations of Caenorhabditis elegans . Bioessays. 18:1996;809-815.
2. Lithgow G.J., Walker G.A. Stress resistance as a determinate of C. elegans life span. Mech. Ageing Dev. 123:2002;765-771.
3. Lithgow G.J., White T.M., Melov S., Johnson T.E. Thermotolerance and extended life span conferred by single-gene mutations and induced by thermal stress. Proc. Natl. Acad. Sci. USA. 92:1995;7540-7544.
4. Vanfleteren J.R. Oxidative stress and ageing in Caenorhabditis elegans. Biochem. J. 292:1993;605-608.
5. Barsyte D., Lovejoy D.A., Lithgow G.J. Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans. FASEB J. 15:2001;627-634.
6. Tower J. Aging mechanisms in fruit flies. Bioessays. 18:1996;799-807.
7. Holzenberger M., Dupont J., Ducos B., Leneuve P., Geloen A., Even P.C., Cervera P., Le Bouc Y. IGF-1 receptor regulates life span and resistance to oxidative stress in mice. Nature. 421:2003;182-187.
8. Lithgow G.J., Andersen J.K. The real Dorian Gray mouse. Bioessays. 22:2000;410-413.
9. Tatar M., Khazaeli A.A., Curtsinger J.W. Chaperoning extended life. Nature. 390:1997;30.
10. Yokoyama K., Fukumoto K., Murakami T., Harada S., Hosono R., Wadhwa R., Mitsui Y., Ohkuma S. Extended longevity of Caenorhabditis elegans by knocking in extra copies of hsp70F, a homolog of mot-2 (mortalin)/mthsp70/Grp75. FEBS Lett. 516:2002;53-57.
11. Walker G.A., Lithgow G.J. Life span extension in C. elegans by a molecular chaperone dependent upon insulin-like signals. Aging Cell. 2:2003;131-139.
12. Sampayo J.N., Jenkins N.L., Lithgow G.J. Using stress resistance to isolate novel longevity mutations in Caenorhabditis elegans. Ann. NY Acad. Sci. 908:2000;324-326.
13. Munoz M.J., Riddle D.L. Positive selection of Caenorhabditis elegans mutants with increased stress resistance and longevity. Genetics. 163:2003;171-180.
14. Melov S., Ravenscroft J., Malik S., Gill M.S., Walker D.W., Clayton P.E., Wallace D.C., Malfroy B., Doctrow S.R., Lithgow G.J. Extension of life span with superoxide dismutase/catalase mimetics. Science. 289:2000;1567-1569.
15. Harrington L.A., Harley C.B. Effect of vitamin E on life span and reproduction in Caenorhabditis elegans. Mech. Ageing Dev. 43:1988;71-78.
16. Kang H.L., Benzer S., Min K.T. Life extension in Drosophila by feeding a drug. Proc. Natl. Acad. Sci. USA. 99:2002;838-843.
17. Roth B.L., Poot M., Yue S.T., Millard P.J. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl. Environ. Microbiol. 63:1997;2421-2431.
18. Sulston J., Hodgkin J. Methods. Wood W.B. The nematode Caenorhabditis elegans. 1988;587-606 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Lithgow G.J., White T.M., Hinerfeld D.A., Johnson T.E. Thermotolerance of a long-lived mutant of Caenorhabditis elegans. J. Gerontol. 49:1994;B270-B276.
20. Johnson T.E., Henderson S., Murakami S., de Castro E., de Castro S.H., Cypser J., Rikke B., Tedesco P., Link C. Longevity genes in the nematode Caenorhabditis elegans also mediate increased resistance to stress and prevent disease. J. Inherit. Metab. Dis. 25:2002;197-206.
21. Yang Y., Wilson D.L. Isolating aging mutants a novel method yields three strains of the nematode Caenorhabditis elegans with extended life spans . Mech. Ageing Dev. 113:2000;101-116.
22. Fabian T.J., Johnson T.E. Total RNA, rRNA, and poly(A) + RNA abundances during aging in Caenorhabditis elegans. Mech. Ageing Dev. 83:1995;155-170.
23. Link C.D., Cypser J.R., Johnson C.J., Johnson T.E. Direct observation of stress response in Caenorhabditis elegans using a reporter transgene. Cell Stress Chaperones. 4:1999;235-242.