A new one-step RT-PCR method for virus quantitation in murine AIDS

Journal of Virological Methods

Volumn 110, Issue 1, 2003, Pages 81-90

  Casabianca, Anna ^a   Orlandi, Chiara ^a   Fraternale, Alessandra ^a   Magnani, Mauro ^a  

^a UNIVERSITY OF URBINO   (Italy)

Author keywords

Defective virus expression; Ecotropic virus expression; Murine AIDS; Standard curve; Virus quantitation

Indexed keywords

BETA ACTIN; COMPLEMENTARY DNA; DIDANOSINE; VIRUS DNA; VIRUS PROTEIN; VIRUS RNA; ZIDOVUDINE;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; HELPER VIRUS; LYMPH NODE; MOLECULAR CLONING; MOUSE; MURINE LEUKEMIA VIRUS; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RODENT DISEASE; SPLEEN; VIRUS DETECTION;

ANIMALIA; MURINAE; MURINE LEUKEMIA VIRUS;

EID: 0038580793     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(03)00104-6     Document Type: Article

References (42)

1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
2. Aziz D.C., Hanna Z., Jolicoeur P. Severe immunodeficiency disease induced by a defective murine leukemia virus. Nature. 338:1989;505-508.
3. Basham T., Rios C.D., Holdener T., Merigan T.C. Zidovudine (AZT) reduces virus titer, retards immune dysfunction, and prolongs survival in LP-BM5 murine induced immunodeficiency model. J. Infect. Dis. 161:1990;1006-1009.
4. Casabianca A., Magnani M. A p12 gag gene homologue is present in the mouse genome. Biochem. Mol. Biol. Int. 32:1994;691-696.
5. Casabianca A., Vallanti G., Magnani M. Competitive PCR for quantification of BM5d proviral DNA in mice with AIDS. J. Clin. Microbiol. 36:1998;2371-2374.
6. Chattopadhyay S.K., Morse H.C. III, Makino M., Ruscetti S.K. Defective virus is associated with induction of murine retrovirus-induced immunodeficiency syndrome. Proc. Natl. Acad. Sci. USA. A86:1989;3862-3866.
7. Chattopadhyay S.K., Sengupta D.N., Fredickson T.N., Morse H.C. III, Hartley J.W. Characteristics and contributions of defective, ecotropic and cell focus forming viruses involved in a retrovirus-induced immunodeficiency syndrome of mice. J. Virol. 65:1991;4232-4241.
8. Chelly J., Kaplan J.C., Maire P., Gautron S., Kahn A. Transcription of the dystrophin gene in human muscle and non-muscle tissues. Nature. 333:1988;858-860.
9. Chen Z., Smithberger J., Sun B., Eggerman T.L. Prevention of heteroduplex formation in mRNA quantitation by reverse transcription-polymerase chain reaction. Anal. Biochem. 266:1998;230-232.
10. Cheung S.C., Chattopadhyay S.K., Hartley J.W., Morse H.C. III, Pitha P.M. Aberrant expression of cytokine genes in peritoneal macrophages from mice infected with LP-BM5 MuLV, a murine model of AIDS. J. Immunol. 146:1991;121-127.
11. Desforges B., Hatin I., Akarid K., Sinet M. Competitive reverse transcription-polymerase chain reaction for quantifying murine AIDS virus. J. Virol. Methods. 62:1996;161-168.
12. Dukas K., Sarfati P., Vayasse N., Pradayrol L. Quantitation of changes in the expression of multiple genes by simultaneous chain reaction. Anal. Biochem. 215:1993;66-72.
13. Eggert A., Brodeur G.M., Ikegaki N. Relative quantitative RT-PCR protocol for TrkB expression in neuroblastoma using GAPD as an internal control. BioTechniques. 28:2000;681-691.
14. Fraternale A., Casabianca A., Rossi L., Chiarantini L., Brandi G., Aluigi G., Schiavano G.F., Magnani M. Inhibition of murine AIDS by combination of AZT and dideoxycytidine 5′-triphosphate. J. Acquired Immune Defic. Syndr. Hum. Retrovirol. 12:1996;164-173.
15. Fraternale A., Tonelli A., Casabianca A., Vallanti G., Chiarantini L., Schiavano G.F., Benatti U., De Flora A., Magnani M. Role of macrophage protection in the development of murine AIDS. J. Acquired Immune Defic. Syndr. 21:1999;81-89.
16. Fraternale A., Casabianca A., Tonelli A., Vallanti G., Chiarantini L., Celeste A.G., Magnani M. Inhibition of murine AIDS by alternative administration of azidothymidine and Fludarabine monophosphate. J. Acquired Immune Defic. Syndr. 23:2000;209-220.
17. Fraternale A., Tonelli A., Casabianca A., Chiarantini L., Schiavano G.F., Celeste A.G., Magnani M. New treatment protocol including lympholytic and antiretroviral drugs to inhibit murine AIDS. J. Acquired Immune Defic. Syndr. 23:2000;107-113.
18. Fraternale A., Casabianca A., Tonelli A., Chiarantini L., Brandi G., Magnani M. New drug combination for the treatment of murine AIDS and macrophage protection. Eur. J. Clin. Invest. 31:2001;248-252.
19. Gazzinelli R.T., Giese N.A., Morse H.C. III. In vivo treatment with interleukin 12 protects mice from immune abnormalities observed during murine acquired immunodeficiency syndrome (MAIDS). J. Exp. Med. 50:1994;2199-2208.
20. Harting I., Wiesner R.J. Quantification of transcript-to-template ratio as a measure of gene expression using RT-PCR. BioTechniques. 23:1997;450-455.
21. Harvie P., Omar R.F., Dusserre N., Lansac N., Désormeaux A., Goude P., Simard M., Tremblay M., Beauchamp D., Bergeron M.G. Ribavirin potentiates the efficacy and toxicity of 2′,3′-dideoxyinosine in the murine acquired immunodeficiency syndrome model. J. Pharmacol. Exp. Ther. 279:1996;1009-1017.
22. Hayashi H., Matsubara H., Yokota T., Kuwabara I., Kanno M., Kosebi H., Isono K. Molecular cloning and characterization of the gene encoding mouse melanoma antigen by cDNA library transfection. J. Immunol. 149:1992;1223-1229.
23. Herr W. Nucleotide sequence of AKV murine leukemia virus. J. Virol. 49:1984;471-478.
24. Huang M., Simard C., Jolicoeur P. Immunodeficiency and clonal growth of target cells induced by helper-free defective retroviruses. Science. 246:1989;1614-1617.
25. Jolicoeur P. Murine acquired immunodeficiency syndrome (MAIDS): an animal model to study AIDS pathogenesis. FASEB J. 5:1991;2398-2405.
26. gag sequence to a MAIDS-inducing sequence by frameshift mutation. J. Virol. 70:1996;6405-6409.
27. Laterjet R., Duplan J.F. Experiments and discussion on leukemogenesis by cell-free extracts of radiation-induced leukemia in mice. Int. J. Radiat. Biol. 5:1962;339-344.
28. Li M., Huang X., Zhu Z., Gorelik E. Sequence and insertion sites of murine melanoma-associated retrovirus. J. Virol. 73:1999;9178-9186.
29. Lowy D.R., Rowe W.P., Teich N., Hartley J.W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 174:1971;155-156.
30. Magnani M., Casabianca A., Fraternale A., Brandi G., Chiarantini L., Benatti U., Scarfì S., Millo E., De Flora A. Inhibition of murine AIDS by a new azidothymidine homodinucleotide. J. Acquired Immune Defic. Syndr. Hum. Retrovirol. 17:1998;189-195.
31. Morse H.C. III, Chattopadhyay S.K., Makino M., Fredickson T.N., Hugin A.W., Hartley J.W. Retrovirus-induced immunodeficiency in the mouse: MAIDS as a model for AIDS [editorial]. AIDS. 6:1992;607-621.
32. Mosier D.E., Yetter R.A., Morse H.C. III. Functional T lymphocytes are required for a murine retrovirus-induced immunodeficiency disease (MAIDS). J. Exp. Med. 165:1987;1737-1742.
33. Mulder J., McKinney N., Christopherson C., Sninsky J., Greenfield L., Kwok S. Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection. J. Clin. Microbiol. 32:1994;292-300.
34. Pesce A.M., Dell'Anna L., Fiorilli M., Carbonari M. Optimization of quantitative co-amplification of β-globin and HIV-1 sequence. Trend Genet. 12:1996;447-448.
35. Plunkett W., Gandhi V., Huang P., Robertson L.E., Yang L.Y., Gregoire V., Estey E., Keating M.J. Fludarabine: pharmacokinetics, mechanisms of action, and rationales for combination therapies. Semin. Oncol. 20:1993;2-12.
36. Pozsgay J.M., Reid S., Pitha P.M. Dissociation between lymphoproliferative responses and virus replication in mice with different sensitivities to retrovirus-induced immunodeficiency. J. Virol. 67:1993;980-988.
37. Sanger F., Nicklend S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
38. Siebert P.D. Quantitative RT-PCR. Kochanowski B., Reischl U. Quantitative PCR Protocol. 1999;61-85 Humana Press, Totowa, NJ.
39. Sommadossi J.-P. Cellular nucleoside pharmacokinetics and pharmacology: a potentially important determinant of antiretroviral efficacy. AIDS. 12:(Suppl. 3):1998;1-8.
40. Swiderski C.E., Klei T.R., Horohov D.W. Quantitative measurement of equine cytokine mRNA expression by polymerase chain reaction using target-specific standard curves. J. Immunol. Methods. 222:1999;155-169.
41. Tayar L., Higo K., Kubo Y., Wang Y., Lu L.M., Zhang F., Yawatani Y., Wang L., Ono T., Maeda M., Sakai H., Ishimoto A. Induction of B-cell lymphoma in BALB/c nude mice with an ecotropic, B-tropic helper virus present in the murine AIDS virus stock. J. Virol. 73:1999;1640-1644.
42. Tokunaga K., Tanigushi H., Yoda K., Shimizu M., Sakiyama S. Nucleotide sequence of a full-length cDNA for mouse cytoskeletal beta-actin mRNA. Nucleic Acids Res. 14:1986;2829.