Extracellular metabolism of nucleotides in neuroblastoma × glioma NG108-15 cells determined by capillary electrophoresis

Cellular and Molecular Neurobiology

Volumn 23, Issue 3, 2003, Pages 349-364

  Kaulich, Marko ^a   Qurishi, Ramatullah ^a   Müller, Christa E ^a,b  

^a UNIVERSITY OF BONN   (Germany)

^b Pharmazeutisches Institut   (Germany)

Author keywords

Adenylate kinase; Alkaline phosphatase; Capillary electrophoresis; Dipyridamole; Ectoenzymes; Levamisole; NG108 15 cells; Nucleotide metabolism

Indexed keywords

ADENOSINE; ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATASE INHIBITOR; ADENOSINE TRIPHOSPHATE; ADENYLATE KINASE; ALKALINE PHOSPHATASE; BETA,GAMMA DIBROMOMETHYLENE 6 N,N DIETHYLADENOSINE TRIPHOSPHATE; DIPYRIDAMOLE; LEVAMISOLE; NUCLEOTIDE; URACIL; URIDINE; URIDINE DIPHOSPHATE; URIDINE PHOSPHATE; URIDINE TRIPHOSPHATE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CAPILLARY ZONE ELECTROPHORESIS; CERVICAL GANGLION; CONTROLLED STUDY; GLIOMA; HUMAN; HUMAN CELL; HYBRID CELL; HYDROLYSIS; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; NEUROBLASTOMA; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

ADENOSINE; ALKALINE PHOSPHATASE; ANIMALS; CHROMATOGRAPHY, MICELLAR ELECTROKINETIC CAPILLARY; DIPYRIDAMOLE; ELECTROPHORESIS, CAPILLARY; EXTRACELLULAR SPACE; GLIOMA; MICE; NERVOUS SYSTEM; NEUROBLASTOMA; NEURONS; NUCLEOTIDES; PHOSPHODIESTERASE INHIBITORS; PHOSPHORYLATION; PURINES; PYRIMIDINES; RATS; REACTION TIME; TUMOR CELLS, CULTURED; URACIL;

EID: 0038390356     PISSN: 02724340     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1023640721630     Document Type: Article

References (32)

1. Bhagwat, S. S., and Williams, M. (1997). P2 Purine and pyrimidine receptors: Emerging superfamilies of G-protein-coupled and ligand-gated ion channel receptors. Eur. J. Chem. 32:183-193.
2. + conductance in Xenopus oocytes by expression of adenylate kinase cloned from renal proximal tubules. Biochim. Biophys. Acta 1510:29-42.
3. Connolly, G. P., and Duley, J. A. (2000). Ecto-nucleotidase of cultured rat superior cervical ganglia: Dipyridamole is a novel inhibitor. Eur. J. Pharmacol. 397:271-277.
4. Grobben, B., Anciaux, K., Roymans, D., Stefan, C., Bollen, M., Esmans, E. L., and Slegers, H. (1999). An ecto-nucleotide pyrophosphatase is one of the main enzymes involved in the extracellular metabolism of ATP in rat C6 glioma. J. Neurochem. 72:826-834.
5. 3H]nitrobenzylthioinosine binding site. Mol. Pharmacol. 39:771-779.
6. Harden, T. K., Lazarowski, E. R., and Boucher, R. C. (1997). Release, metabolism and interconversion of adenine and uridine nucleotides: Implications for G protein-coupled P2 receptor agonist selectivity. Trends Pharmacol. Sci. 18:43-46.
7. Heilbronn, E., Knoblauch, B. H. A., and Müller, C. E. (1997). Uridine nucleotide receptors and their ligands: Structural, physiological, and pathophysiological aspects, with special emphasis on the nervous system. Neurochem. Res. 22:1041-1050.
8. Jarvis, S. M., Janmohamed, S. N., and Young, J. D. (1983). Kinetics of nitrobenzylthioinosine binding to the human erythrocyte nucleoside transporter. Biochem. J. 216:661-667.
9. Kimura, T., Ho, I. K., and Yamamoto, I. (2001). Uridine receptor: Discoverey and its involvement in sleep mechanism. Sleep 24:251-260.
10. King, B. A., Townsend-Nicholson, A., and Burnstock, G. (1998). Metabotropic receptors for ATP and UTP: Exploring the correspondence between native and recombinant nucleotide receptors. Trends Pharmacol. Sci. 19:506-514.
11. 2 receptor agonists. Eur. J. Med. Chem. 34:809-824.
12. Lazarowski, E. R., Boucher, R. C., and Harden, T. K. (2000). Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations. J. Biol. Chem. 275:31061-31068.
13. Lazarowski, E. R., and Harden, T. K. (1999). Quantitation of extracellular UTP using a sensitive enzymatic assay. Br. J. Pharmacol. 127:1272-1278.
14. Lazarowski, E. R., Homolya, L., Boucher, R. C., and Harden, T. K. (1997). Identification of an ectonucleoside diphosphokinase and its contribution to interconversion of P2 receptor agonists. J. Biol. Chem. 272:20402-20407.
15. 2U receptor in neuroblastoma x glioma (NG108-15) cells. J. Neurochem. 60:1115-1125.
16. Matsuoka, I., Zhuo, Y., Ishimoto, H., and Nakanishi, H. (1995). Extracellular ATP stimulates adenylyl cyclase and phospholipase C through distinct purinoceptors in NG108-15 cells. Mol. Pharmacol. 47:855-862.
17. Meghji, P., Pearson, J. D., and Slakey, L. L. (1995). Kinetics of extracellular ATP hydrolysis by microvascular endothelial cells from rat heart. Biochem. J. 308:725-731.
18. 2b adenosine receptors in NG108-15 cells. Biochem. Pharmacol. 55:595-603.
19. Nagy, A. K., Shuster, T. A., and Delgado-Escueta, A. V. (1989). Rat brain synaptosomal ATP:AMP-phosphotransferase activity. J. Neurochem. 53:1166-1172.
20. 2 Receptors: Identification of a UDP-selective, a UTP-selective, and an ATP- and UTP-specific receptor. Mol. Pharmacol. 50:224-229.
21. Ohkubo, S., Kimura, J., and Matsuoka, I. (2000a). Ecto-alkaline phosphatase in NG108-15 cells: A key enzyme mediating P1 antagonist-sensitive ATP response. Br. J. Pharmacol. 131:1667-1672.
22. Ohkubo, S., Kumura, J., and Matsuoka, I. (2000b). Correlation between adenine nucleotide-induced cyclic AMP elevation and extracellular adenosine formation in NG108-15 cells. Jpn. J. Pharmacol. 84:325-333.
23. Qurishi, R., Kaulich, M., and Müller, C. E. (2002). Fast, efficient capillary electrophoresis method for measuring nucleotide degradation and metabolism. J. Chromatogr. A 952:275-281.
24. Ralevic, V., and Burnstock, G. (1998). Receptors for purines and pyrimidines. Pharmacol. Rev. 50:413-492.
25. 2+- and nitric oxide-dependent stimulation of cyclic GMP synthesis in neuronal cell line induced by P2 purinergic/pyrimidinergic receptor. J. Neurochem. 64:61-68.
26. Sak, K., Samuel, K., Kelve, M., and Webb, T. E. (2001). Pharmacological characterization of pyrimidinoceptor responses in NG108-15 cells. Eur. J. Pharmacol. 415:127-133.
27. Todorov, L. D., Mihaylova-Todorova, S., Westfall, T. D., Sneddon, P., Kennedy, C., Bjur, R. A., and Westfall, D. P. (1997). Neuronal release of soluble nucleotidases and their role in neurotransmitter inactivation. Nature 387:76-79.
28. Torres, M., Pintor, J., and Miras-Portugal, M. T. (1990). Presence of ectonucleotidases in cultured chromaffin cells: Hydrolysis of extracellular adenine nucleotides. Arch. Biochem. Biophys. 279:37-44.
29. Yao, S. Y. M., Ng, A. M. L., Muzyka, W. R., Griffiths, W. R., Cass, C. E., Baldwin, S. A., and Young, J. D. (1997). Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transproter proteins (rENT1 and rENT2) from rat tissues. J. Biol. Chem. 272:28430-28432.
30. Yegutkin, G., Bodin, P., and Burnstock, G. (2000). Effect of shear stress on the release of soluble ectoenzymes ATPase and 5′-nucleotidase along with endogenous ATP from vascular endothelial cells. Br. J. Pharmacol. 29:921-926.
31. Yegutkin, G., Henttinen, T., and Jlakanen, S. (2001). Extracellular ATP formation on vascular endothelial cells is mediated by ecto-nucleotide kinase activities via phosphotransfer reactions. FASEB J. 15:251-260.
32. Zimmermann, H. (2000). Extracellular metabolism of ATP and other nucleotides. Naunyn Schmiedebergs Arch. Pharmacol. 362:299-309.