Site-specific incorporation of unnatural amino acids into receptors expressed in mammalian cells

Chemistry and Biology

Volumn 10, Issue 6, 2003, Pages 573-580

  Monahan, Sarah L ^a   Lester, Henry A ^a   Dougherty, Dennis A ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; CRICETINAE; MAMMALIA;

EID: 0038506126     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(03)00124-8     Document Type: Article

References (25)

1. Cornish V.W., Benson D.R., Altenbach C.A., Hideg K., Hubbell W.L., Schultz P.G. Site-specific incorporation of biophysical probes into proteins. Proc. Natl. Acad. Sci. USA. 91:1994;2910-2914.
2. Cornish V.W., Mendel D., Schultz P.G. Probing protein structure and function with an expanded genetic code. Angew. Chem. Int. Ed. Engl. 34:1995;621-633.
3. Dougherty D.A. Unnatural amino acids as probes of protein structure and function. Curr. Opin. Chem. Biol. 4:2000;645-652.
4. Gillmore M.A., Steward L.E., Chamberlin A.R. Incorporation of noncoded amino acids by in vitro protein biosynthesis. Top. Curr. Chem. 202:1999;77-99.
5. Nowak M.W., Gallivan J.P., Silverman S.K., Labarca C.F., Dougherty D.A., Lester H.A. In vivo incorporation of unnatural amino acids into ion channels in a Xenopus oocyte expression system. Methods Enzymol. 293:1998;504-529.
6. Nowak M.W., Kearney P.C., Sampson J.R., Saks M.E., Labarca C.G., Silverman S.K., Zhong W., Thorson J., Abelson J.N., Davidson N.et al. Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science. 268:1995;439-442.
7. Li L.T., Zhong W.G., Zacharias N., Gibbs C., Lester H.A., Dougherty D.A. The tethered agonist approach to mapping ion channel proteins toward a structural model for the agonist binding site of the nicotinic acetylcholine receptor. Chem. Biol. 8:2001;47-58.
8. Petersson E.J., Choi A., Dahan D.S., Lester H.A., Dougherty D.A. A perturbed pK(a) at the binding site of the nicotinic acetylcholine receptor. implications for nicotine binding J. Am. Chem. Soc. 124:2002;12662-12663.
9. Kearney P.C., Zhang H., Zhong W., Dougherty D.A., Lester H.A. Determinants of nicotinic receptor gating in natural and unnatural side chain structures at the M2 9′ position. Neuron. 17:1996;1221-1229.
10. Miller J.C., Silverman S.K., England P.M., Dougherty D.A., Lester H.A. Flash decaging of tyrosine sidechains in an ion channel. Neuron. 20:1998;619-624.
11. Zhong W., Gallivan J.P., Zhang Y., Li L., Lester H.A., Dougherty D.A. From ab initio quantum mechanics to molecular neurobiology. a cation-π binding site in the nicotinic receptor Proc. Natl. Acad. Sci. USA. 95:1998;12088-12093.
12. Beene D., Brandt G., Zhong W., Zacharias N., Lester H., Dougherty D. Cation-π interactions in ligand recognition by serotonergic (5-HT3A) and nicotinic acetylcholine receptors. the anomalous binding properties of nicotine Biochemistry. 41:2002;10262-10269.
13. England P.M., Lester H.A., Davidson N., Dougherty D.A. Site-specific, photochemical proteolysis applied to ion channels in vivo. Proc. Natl. Acad. Sci. USA. 94:1997;11025-11030.
14. Tong Y.H., Brandt G.S., Li M., Shapovalov G., Slimko E., Karschin A., Dougherty D.A., Lester H.A. Tyrosine decaging leads to substantial membrane trafficking during modulation of an inward rectifier potassium channel. J. Gen. Physiol. 117:2001;103-118.
15. Capone J.P., Sharp P.A., RajBhandary U.L. Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene. EMBO J. 4:1985;213-221.
16. Teruel M.N., Meyer T. Electroporation-induced formation of individual calcium entry sites in the cell body and processes of adherent cells. Biophys. J. 73:1997;1785-1796.
17. Teruel M.N., Blanpied T.A., Shen K., Augustine G.J., Meyer T. A versatile microporation technique for the transfection of cultured CNS neurons. J. Neurosci. Methods. 93:1999;37-48.
18. Charnet P., Labarca C., Leonard R.J., Vogelaar N.J., Czyzyk L., Gouin A., Davidson N., Lester H.A. An open-channel blocker interacts with adjacent turns of α-helices in the nicotinic acetylcholine receptor. Neuron. 4:1990;87-95.
19. Labarca C., Nowak M.W., Zhang H.Y., Tang L.X., Deshpande P., Lester H.A. Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Nature. 376:1995;514-516.
20. Saks M.E., Sampson J.R., Nowak M.W., Kearney P.C., Du R., Abelson J.N., Lester H.A., Dougherty D.A. An engineered Tetrahymena tRNAGln for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression. J. Biol. Chem. 271:1996;23169-23175.
21. Negrutskii B.S., Deutscher M.P. Channeling of aminoacyl-tRNA for protein synthesis in vivo. Proc. Natl. Acad. Sci. USA. 88:1991;4991-4995.
22. Kohrer C., Xie L., Kellerer S., Varshney U., RajBhandary U.L. Import of amber and ochre suppressor tRNAs into mammalian cells. a general approach to site-specific insertion of amino acid analogues into proteins Proc. Natl. Acad. Sci. USA. 98:2001;14310-14315.
23. Sakamoto K., Hayashi A., Sakamoto A., Kiga D., Nakayama H., Soma A., Kobayashi T., Kitabatake M., Takio K., Saito K.et al. Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells. Nucleic Acids Res. 30:2002;4692-4699.
24. Ilegems E., Pick H.M., Vogel H. Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery. Nucleic Acids Res. 30:2002;e128.
25. Li Y.X., Zhang Y., Lester H.A., Schuman E.M., Davidson N. Enhancement of neurotransmitter release induced by brain-derived neurotrophic factor in cultured hippocampal neurons. J. Neurosci. 18:1998;10231-10240.