Quantitation of extracellular UTP using a sensitive enzymatic assay

British Journal of Pharmacology

Volumn 127, Issue 5, 1999, Pages 1272-1278

  Lazarowski, Eduardo R ^a   Harden, T Kendall ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Extracellular nucleotides; P2Y receptors; UTP measurement; UTP release

Indexed keywords

ADENOSINE TRIPHOSPHATE; GLUCOSE 1 PHOSPHATE; PURINE P2Y RECEPTOR; THROMBIN; URIDINE DIPHOSPHATE GLUCOSE; URIDINE TRIPHOSPHATE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL LINE; CONTROLLED STUDY; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; LEUKOCYTE; NONHUMAN; PRIORITY JOURNAL; RAT; RESPIRATORY EPITHELIUM; THROMBOCYTE;

ANIMALS; RATS; RECEPTORS, PURINERGIC P2; TUMOR CELLS, CULTURED; URIDINE DIPHOSPHATE GLUCOSE; URIDINE TRIPHOSPHATE; UTP-GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE;

EID: 0032803745     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0702654     Document Type: Article

References (35)

1. ABBRACCHIO, M.P., CERUTI, S., BOLEGO, C., PUGLISI, L., BURNSTOCK, G. & CATTABENI, F. (1996). Trophic roles of P2 purinoceptors in central nervous system astroglial cells. Ciba Found. Symp., 198, 142-147.
2. ANDERSON, C.M. & PARKINSON, F.E. (1997). Potential signalling roles for UTP and UDP: sources, regulation and release of uracil nucleotides. Trends Pharmacol. Sci., 18, 387-392.
3. BEIGI, R., KOBATAKE, M., AIZAWA, M. & DUBYAK, G.R. (1999). Detection of local ATP release from activated platelets using cell surface-attached firefly luciferase. Am. J. Physiol., 276, C267-C278.
4. BOGDANOV, Y.D., DALE, L., KING, B.F., WHITTOCK, N. & BURNSTOCK, G. (1997). Early expression of a novel nucleotide receptor in the neural plate of Xenopus embryos. J. Biol. Chem., 272, 12583-12590.
5. BOGDANOV, Y.D., WILDMAN, S.S., CLEMENTS, M.P., KING, B.F. & BURNSTOCK, G. (1998). Molecular cloning and characterization of rat P2Y4 nucleotide receptor. Br. J. Pharmacol., 124, 428-430.
6. BOYER, J.L., WALDO, G.L. & HARDEN, T.K. (1997). Molecular cloning and expression of an avian G protein-coupled P2Y receptor. Mol. Pharmacol., 52, 928-934.
7. BROWN, H.A., LAZAROWSKI, E.R., BOUCHER, R.C. & HARDEN, T.K. (1991). Evidence that UTP and ATP regulate phospholipase C through a common extracellular 5′-nucleotide receptor in human airway epithelial cells. Mol. Pharmacol., 40, 648-655.
8. BURNSTOCK, G. (1972). Purinergic nerves. Pharmacol. Rev., 24, 509-581.
9. 2-purinoceptors? Gen. Pharmacol., 16, 433-440.
10. 2 nucleotide receptor. J. Biol. Chem., 270, 26152-26158.
11. CLIFFORD, E.E., MARTIN, K.A., DALAL, P., THOMAS, R. & DUBYAK, G.R. (1997). Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5′-nucleotidase in myeloid leukocytes. Am. J. Physiol., 273, C973-C987.
12. COHEN, B., LIU, Y., DRUKER, B., ROBERTS, T.M. & SCHAFFHAUSEN, B.S. (1990). Characterization of pp85, a target of oncogenes and growth factor receptors. Mol. Cell. Biol., 10, 2909-2915.
13. COMMUNI, D., PIROTTON, S., PARMENTIER, M. & BOEYNAEMS, J.M. (1995). Cloning and functional expression of a human uridine nucleotide receptor. J. Biol. Chem., 270, 30849-30852.
14. 2-purinergic receptors for extracellular ATP and other nucleotides. Am. J. Physiol., 265, C577-C606.
15. DUGGLEBY, R.G., CHAO, Y.C., HUANG, J.G., PENG, H.-L. & CHANG, H.-Y. (1996). Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic properties of the recombinant enzymes expressed in Escherichia coli. Eur. J. Biochem., 235, 173-179.
16. FREDHOLM, B.B., ABBRACCHIO, M.P., BURNSTOCK, G., DUBYAK, G.R., HARDEN, T.K., JACOBSON, K.A., SCHWABE, U & WILLIAMS, M. (1997). Towards a revised nomenclature for P1 and P2 receptors. Trends Pharmacol. Sci., 18, 79-82.
17. 1 guanine-and uridine-5′-phosphonucleotides in blood platelets and storage organelles of various species. J. Pharmacol. Exp. Ther., 178, 210-215.
18. GORDON, J.L. (1986). Extracellular ATP: effects, sources and fate. Biochem. J., 233, 309-319.
19. HANSEN, R.G., ALBRECHT, G.J., BASS, S.T. & SEIFERT, L.L. (1966). UDP-Glucose pyrophosphorylase (crystalline) from liver. In Complex Carbohydrates, eds. Neufeld, E.F. & Ginsburg, V. pp. 248-253. Academic Press. New York, NY, U.S.A.
20. KEPPLER, D., RUDIGER, J. & DECKER, K. (1970). Enzymic determination of uracil nucleotides in tissues. Anal. Biochem., 38, 105-114.
21. KNOWLES, M.R., CLARKE, L.L. & BOUCHER, R.C. (1991). Extracellular nucleotides activate chloride secretion in cystic fibrosis airway epithelia in vivo. N. Engl. J. Med., 325, 533-538.
22. LAZAROWSKI, E.R. & HARDEN, T.K. (1994). Identification of a uridine nucleotide-selective G-protein-linked receptor that activates phospholipase C. J. Biol. Chem., 269, 11830-11836.
23. LAZAROWSKI, E.R. & HOMOLYA, L., BOUCHER, R.C. & HARDEN, T.K. (1997a). Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J. Biol. Chem., 272, 24348-24354.
24. LAZAROWSKI, E.R., HOMOLYA, L., BOUCHER, R.C. & HARDEN, T.K. (1997b). Identification of an ecto-nucleoside diphosphokinase and its contribution to interconversion of P2 receptor agonists. J. Biol. Chem., 272, 20402-20407.
25. 2 receptor. Proc. Natl. Acad. Sci. U.S.A., 94, 2599-2603.
26. LELOIR, L.F. (1971). Two decades of research on the biosynthesis of saccharides. Science, 172, 1299-1303.
27. LUSTIG, K.D., SHIAU, A.K., BRAKE, A.J. & JULIUS, D. (1993). Expression cloning of an ATP receptor from mouse neuroblastoma cells. Proc. Natl. Acad. Sci. U.S.A., 90, 5113-5117.
28. MASON, S.J., PARADISO, A.M. & BOUCHER, R.C. (1991). Regulation of transepithelial ion transport and intracellular calcium by extracellular adenosine triphosphate in normal and cystic fibrosis airway epithelium. Br. J. Pharmacol., 103, 1649-1656.
29. NGUYEN, T., ERB, L., WEISMAN, G.A., MARCHESE, A., HENG, H.H.Q., GARRAD, R.C., GEORGE, S.R., TURNER, J.T. & O'DOWD, B.F. (1995). Cloning, expression, and chromosomal localization of the human uridine nucleotide receptor gene. J. Biol. Chem., 270, 30845-30848.
30. 2 receptors: Identification of a UDP-selective, a UTP-selective, and an ATP- and UTP-specific receptor. Mol. Pharmacol., 50, 224-229.
31. 2U nucleotide receptor, a target for cystic fibrosis pharmacology. Proc. Natl. Acad. Sci. U.S.A., 91, 3275-3279.
32. SAIAG, B., BODIN, P., SHACOORI, V., CATHELINE, M., RAULT, B. & BURNSTOCK, G. (1995). Uptake and flow-induced release of uridine nucleotides from isolated vascular endothelial cells. Endothelium, 2, 279-285.
33. SASVARI-SZEKELY, M., VITEZ, M., STAUB, M. & ANTONI, F. (1975). Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase. Biochem. Biophys. Acta., 395, 221-228.
34. VERGHESE, M.W., KNEISLER, T.B. & BOUCHERON, J.A. (1996). P2U agonists induce chemotaxis and actin polymerization in human neutrophils and differentiated HL60 cells. J. Biol. Chem., 271, 15597-15601.
35. WATT, W.C., LAZAROWSKI, E.R. & BOUCHER, R.C. (1998). Cystic fibrosis transmembrane regulator-independent release of ATP. J. Biol. Chem., 273, 14053-14058.