A versatile microporation technique for the transfection of cultured CNS neurons

Journal of Neuroscience Methods

Volumn 93, Issue 1, 1999, Pages 37-48

  Teruel, Mary N ^a   Blanpied, Thomas A ^b   Shen, Kang ^a   Augustine, George J ^b   Meyer, Tobias ^a  

^a DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

CFP; Electroporation; GFP; Hippocampal neurons; Quantitative co transfection; RNA; YFP

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; CELL FUNCTION; CONTROLLED STUDY; DEVICE; DNA TRANSFECTION; ELECTROPORATION; GENETIC TRANSFECTION; HIPPOCAMPUS; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SYNAPTIC TRANSMISSION; TECHNIQUE;

ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; DNA, COMPLEMENTARY; ELECTROPORATION; HIPPOCAMPUS; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RNA; TRANSFECTION;

EID: 0032728993     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00112-0     Document Type: Article

References (27)

1. Bekkers J.M., Stevens C.F. Excitatory and inhibitory autaptic currents in isolated hippocampal neurons in cell culture. Proc. Natl. Acad. Sci. USA. 88:1991;7834-7838.
2. Cormack B.P., Valdivia R.H., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 173:1996;33-38.
3. Haas J., Park E.C., Seed B. Codon usage limitations in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6:1996;315-324.
4. Heim R., Tsien R.Y. Engineering green fluorescent proteins for improved brightness, longer wavelengths and fluoresence energy transfer. Curr. Biol. 6:1996;178-182.
5. Holt C.E., Garlick N., Cornel E. Lipofection of cDNAs in the embryonic vertebrate central nervous system. Neuron. 4:1990;203-214.
6. Horne J., Meyer T. Elementary calcium release units induced by inositol trisphosphate. Science. 276:1997;1690-1693.
7. 2+ release from intracellular stores. J. Neurosci. 17:1997;4129-4135.
8. Kaang B.K., Kandel E.R., Grant S.G. Activation of cAMP-responsive genes by stimuli that produce long-term facilitation in Aplysia sensory neurons. Neuron. 10:1993;427-435.
9. Liley A.W., North K.A.K. An electrical investigation of effects of repetitive stimulation on mammalian neuromuscular junction. J. Neurophysiol. 16:1968;509-527.
10. Lo D.C., McAllister A.K., Katz L.C. Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron. 13:1994;1263-1268.
11. Moriyoshi K., Richards L.J., Akazawa C., O'Leary D.D., Nakanishi S. Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP. Neuron. 16:1996;255-260.
12. Oancea E., Teruel M.N., Quest A.F.G., Meyer T. GFP-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J. Cell Biol. 140:1998;1-14.
13. Pettit D.L., Koothan T., Liao D., Malinow R. Vaccinia virus transfection of hippocampal slice neurons. Neuron. 14:1995;685-688.
14. Resh M.D. Myristylation and palmitylation of Src family members: the fats of the matter. Cell. 76:1994;411-413.
15. Rosenmund C., Stevens C.F. Definition of the readily releasable pool of vesicles at hippocampal synapses. Neuron. 16:1996;1197-1207.
16. Ryan T.A., Smith S.J. Vesicle pool mobilization during action potential firing at hippocampal synapses. Neuron. 14:1995;983-989.
17. 2+/Calmodulin dependent protein kinase IIa: domain requirement and in vivo mobility of oligomer. J. Neurochem 1998;70:96-104.
18. Stauffer T.P., Martenson C.H., Rider J.E., Kay B.K., Meyer T. Inhibition of Lyn function in mast cell activation by SH3 domain binding peptides. Biochemistry. 36:1997;9388-9394.
19. Stevens C.F., Wang Y. Facilitation and depression at single central synapses. Neuron. 14:1995;795-802.
20. Subramanian K., Meyer T. Calcium-induced changes in the structure of endoplasmic reticulum and nuclear envelope calcium stores. Cell. 89:1997;963-971.
21. Swandulla D., Hans M., Zipser K., Augustine G.J. Role of residual calcium in synaptic depression and posttetanic potentiation: fast and slow calcium signalling in nerve terminals. Neuron. 7:1991;915-926.
22. Terasaki M., Slater N.T., Fein A., Schmidek A., Reese T.S. Continuous network of endoplasmic reticulum in cerebellar Purkinje neurons. Proc. Natl. Acad. Sci. USA. 91:1994;7510-7514.
23. Teruel M.N., Meyer T. Electroporation induced formation of calcium entry sites in cell body and processes of adherent cells. Biophys. J. 73:1997;1785-1796.
24. Thies R.E. Neuromuscular depression and the apparent depletion of transmitter in mammalian muscle. J. Neurophysiol. 28:1965;427-442.
25. Tong G., Jahr C.E. Multivesicular release from excitatory synapses of cultured hippocampal neurons. Neuron. 12:1994;51-59.
26. Xia Z.G., Dudek H., Miranti C.K., Greenberg M.E. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J. Neurosci. 16:1996;5425-5436.
27. Yokoe H., Meyer T. Spatial dynamics of GFP-tagged proteins investigated by local flourescence enhancement. Nat. Biotechnol. 14:1996;1252-1256.