Quantification of transcript-to-template ratios as a measure of gene expression using RT-PCR

BioTechniques

Volumn 23, Issue 3, 1997, Pages 450-455

  Harting, Inga ^a   Wiesner, Rudolf J ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME C OXIDASE; MESSENGER RNA; MITOCHONDRIAL DNA; RIBOSOME RNA;

ARTICLE; DNA TEMPLATE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HEART VENTRICLE; HUMAN; HUMAN TISSUE; MUSCLE BIOPSY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0030884708     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233st01     Document Type: Article

References (12)

1. Birmboim, H.C. 1993. Extraction of high molecular weight RNA and DNA from cultured mammalian cells. Methods Enzymol. 216:154-160.
2. Cantatore, P., M.N. Gadaleta and C. Saccone. 1984. Determination of some mitochondrial RNAs concentration in adult rat liver. Biochem. Biophys. Res. Commun. 118:284-291.
3. Garstka, H., M. Fäcke, J.R. Escribano and R.J. Wiesner. 1994. Stoichiometry of mitochondrial transcripts and regulation of gene expression by mitochondrial transcription factor A. Biochem. Biophys. Res. Commun. 200:619-626.
4. Hood, D.A., R. Zak and D. Pette. 1989. Chronic stimulation of rat skeletal muscle induces coordinate increases in mitochondrial and nuclear mRNAs of cytochrome c oxidase. Eur. J. Biochem. 179:275-280.
5. Lubin, M.B., J.D. Elashoff, S.-J. Wang, J.I. Rotter and H. Toyoda. 1991. Precise gene dosage determination by polymerase chain reaction: theory, methodology, and statistical approach. Mol. Cell. Probes 5:307-317.
6. Raeymaekers, L. 1993. Quantitative PCR: theoretical considerations with practical implications. Anal. Biochem. 214:582-585.
7. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
8. Sbisa, E., M. Nardelli, F. Tanzariello and C. Saccone. 1990. The complete and symmetrical transcription of the main non coding region of rat mitochondrial genome: in vivo mapping of heavy and light transcripts. Curr. Genet. 17:247-253.
9. Volkenandt, M., A.P. Dicker, D. Banerjee, R. Fanin, B. Schweitzer, T. Horikoshi, K. Danenberg, P. Danenberg and J.R. Bertino. 1992. Quantitation of gene copy number and mRNA using the polymerase chain reaction. Proc. Soc. Exp. Biol. Med. 200:1-6.
10. Wiesner, R.J., T. Kurowski and R. Zak. 1992. Regulation by thyroid hormone of nuclear and mitochondrial genes encoding subunits of cytochrome c oxidase in rat liver and skeletal muscle. Mol. Endocrinol. 6:1458-1467.
11. Wiesner, R.J., J.C. Rüegg, and I. Morano. 1992. Counting target molecules by exponential polymerase chain reaction: copy number of mitochondrial DNA in rat tissues. Biochem. Biophys. Res. Commun. 183:553-559.
12. Zenilman, M.E., W. Graham, K. Tanner and A.R. Shuldiner. 1995. Competitive reverse transcription - polymerase chain reaction without an artificial internal standard. Anal. Biochem. 224:339-346.